[Histonet] Beta-Catenin

[Algeo, Lacie A via Histonet]

Hi All,
What are people using for Beta-catenin control tissue?  Is anyone using tissue 
that is known specifically to have a mutation for the beta-catenin gene?  If 
so, is this something that is purchased?
Thanks,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.al...@providence.org


This message is intended for the sole use of the addressee, and may contain 
information that is priviledged, confidential and exempt from disclosure under 
applicable law.  If you are not the addressee, you are hereby notified that you 
may not use, copy, disclose or distribute to anyone the message or any 
information contained in the message.  If you have received this message in 
error, please immediately advise the sender by reply e-mail and delete this 
message.




This message is intended for the sole use of the addressee, and may contain 
information that is privileged, confidential and exempt from disclosure under 
applicable law. If you are not the addressee you are hereby notified that you 
may not use, copy, disclose, or distribute to anyone the message or any 
information contained in the message. If you have received this message in 
error, please immediately advise the sender by reply email and delete this 
message.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Clip-On Magnifiers for Embedding

[Lisa Hamilton via Histonet]

In the past I have seen clip-on magnifiers that are used to embed tissue. Of
course now that I need  one I cannot remember what catalog I saw them in! I
was hoping someone might

know where I can find them.  

 

Thank You

-Lisa 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Substituting formalin by 70%ethanol in fixed samples.

[Caroline Miller via Histonet]

Hi Julio,

I can speak to a research / core setting where I often advised my users to
place their tissues in 70% ethanol following adequate formalin fixation to
maintain epitopes for potential downstream IHC studies. I have found tissue
can be placed in 70% and maintained indefinitely. They do not 'unfix'.

Recently though I was speaking to a pathologist who doesn't like that
protocol as she sees nuclear changes in tissues that have been in 70% for
extended periods.

So, yes, I think that idea is good in principle. I would say that the staff
time taken, and extra reagent costs, of changing all those biopsies over
into another solution, plus the additional chemical waste generated is not
worth simply making sure the original formalin containers are well sealed
after grossing  and / or purchasing one of those specimen collection
cupboards that filters the air coming through.

I do understand the dangers of formalin, but considering how much of it is
in use and how many of us on this list have been exposed to it for the
majority of our lives and we are still OK! (well, maybe, I suppose that
depends on your definition of OK is :)

Leica seem to think this is OK too!
http://www.leicabiosystems.com/pathologyleaders/fixation-and-fixatives-5-practical-procedures-to-optimise-quality-the-effects-of-heat-and-microwaves/

Ultimately you would have to perform studies to check the effects on your
downstream processes and proof this procedure in your own lab.

yours,
mills



On Sun, Oct 16, 2016 at 10:57 AM, Julio Benavides via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

>
> Hi there,
>
> This is me again with formalin issues. The health and safety officer of my
> institute keeps on in her crusade of eliminating formalin from the world.
> After your helpful emails, I convinced her that you do need formalin to fix
> samples, other approach would need to much setup IHC protocols.  Then and
> with good intention at heart, she has suggested that, one possible solution
> to reduce formalin presence in the institute could be, once samples have
> been fixed in buffered formalin (10%) and embedded in wax, to substitute it
> by 70% ethanol until the sample is discharged. Has anybody tried anything
> similar? What do you think? Would it be possible to come back to formalin
> in case of necessity (let´s say we want to retrim fixed samples after they
> have been in ethanol 70% for 5 months… would you trust them for IHC? HE?).
>
> Thanks again for your help
>
> Julio
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Help with CryoJane Bone cutting

[Owens, Katrina via Histonet]

Hello All:

I recently acquired a CryoJane unit but it doesn't seem to work in regards to 
bone cutting. It works just fine for soft tissue, but getting the bone to 
transfer from the tape to the slide just isn't happening. I have tried, 
successfully, using other UV sources to transfer the bone from the tape to the 
slides, but just not with the CryoJane.

Any help or advice in this matter would be greatly appreciated.

Thank you and have a good day!

Katrina Owens
owen...@ccf.org

===


 Please consider the environment before printing this e-mail

Cleveland Clinic is ranked as one of the top hospitals in America by U.S.News & 
World Report (2015).  
Visit us online at http://www.clevelandclinic.org for a complete listing of our 
services, staff and locations.


Confidentiality Note:  This message is intended for use only by the individual 
or entity to which it is addressed and may contain information that is 
privileged, confidential, and exempt from disclosure under applicable law.  If 
the reader of this message is not the intended recipient or the employee or 
agent responsible for delivering the message to the intended recipient, you are 
hereby notified that any dissemination, distribution or copying of this 
communication is strictly prohibited.  If you have received this communication 
in error,  please contact the sender immediately and destroy the material in 
its entirety, whether electronic or hard copy.  

Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet