Re: [Histonet] Uneven ER/PR

[Terri Braud via Histonet]

My first 2 items to check whenever I have uneven IHC staining are (1) 
Inadequate deparaffinization, (2) bad lot of charged slides - yes this can 
cause terribly streaky or spotty staining, and since you are using the Bond, 
perhaps there was an issue with coverplate placements.
Just things to consider.  Hope this helps, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874

Today's Topics:
   2. ER/PR Uneven Staining (Joanne Clark)
Message: 2
Date: Tue, 29 Nov 2016 21:09:17 +
From: Joanne Clark 
Subject: [Histonet] ER/PR Uneven Staining
I had a breast needle core today that when stained with ER and PR the staining 
was uneven throughout the core, even though the cancer cells were present in 
the entire core.  The specimen had 10 hours fixation in 10% NBF.  I could 
understand the uneven staining from inadequate fixation on large grossed in 
breast tissue, but 10 hours with  needle core biopsies has always been more 
than sufficient.  Does anyone have any ideas?  We use Leica's ER and PR 
antibodies on the BOND.
Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Happy Holidays and Pathology Related Post

[Lester Raff MD via Histonet]

Hope everyone has recovered from Thanksgiving and is looking forward to the 
holidays ahead, whatever you may celebrate.

The new blog post is seeking data on pathology reports. Feel free to read and 
share.

http://www.chicagonow.com/downsize-maybe/2016/11/if-you-have-had-surgery-or-a-biopsy-you-have-had-a-pathology-report-did-you-read-and-understand-it/


Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet