Re: [Histonet] Tissue Fixation

[Tony Henwood (SCHN) via Histonet]

If the specimen is placed on absorbent paper prior to fixation, the paper will 
gradually absorb the water from the specimen causing it to dry out.
Microscopically the tissue will look  a little like what you see when a laser 
scalpel is used to excise a skin specimen. The heat affected eosin stained 
collagen will look quite brilliant (even fluorescent like) and if you do a 
Masson stain for connective tissue will give a red colouration, rather than a 
green or blue you would normally see with well-fixed collagen.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: T H via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, 31 March 2017 11:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Fixation

Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.  That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?  Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim

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Re: [Histonet] Histonet Digest, Vol 161, Issue 2, "SURVEY"

[Brent Adams via Histonet]

In our GI Laboratory we have both and we do between 35 and 55 blocks per day. I 
would not want to have to choose between the both.



I would get a "new" Auto stainer because the quality of your stained slides 
would be more consistent and be easier to assess for QC purposes.



I would get a "used" Tissue-Tek coverslip machine because it is a work horse 
tank and rarely has problems and would increase productivity by 5x over doing 
hand coverslips as well as reduce the chemical hazards exposure to the Tech.


Brent Adams – BS, LPN, HT


www.acadianagastro.com

Acadiana Gastroenterology Associates, LLC
439 Heymann Blvd
Lafayette, LA  70503

tel:  (337) 269-1126
fax:  (337) 269-1476



From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Sunday, April 2, 2017 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 161, Issue 2

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Today's Topics:

   1. Re: Survey!! (Patsy Ruegg)
   2. Re: Survey!! (Rene J Buesa)


--

Message: 1
Date: Sat, 1 Apr 2017 23:18:49 +
From: Patsy Ruegg 
To: Ifeoluwa Ajayi , "Patti Nelson - PNP Lab
Consultant" 
Cc: "Histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Survey!!
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

I have seen so many more problems with auto coverslippers, they break down, the 
cover does not hold up over time for archiving, etc., that I would would 
probably chose an autostainer.



Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com




From: Ifeoluwa Ajayi 
Sent: Saturday, April 1, 2017 6:11 AM
To: Patti Nelson - PNP Lab Consultant
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Survey!!

Personally I will prefer auto coverslipper, because cover slipping is
cumbersome and takes time.

Ajayi Ifeoluwa, BMLS, AMLSCN, MSc,
UCH Ibadan, Nigeria.

On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

>
> Hi Everyone,
>
> I just wanted to get everyone's opinion. If you had to chose between
> buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you
> chose? Lets say your volume was around 60 to 80 blocks a day and you worked
> for a GI Lab. Everyone's input would be greatly appreciated.
>
>
>
> Sincerely,
>
> PATTI NELSON  H.T.(ASCP)
> PNP LABORATORY CONSULTANTS
> SUPERVISOR DGC/ZADEH LABS
> PO BOX 412
> CABAZON, CA. 92230
> 909-841-9761
> nelsonr...@verizon.net
> CONFIDENTIALITY NOTICE:This message and any included attachments are from
> Patti Nelson, PNP Laboratory Consultants and are intended only for the
> addressee. The information contained in this message is confidential and
> may contain privileged, confidential, proprietary and/or exemption from
> disclosure under applicable law.  Unauthorized forwarding, printing,
> copying, distribution, or use of such information is strictly prohibited
> and may be unlawful.  If you are not the addressee, please promptly delete
> this message and notify the sender ofthe delivery error by e-mail or you
> may call  909-841-9761.
>
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Lists
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--

Message: 2
Date: Sun, 2 Apr 2017 14:03:02 + (UTC)
From: Rene J Buesa 
To: Patsy Ruegg ,  Ifeoluwa Ajayi
,   Patti Nelson - PNP Lab Consultant

Cc: "Histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Survey!!
Message-ID: <1790187348.9333912.1491141782...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

Re: [Histonet] Survey!!!!!!

[Frazier, John via Histonet]

The one thing I would do is to make sure you choose a glass coverslip.
Plastic is not good over time and varying temperatures.

Sent from my iPad

> On Apr 1, 2017, at 7:18 PM, Patsy Ruegg  wrote:
>
> I have seen so many more problems with auto coverslippers, they break down, 
> the cover does not hold up over time for archiving, etc., that I would would 
> probably chose an autostainer.
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 40864 E Arkansas Ave
> Bennett, CO 80102
> H 303-644-4538
> C 720-281-5406
> prueg...@hotmail.com
>
>
>
> 
> From: Ifeoluwa Ajayi 
> Sent: Saturday, April 1, 2017 6:11 AM
> To: Patti Nelson - PNP Lab Consultant
> Cc: Histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Survey!!
>
> Personally I will prefer auto coverslipper, because cover slipping is
> cumbersome and takes time.
>
> Ajayi Ifeoluwa, BMLS, AMLSCN, MSc,
> UCH Ibadan, Nigeria.
>
> On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via Histonet" <
> histonet@lists.utsouthwestern.edu> wrote:
>
>>
>> Hi Everyone,
>>
>> I just wanted to get everyone's opinion. If you had to chose between
>> buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you
>> chose? Lets say your volume was around 60 to 80 blocks a day and you worked
>> for a GI Lab. Everyone's input would be greatly appreciated.
>>
>>
>>
>> Sincerely,
>>
>> PATTI NELSON  H.T.(ASCP)
>> PNP LABORATORY CONSULTANTS
>> SUPERVISOR DGC/ZADEH LABS
>> PO BOX 412
>> CABAZON, CA. 92230
>> 909-841-9761
>> nelsonr...@verizon.net
>> CONFIDENTIALITY NOTICE:This message and any included attachments are from
>> Patti Nelson, PNP Laboratory Consultants and are intended only for the
>> addressee. The information contained in this message is confidential and
>> may contain privileged, confidential, proprietary and/or exemption from
>> disclosure under applicable law.  Unauthorized forwarding, printing,
>> copying, distribution, or use of such information is strictly prohibited
>> and may be unlawful.  If you are not the addressee, please promptly delete
>> this message and notify the sender ofthe delivery error by e-mail or you
>> may call  909-841-9761.
>>
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Histonet Info Page - lists.utsouthwestern.edu Mailing 
> Lists
> lists.utsouthwestern.edu
> Histonet -- For the exchange of information pertaining to histotechnology and 
> related fields About Histonet
>
>
>>
>
>

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Re: [Histonet] Survey!!!!!!

[Rene J Buesa via Histonet]

Staining is a more complex step than coverslipping. Also a coverslip on a 
section has more "latitude" and can, if necessary, be removed and coverslip in 
a different way. A stained section, on the other hand, is almost impossible to 
"correct". Between an autostainer and a coverslipper, I would choose an 
autostainer.René 

On Saturday, April 1, 2017 7:29 PM, Patsy Ruegg via Histonet 
 wrote:
 

 I have seen so many more problems with auto coverslippers, they break down, 
the cover does not hold up over time for archiving, etc., that I would would 
probably chose an autostainer.



Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com




From: Ifeoluwa Ajayi 
Sent: Saturday, April 1, 2017 6:11 AM
To: Patti Nelson - PNP Lab Consultant
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Survey!!

Personally I will prefer auto coverslipper, because cover slipping is
cumbersome and takes time.

Ajayi Ifeoluwa, BMLS, AMLSCN, MSc,
UCH Ibadan, Nigeria.

On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

>
> Hi Everyone,
>
> I just wanted to get everyone's opinion. If you had to chose between
> buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you
> chose? Lets say your volume was around 60 to 80 blocks a day and you worked
> for a GI Lab. Everyone's input would be greatly appreciated.
>
>
>
> Sincerely,
>
> PATTI NELSON  H.T.(ASCP)
> PNP LABORATORY CONSULTANTS
> SUPERVISOR DGC/ZADEH LABS
> PO BOX 412
> CABAZON, CA. 92230
> 909-841-9761
> nelsonr...@verizon.net
> CONFIDENTIALITY NOTICE:This message and any included attachments are from
> Patti Nelson, PNP Laboratory Consultants and are intended only for the
> addressee. The information contained in this message is confidential and
> may contain privileged, confidential, proprietary and/or exemption from
> disclosure under applicable law.  Unauthorized forwarding, printing,
> copying, distribution, or use of such information is strictly prohibited
> and may be unlawful.  If you are not the addressee, please promptly delete
> this message and notify the sender ofthe delivery error by e-mail or you
> may call  909-841-9761.
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Histonet Info Page - lists.utsouthwestern.edu Mailing 
Lists
lists.utsouthwestern.edu
Histonet -- For the exchange of information pertaining to histotechnology and 
related fields About Histonet


>

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