Re: [Histonet] Competency for Supervisors

[Tony Henwood (SCHN) via Histonet]

Hi Mighnon,

I find the so-called competency for all staff, including laboratory managers 
one of the stupidest items ever to be put in a standard (ie ISO 15189).

All staff who perform a test need to be recorded as being competent in it, no 
problem with this but managers as well?

How ridiculous! Why you ask? Well please forgive me in advance for the 
following rant:

The Manager/Supervisor is responsible for instituting the test, training 
his/her staff in the test and importantly responsible for the quality of the 
test and yet His/her competency is called into question?

So how does a Manager obtain a competency record for the test?

1.  Ask a manager from another laboratory (?rival) to sign their 
competency. Could you imagine Donald Trump asking Ed McMahon to sign his 
competency? Since many of us sign confidentiality and privacy agreements, this 
will cause problems.

2.  The manager could ask his staff to sign off his competency. A brilliant 
example of a BAD personal management decision. Why put your staff into the 
stressful position of deciding whether their supervisor is competent or not? If 
they say their supervisor is competent even though they believe they are not, 
then they are forced to lie possibly in order to keep their job. OR in the 
worst case scenario, they could hold this over the supervisor's head (and 
visa-versa) in order to keep their job, obtain favours or monetary gain.

I often wonder whether the authors of this item in the standard were complete 
idiots. They probably never had to face the stresses of managing a laboratory 
and especially valuable scientific staff. 

Absolutely stupid!!

We stepped up our competency assessment by evolving it into a Continuing 
Education exercise, including questions and scenarios for my staff to consider 
and hopefully learn from.

I was then asked why I had not done the same tests. Well I wrote the tests so 
one would assume that I would know the answers - a little self-serving?

Unfortunately I could not test myself when I was attempting my driving licence, 
I would have passed the first time!!

Enough of a rant - please advise whether I am driving up the wrong tree!

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: Mighnon Lashus via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, 2 December 2016 7:38 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Competency for Supervisors

Did anyone have a form for the Histology Supervisor Competency?

Thanks,

Mighnon Lashus, HT (ASCP)
Laboratory Manager

PathGroup
4071 S. Access Rd, Suite 107
Chattanooga, TN 37406
423-493-0207
423-493-0208 fax
mlas...@pathgroup.com

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Re: [Histonet] Histonet Digest, Vol 157, Issue 1Glypican-3

[Steve McClain via Histonet]

Victoria Verno
You might try normal Placenta 
or fetal liver from POC 
as non-neoplastic stain controls.

That strategy may allow you enough repetitions to develop the stain until you 
find a case of HCC to test.


Steve A. McClain, MD

*

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Re: [Histonet] Competency for Supervisors

[Mighnon Lashus via Histonet]

Did anyone have a form for the Histology Supervisor Competency?

Thanks,

Mighnon Lashus, HT (ASCP)
Laboratory Manager

PathGroup
4071 S. Access Rd, Suite 107
Chattanooga, TN 37406
423-493-0207
423-493-0208 fax
mlas...@pathgroup.com

Important Notice: This e-mail is intended for the use of the person to whom it 
is addressed and may contain information that is privileged and confidential. 
If you are not the intended recipient, any disclosure, copying, distribution, 
or use of the contents of this message is strictly prohibited. If you have 
received this e-mail in error, please destroy this message and contact the 
Security Officer at PathGroup immediately at 615-562-9255. Thank you
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Re: [Histonet] Histonet Digest, Vol 157, Issue 1

[WILLIAM JACKSON via Histonet]

Can someone please give me some tips in using an automatic microtome wheel, 
were the advance wheel keeps movivg eith forward of backward until you take 
your hand off of it, any little tip is useful. Meyosha Jackson HT(ASCP)

> 
> On December 1, 2016 at 1:00 PM histonet-requ...@lists.utsouthwestern.edu 
> wrote:
> 
> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
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> To subscribe or unsubscribe via the World Wide Web, visit
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> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> Today's Topics:
> 
>1. Re: Uneven ER/PR (Terri Braud)
>2. HCC Control Tissue (Verno, Victoria R.)
>3. Reticulin stain for poorly processed tissue (sharon Celligent)
>4. Histology Openings Nationwide (Angie Laparidis)
> 
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> On November 30, 2016 at 1:38 PM Terri Braud  
> wrote:
> 
> My first 2 items to check whenever I have uneven IHC staining are (1) 
> Inadequate deparaffinization, (2) bad lot of charged slides - yes this can 
> cause terribly streaky or spotty staining, and since you are using the Bond, 
> perhaps there was an issue with coverplate placements.
> Just things to consider. Hope this helps, Terri
> 
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> 
> Today's Topics:
> 
>1. ER/PR Uneven Staining (Joanne Clark)
>   Message: 2
>   Date: Tue, 29 Nov 2016 21:09:17 +
>   From: Joanne Clark 
>   Subject: [Histonet] ER/PR Uneven Staining
>   I had a breast needle core today that when stained with ER and PR 
> the staining was uneven throughout the core, even though the cancer cells 
> were present in the entire core. The specimen had 10 hours fixation in 10% 
> NBF. I could understand the uneven staining from inadequate fixation on large 
> grossed in breast tissue, but 10 hours with needle core biopsies has always 
> been more than sufficient. Does anyone have any ideas? We use Leica's ER and 
> PR antibodies on the BOND.
>   Joanne Clark, BAAS, HT(ASCP)CM
>   Director of Histology
> 
> On December 1, 2016 at 11:31 AM "Verno, Victoria R." 
>  wrote:
> 
> Hey anyone out there have any HCC control tissue? We are looking for 
> positive liver control tissue to validate Glypican-3. Thank you for your 
> assistance.
> 
> Victoria Verno
> 
> Maryview Histology Supervisor
> 
> Maryview Medical Center
> 
> 3636 High Street
> 
> Portsmouth, VA 23707
> 
> 757-398-4763
> 
> 
> 
> The information in this communication is intended to be confidential to 
> the Individual(s) and/or Entity to whom it is addressed.
> It may contain information of a Privileged and/or Confidential nature, 
> which is subject to Federal and/or State privacy regulations.
> In the event that you are not the intended recipient or the agent of the 
> intended recipient, do not copy or use the information
> contained within this communication, or allow it to be read, copied or 
> utilized in any manner, by any other person(s). Should
> this communication be received in error, please notify the sender 
> immediately either by response e-mail or by phone,
> and permanently delete the original e-mail, attachment(s), and any copies.
> 
> 
> 
> 
> On December 1, 2016 at 11:58 AM sharon Celligent 
>  wrote:
> 
> I am working on a project and have received slides containing tissue that
> seem to be poorly processed. I am currently using a Chandler's Reticulin
> stain kit that was purchased through American MasterTech. My liver control
> on the test slide is working but the tissue of unknown processing is not
> staining. The following conditions may be the problem: poor processing,
> disease process, treatment of disease.
> Thank you,
> Sharon
> 
> On December 1, 2016 at 12:53 PM Angie Laparidis  
> wrote:
> 
> Hi Histonet Members,
> 
> I hope you are doing well. I am a Recruiter at 

Re: [Histonet] (no subject)

[Blazek, Linda via Histonet]

Try the BioCare antibody.  It's excellent.

-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, December 01, 2016 1:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Since, Cell Marque H. Pylori is not available,  can some one give a comparable 
antibody to order?  Thanks in advance


Nirmala Srishan








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for patient care, clinical performance and workplace excellence.
Click here to learn more.

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[Histonet] (no subject)

[Nirmala Srishan via Histonet]

Since, Cell Marque H. Pylori is not available,  can some one give a 
comparable antibody to order?  Thanks in advance


Nirmala Srishan








Holy Name Medical Center is ranked among the top hospitals in the nation 
for patient care, clinical performance and workplace excellence.
Click here to learn more.

 Warning: The information contained in this message is privileged and 
CONFIDENTIAL and is intended only for the use of the addressee above. If 
you are not the intended recipient, you are hereby notified that any 
disclosure, copying, distribution, or taking of any action in reliance on 
the content of this message is strictly prohibited. If you have received 
this communication in error, please notify the sender by replying to this 
message, and then delete it from your system.




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Re: [Histonet] Uneven ER/PR

[Greg Dobbin via Histonet]

Joanne,
I do not think you should expect to get perfectly uniform IHC staining in
breast core biopsies every time. At our hospital, we prefer to avoid doing
hormone receptor stains on breast cores because they are not as "robust" as
a lumpectomy or mastectomy specimen.

The unevenness could be due to pre-analytic causes beyond your control
(such as: are they placed in directly and immediately into formalin by the
radiologist? Is your processing schedule optimized for small biopsies or is
it a "one-size fits all" schedule for large and small specimens, etc).
Occasionally, we will agree to an Oncology request to stain them but this
is usually only done when they feel it is not going to be clinically
beneficial to put the patient through a more invasive procedure (ie
lumpectomy).

