Re: [Histonet] PFA Fixed tissue not sticking to slides?

[Due, Brice via Histonet]

Hello Jennifer, yes over-fixation will cause adhesion problems. This applies to 
formalin fixed frozens as well as PFA fixed frozens.

I have had good luck using chromated gelatin to coat slides. I have 
successfully cut and stained lung specimens left in NBF for several months. 
Commercially, chromated gelatin is sold as STA-ON. I believe Leica now owns it, 
but you can make your own quite easily.

I tend to use STA-ON full strength, or minimally diluted. Spread some on a 
slide with a transfer pipette, taking care not to allow it to run onto the back 
of the slide. Shake off the excess and set vertical to dry. The coating MUST be 
dry before you use the slide to pick up sections. Freshly made slides work best.

After pick up, it is important to air-dry the section completely before 
immersing it in any solution. The moisture content of the frozen section must 
evaporate or the chromated gelatin will not hold the tissue. Don't worry, fixed 
sections do not suffer from artifacts when air-dried because the morphology is 
already locked by the fixation.

Best luck!
-brice






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Re: [Histonet] Microm HM 505 E Cryostat

[Johanna Haddock via Histonet]

Thank you very much.  I received a copy of the document.

Johanna Haddock, BS, MT(ASCP)
QA Coordinator
ProPath - The Leader in Pathology Services
1355 River Bend Dr.
Dallas, TX 75247
214-237-1623
214-237-1731 Fax

To learn more about ProPath, please visit 
http://www.ProPath.com

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From: Johanna Haddock
Sent: Tuesday, August 09, 2016 2:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: FW: Microm HM 505 E Cryostat

Hi,

Does anyone has the Manufacturer Instruction Manual for the Microm HM 505 E 
Cryostat  that  you can share with me?

Thanks,


Johanna Haddock, BS, MT(ASCP)
QA Coordinator
ProPath - The Leader in Pathology Services
1355 River Bend Dr.
Dallas, TX 75247

To learn more about ProPath, please visit 
http://www.ProPath.com

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you are not the intended recipient, you are hereby notified  that any 
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strictly prohibited. If you have received this message in  error or are not the 
named recipient, please notify us immediately by  contacting the sender at the 
electronic mail address noted above, and  delete and destroy all copies of this 
message



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[Histonet] FW: Microm HM 505 E Cryostat

[Johanna Haddock via Histonet]

Hi,

Does anyone has the Manufacturer Instruction Manual for the Microm HM 505 E 
Cryostat  that  you can share with me?

Thanks,


Johanna Haddock, BS, MT(ASCP)
QA Coordinator
ProPath - The Leader in Pathology Services
1355 River Bend Dr.
Dallas, TX 75247

To learn more about ProPath, please visit 
http://www.ProPath.com

" This electronic message is intended to be for the use only of the  named 
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you are not the intended recipient, you are hereby notified  that any 
disclosure, copying, distribution, or use of the contents of this  message is 
strictly prohibited. If you have received this message in  error or are not the 
named recipient, please notify us immediately by  contacting the sender at the 
electronic mail address noted above, and  delete and destroy all copies of this 
message



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[Histonet] Cryostat sectioning of frozen leaves

[Jo-Ann Bader, Ms. via Histonet]

I am trying to cut frozen leaf sections at 6 and 8 microns.  All I am getting 
is bunched up leaf.  What is the trick/  I am cutting at -20 should be cutting 
at a lower temp and cutting thicker?


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270
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Re: [Histonet] productivity standards/slide

[Morken, Timothy via Histonet]

That's a good question, but tough to answer because every lab has different 
ways of mounting sections. I think you will have to define that for your own 
workflow. I've seen labs that require 30 serial sections on a slide for certain 
specimens, so what is that?!

You could start with the one section/one slide as the base line, then determine 
how much extra time it takes to mount levels (cutting between sections taken), 
or other types of mounting. Then you can apply a multiplier to those types of 
slides and come up with a reasonable productivity guideline.


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center




-Original Message-
From: Kienitz, Kari via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, August 09, 2016 10:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] productivity standards/slide

Hi everyone,

What constitutes a slide? For volume purposes/tech productivity.

1 section on a slide= 1 slide
3 sections (levels) on a slide=1 slide or does it equal 3 slides?




Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
 NE 99th Ave
Portland, OR  97220
503.935.8311
kkien...@orclinic.com




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[Histonet] productivity standards/slide

[Kienitz, Kari via Histonet]

Hi everyone,

What constitutes a slide? For volume purposes/tech productivity.

1 section on a slide= 1 slide
3 sections (levels) on a slide=1 slide or does it equal 3 slides?




Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
 NE 99th Ave
Portland, OR  97220
503.935.8311
kkien...@orclinic.com




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[Histonet] QAQC Procedure:

[J B via Histonet]

Is anyone willing to share a procedure for QAQC? I have a procedure that is
extremely detailed and our physicans would like a shorter version.

I would love to see what others have in practice if you are willing to
share it is greatly appreciated.

Sincerely,

JB
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Re: [Histonet] Pathology Ladder System

[Morken, Timothy via Histonet]

Our career ladder at UCSF

HLT 1 thru 4 (Hospital Laboratory Technician) for non-histology positions. 
HLT-1 is entry level, could be out of high school. To move up requires more 
education. HLT-2 AA to BA/BA, HLT-3, -4, BA/BS used as accessioners, basic lab 
techs in grossing and cytology, running stainers, changing tissue processors in 
histology, etc. HLT-1,-2 are bench techs. HLT-3 is a Senior tech, HLT-4 is a 
Lead tech. HLT-3 and -4 can be trained for grossing. They can actually work 
their way thru to minor grossing with the right qualifications. If trained for 
histotechnology or more advanced grossing ie, PA-level) we promote them to the 
Histotechnologist series.

HT 1 thru 4 (Histotechnologist). Traditional histology lab techs as well as 
on-the-job trained Pathologist's Assistants (HT-3,-4 only). HT-1,2 are the 
basic bench techs, HT-3, is Senior tech, HT-4 is Lead tech. Only HT-4 and 
Supervisor requires ASCP certification for histology work (not grossing work), 
but  is "preferred" at the other levels. So far all our histotechs are ASCP 
certified HT or HTL.  None of the OJT PA's are certified (though they could 
work for it if they desired). We do have an advantage of a large research 
population to draw techs from so all come to us with good educational 
background and have learned some histology in a research lab. 

These are all union positions and each classification (i.e HT-1 or HT-2) has 12 
annual steps (controlled by the union, not administration) before maxing out in 
pay. At that point they reach a ceiling and can only move up by going to the 
next up classification. We usually require 5 years in one step  before 
reclassifying unless the person is exceptional. As a manager I can only 
reclassify; I cannot move someone up a step as a reward for good work.

This works out pretty well for advancement but we have a fairly large 
organization - 12 histotechs and 2 HLT in the histo lab; 4 HLT in cyto lab; 4 
certifed PA's; 4 OJT PA's plus 5 HLT accessioners - moving up or to other 
positions within the department is possible.  Several of our accessioners have 
moved up to Histotech positions, and one OJT PA went to PA school and came back 
to work for us. Another HLT accessioner is applying to PA school.

We also now hire only certified PA's, and try  to hire program-trained PA's. 
the competition for that is tough and for recent positions we have essentially 
opened it to any certified PA.

Cyto uses the HLT series for lab work and has another Cytotechnologist series 
for cytotechs, same idea of level 1-4. 

At the top of each lab is the supervisor in each discipline, one for cytology, 
histology, grossing and administration.

The usual problem in Histo labs is that people stay a long time and so block 
others from moving up, so even if a career ladder is in place advancement is 
difficult. Unless the department expands the opportunities to advance are 
limited in many labs. 

I histology we have had quite a lot of turnover the last 5 years because we had 
a 4 histotechs retire within a couple years so some advanced and some new techs 
came in. We also opened a couple new jobs. So we now have a fairly young lab 
with a lot of very good techs.


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: Steve via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, August 09, 2016 4:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pathology Ladder System

Good morning everyone.  I had a meeting recently with hospital administration 
where I was asked what leadership could do to retain and keep quality staff.  
Besides the obvious, pay increase's, I suggested a ladder type progression 
system for all lab staff, similar to what some hospitals offer nursing staff.  
My question to this group is, if any of you are using such a system would you 
mind sharing it with me?

