Or lab is currently processing a human whole brain. In about a month or two, the whole brain, which will be encased in celloidin & serial sections will be cut at 120um each. Now, we’ve bought an old Tetrander cast iron microtome. If you haven’t seen one of these microtomes, I can tell you it’s BIG!
Now, we’ll have to stain quite a number of these sections for IHC. In fact too many to handle manually. If possible, need to find a way to at least stain the majority of these sections in a semi-automatic system e.g. washes, quenching & blocking. Does any one think it’s possible to convert a LABGO processor (made in India & can hold 2 liter glass beakers) or a Sakura-Tissue-Tek 4640 (also holds glass beakers - both are old style circular processors using glass beakers for staining these sections? Does anyone have an alternative system? I could sure use some input or ideas anyone welcome and most appreciated. Maria Mejia Lead Histologist UCSF Mission Bay San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