Another disadvantage of looking at cores vs lump specimens is that you
cannot see the "bigger picture". Perhaps there is heterogeneity in the
intensity of the ER/PR expression throughout the tumor; something that is
easier to discern when you can see the staining pattern across the entire
cross-section of the tumor.

I too use the Bond platform. If you are placing appropriate tissue controls
on the same slide as the patient and these have the expected staining
pattern and intensity, you may be fairly certain that the issue was (or is)
pre-analytic in nature. If this is not your practice, then try re-staining
new sections of the same case. If it is corrected, contact Leica Tech
support to try to figure out what went wrong the first time. If it is not
corrected, then you are back to suspecting a pre-analytic issue (assuming
you are not seeing this uneven-ness in other IHC specimens). I strongly
encourage use of tissue controls on every patient slide.

Cheers,
Greg

-- 
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*
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[Histonet] Histology Openings Nationwide

[Angie Laparidis via Histonet]

Hi Histonet Members,


I hope you are doing well. I am a Recruiter at a highly successful and well
respected Healthcare recruiting firm. I help place Histology Professionals
in permanent positions across the country and I wanted to see if you or
someone you know may be interested in exploring other career opportunities?
We are completely free of charge to candidates and and we work on quite a
few laboratory openings across the country. Our clients typically assist
with relocation expenses.

Below is a list of some of the other opportunities we are currently working
on. If you do not see an opening in a location in which you live or would
like to live, please send me an email me a copy of your resume and let me
know where you would be interested in a job. I will then tailor a search
for you that is completely confidential and free to candidates.

Histology Job Opportunities (HT or HTL):

AR-Little Rock-Histotech (1st shift 4:30/5 am start)
FL-Tampa-HLA Supervisor
IL-Chicago-Histotechnician
ME-Southern/coastal-Histotech
NC-Raleigh-IHC Tech
NC-Raleigh-Histotech
ND-Fargo-Pathologists Assistant
NM-Southern-Assistant Histology Supervisor
NY-Long Island-Histotech  (NYS license required)
NY-Long Island-Histology Supervisor
MO-St Louis-Histotech (3rd shift)
OH-Columbus-Histotech
TN-Nashville-Histotech
VA-Richmond-Histotechnologist  (days)
VA-Roanoke-Histotech
WI-Green Bay-HT/ HTL (2nd & 3rd shifts)



If you're interested in learning more about these opportunities or
opportunities in a certain geographic location please reply with an updated
resume and let me know when a good time to reach you is.


If this is not the right fit for you please let me know who you can
recommend and give me an idea of what types of positions you'd be
interested in hearing about in the future. I cover the entire US and have
am working on Lab positions at all levels. We offer a very generous
referral bonus for anyone you refer to us that we place into any position
across the country.

To view some additional opportunities please visit our website at
www.ka-recruiting.com.


Sincerely,



*Angie Laparidis*Healthcare Recruiter
K.A. Recruiting, Inc.
10 Post Office Square, 8th Floor South, Boston, MA, 02109
W:  617.746-2744 (*please note this is a new number*)
F: (617) 507-8009
an...@ka-recruiting.com


Our openings are updated daily at www.ka-recruiting.com
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[Histonet] Reticulin stain for poorly processed tissue

[sharon Celligent via Histonet]

I am working on a project and have received slides containing tissue that
seem to be poorly processed. I am currently using a Chandler's Reticulin
stain kit that was purchased through American MasterTech. My liver control
on the test slide is working but the tissue of unknown processing is not
staining. The following conditions may be the problem: poor processing,
disease process, treatment of disease.
Thank you,
Sharon
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[Histonet] HCC Control Tissue

[Verno, Victoria R. via Histonet]

Hey anyone out there have any HCC control tissue?  We are looking for positive 
liver control tissue to validate Glypican-3. Thank you for your assistance.



Victoria Verno



Maryview Histology Supervisor

Maryview Medical Center

3636 High Street

Portsmouth, VA 23707

757-398-4763




The information in this communication is intended to be confidential to the 
Individual(s) and/or Entity to whom it is addressed.
It may contain information of a Privileged and/or Confidential nature, which is 
subject to Federal and/or State privacy regulations.
In the event that you are not the intended recipient or the agent of the 
intended recipient, do not copy or use the information
contained within this communication, or allow it to be read, copied or utilized 
in any manner, by any other person(s). Should
this communication be received in error, please notify the sender immediately 
either by response e-mail or by phone,
and permanently delete the original e-mail, attachment(s), and any copies.

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