Thanks,

Stephen Clark  HT, BS, DESSO
Pathology Dept. Supervisor
Laboratory Safety Officer
Grand Strand Medical Center
809 82nd Parkway
Myrtle Beach, SC 29579
Desk: 843-692-1459
Lab: 843-692-1486
Fax: 843-449-6213

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Re: [Histonet] Pathology Ladder System

[Anne Murvosh via Histonet]

If you don't already have it you may want to offer a shift differential for 
very early or late shifts and weekends.
This makes it a little easier to hire for and keep people on the harder shifts. 
 Anne

-Original Message-
From: Steve via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, August 09, 2016 4:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pathology Ladder System

Good morning everyone.  I had a meeting recently with hospital administration 
where I was asked what leadership could do to retain and keep quality staff.  
Besides the obvious, pay increase's, I suggested a ladder type progression 
system for all lab staff, similar to what some hospitals offer nursing staff.  
My question to this group is, if any of you are using such a system would you 
mind sharing it with me?

Thanks,

Stephen Clark  HT, BS, DESSO
Pathology Dept. Supervisor
Laboratory Safety Officer
Grand Strand Medical Center
809 82nd Parkway
Myrtle Beach, SC 29579
Desk: 843-692-1459
Lab: 843-692-1486
Fax: 843-449-6213

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[Histonet] JOH journal collection

[Connolly, Brett M via Histonet]

HI all,

Is anyone interested in old issues of JOH? My collection spans  1997 - 2016. I 
am missing a few (~ 7).

Let me know and I'll send you a list of what I have.

Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

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for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
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[Histonet] **Histology Job Opportunities**

[Taylor Rinaldi via Histonet]

Hi there!

 

My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. My
team and I specialize specifically in laboratory recruiting nationwide,
working directly with lab managers from different hospitals and reference
laboratories across the US . We are currently recruiting for multiple
Histotechnologist and Histotechnician opportunities. All the opportunities
are fulltime, and permanent. ASCP certification is preferred but not
required for all. 

 

If you or any of your colleagues have been considering a new position in the
Histology field, please don't hesitate to reach out to me directly for
immediate referral and submittal to some of the top hospitals and labs
nationwide.

 

Current states:

Chattanooga, Tennessee 

Sacramento, California

New York, New York  

Atlanta, Georgia

Dallas, Texas

 

Thank you all in advance,

 

Taylor Rinaldi

Recruiting Manager

Prometheus Healthcare 

Office 866-857-1434

  tay...@prometheushealthcare.com

 

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Re: [Histonet] Pathology Ladder System

[Duddey, Aimee via Histonet]

I have had the same discussion with lab administration recently.  I would be 
interested in the same information.

Aimee

-Original Message-
From: Steve via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, August 09, 2016 7:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pathology Ladder System

Good morning everyone.  I had a meeting recently with hospital administration 
where I was asked what leadership could do to retain and keep quality staff.  
Besides the obvious, pay increase's, I suggested a ladder type progression 
system for all lab staff, similar to what some hospitals offer nursing staff.  
My question to this group is, if any of you are using such a system would you 
mind sharing it with me?

Thanks,

Stephen Clark  HT, BS, DESSO
Pathology Dept. Supervisor
Laboratory Safety Officer
Grand Strand Medical Center
809 82nd Parkway
Myrtle Beach, SC 29579
Desk: 843-692-1459
Lab: 843-692-1486
Fax: 843-449-6213

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[Histonet] Pathology Ladder System

[Steve via Histonet]

Good morning everyone.  I had a meeting recently with hospital administration 
where I was asked what leadership could do to retain and keep quality staff.  
Besides the obvious, pay increase's, I suggested a ladder type progression 
system for all lab staff, similar to what some hospitals offer nursing staff.  
My question to this group is, if any of you are using such a system would you 
mind sharing it with me?

Thanks,

Stephen Clark  HT, BS, DESSO
Pathology Dept. Supervisor
Laboratory Safety Officer
Grand Strand Medical Center
809 82nd Parkway
Myrtle Beach, SC 29579
Desk: 843-692-1459
Lab: 843-692-1486
Fax: 843-449-6213

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[Histonet] Decon Processor

[Fawn Bomar via Histonet]

Hi everyone,



Our lab is getting ready to install a new processor.  My question is, how do we 
go about decontaminating our old processor to dispose of it.  The old processor 
is a Ventana Rennaissance.



Thank you

Fawn Bomar
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