Re: [Histonet] Coverslipping

[Ifeoluwa Ajayi via Histonet]

After cover slipping we just allow the slide to stay on the bench for it to
dry. We then carefully remove the extra mountant with the aid of scalpel
blade. Of course dust may cover it but cotton wool and xylene this can be
removed.

Ajayi Ifeoluwa BMLS, MSc
University College Hospital UCH
Ibadan, Nigeria.

On Dec 21, 2016 10:31 PM, "Jessica Riggleman via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> Hello,
>
> Would anyone mind sharing their procedure after coverslipping a slide?
>
> Do you let the slide sit and dry? Do you use xylenes to wipe of the extra
> mounting medium immediately?
>
> I noticed after storing the slides in a slide box, a lot of dust collects.
> Is this because of the xylenes? Should I wipe the slide with alcohol or
> wrap them to prevent this? What do you use to wrap your slides?
>
> Please keep in mind they are large, 2x3 slides.
>
> Thank you for all of your input,
>
> Best,
> Jessica Riggleman
>
>
> _
>
> Jessica Riggleman | Research Associate
>
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
>
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[Histonet] Coverslipping

[Jessica Riggleman via Histonet]

Hello,

Would anyone mind sharing their procedure after coverslipping a slide?

Do you let the slide sit and dry? Do you use xylenes to wipe of the extra 
mounting medium immediately?

I noticed after storing the slides in a slide box, a lot of dust collects. Is 
this because of the xylenes? Should I wipe the slide with alcohol or wrap them 
to prevent this? What do you use to wrap your slides?

Please keep in mind they are large, 2x3 slides.

Thank you for all of your input,

Best,
Jessica Riggleman


_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the 
use of the individual to whom it is addressed. If you are not the intended 
recipient, be advised that you have received this email in error and that any 
use, dissemination, forwarding, printing, or copying of this email is strictly 
prohibited. If you have received this email in error please contact the sender. 
Any views or opinions presented are solely those of the author and do not 
necessarily represent those of Globus Medical, Inc. Although this email and any 
attachments are believed to be free of any virus or other defects which might 
affect any computer or IT system into which they are received, no 
responsibility is accepted by Globus Medical, Inc. for any loss or damage 
arising in any way from the receipt or use thereof.


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