Re: [Histonet] Fabric softener to "decalcify" bone? - Downy has formic acid??

[Angela Lamberth via Histonet]

Just googled the ingredients and found this: https://imgur.com/a/Y5170

On Thursday, April 13, 2017, Angela Lamberth  wrote:

> Hi netters!
>
> A friend sent me the link below and we are curious to hear your thoughts
> and experiences with using fabric softener (in lieu of standard decal
> methods) on bone before processing.
>
> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309838/
>
> Best,
> Angela
>
> --
> Angela Lamberth
> Histology Technician III
> Histology Core Lab
> La Jolla Institute for Allergy & Immunology
> 9420 Athena Circle
> La Jolla, CA 92037
>


-- 
Angela Lamberth
Histology Technician III
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
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[Histonet] Fabric softener to "decalcify" bone?

[Angela Lamberth via Histonet]

Hi netters!

A friend sent me the link below and we are curious to hear your thoughts
and experiences with using fabric softener (in lieu of standard decal
methods) on bone before processing.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309838/

Best,
Angela

-- 
Angela Lamberth
Histology Technician III
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
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Re: [Histonet] Fw: orders from resident surgeons

[Rene J Buesa via Histonet]

At my hospital we accepted work orders from our pathologists only. If a 
physician resident wanted some test, it has to be approved by our 
pathologist.René 

On Thursday, April 13, 2017 12:18 PM, "Horn, Hazel V via Histonet" 
 wrote:
 

 
I was not clear enough in my question.  I'm not speaking of pathology residents 
ordering tests.  I'm asking if you allow general resident physicians to order 
pathology tests.  Such as gross and micro.

We are a teaching hospital and have never allowed a resident to order pathology 
if they aren't a staff physician (surgeon).

Thanks!



From: Horn, Hazel V
Sent: Thursday, April 13, 2017 8:17 AM
To: histonet
Subject: orders


In the 25+ years I have worked at ACH we have never allowed a resident 
physician to order a pathology test.  I am now being asked why we do this?  I 
have no idea if there is a rule from Medicaid or insurance providers that all 
orders must be from a staff physician.  Does your hospital allow residents to 
place pathology orders?



Hazel Horn

Supervisor of Histology/Autopsy/Transcription

Anatomic Pathology

Arkansas Children's Hospital

1 Children’s Way | Slot 820| Little Rock, AR 72202

501.364.4240 direct | 501.364.1241 fax

hor...@archildrens.org

archildrens.org

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[Histonet] RELIA Special Histology Alert - Strike While the Iron's Hot! and Advance in leadership!! 4-13-2017

[Pam Barker via Histonet]

Hi Histonetters,
How are you?  I hope you are having a great week!!

Are you ready for the next step in your career?
Histo tech to lead tech or Lead Tech to Supervisor??
Supervisor to Manager?

These days you have to strike when the iron’s HOT!
I am working with several of my best clients who are offering the rare and
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Are you someone who:
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2. Doesn’t see a path to management in your current job
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I am currently working with several top employers who want to hire someone
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These opportunities don’t come along every day!
Here are the locations:
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My clients offer excellent compensation, benefits, relocation assistance and
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These are permanent full time positions in busy growing private labs.  

The help I need is do you know anyone that might be interested in hearing
about any of these opportunities?  If so could you please forward my e-mail
to them?

 If you are interested in any of these positions please call me ASAP at
866-607-3542 or on my cell/text at 407-353-5070 or e-mail me at
rel...@earthlink.net  


Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
https://www.facebook.com/RELIASolutionsforhistologyprofessionals
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 
  


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Re: [Histonet] help!!

[Caroline Miller via Histonet]

Blanca,
Here are my feelings on this, and I am sure a lot of other folks have feels
here too, so please chime in.

1 - I feel that most clinical labs are more on the IHC bandwagon and
research labs are IF (with the exception of IgG staining in kidney biopsies
or bullous disease in skin- which is because the antibodies don't like
formalin fixing (if this is now wrong I am sorry, I haven't been in a
clinical lab in quite a while). Research labs are often also working with
genetically encoded fluorophores such as GFP, YFP, mCherry
2 - Formalin fixation (especially over fixation) can often lead to a large
amount of autofluorescence in the 488 region, which is a common place for
secondary antibodies and also GFP. Research labs have a lot more control
over their fixation protocols.
3 - The microscopes commonly available to clinical labs are bright field
scopes and in research labs fluorescent scopes
4 - Fluorescence can provide more contrast to a positively localized
fluorophore, but sometimes at the detriment of viewing the overall
morphology of the tissue like you get with bright field IHC and a nuclear
counterstain.
5 - Research lab protocols are often very 'experimental' and can lead to
increased tissue damage, which again is not viewed under the fluorescence
microscope (as much). Clinical labs have lots of experience and also
defined protocols that work well in the IHC / bright field space.
6 - the only real difference is the detection method, you can use any
primary antibody with either ABC/ impress / enzyme based methods or with
fluorophore conjugated secondaries.

So, in short - no *real* reason, but mainly that is the way things shook
out.

I could go on about researchers not understanding how to take photos on a
bright field scopes too, but that is too broad a statement, but as a core
director I saw them being more comfortable with the fluorescent methods :)

mills




On Thu, Apr 13, 2017 at 6:09 AM, Blanca Lopez via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello!
> I just need a help with a simple question...Is anyone can explain me what
> is the purpose between performing immunohistochemistry and
> Immunofluorescence?
> Thanks  :)
>
> Blanca Lopez
> Histotech (ASCP)
> UTSW Tissue Resource K1.210
> Simmons Comprehensive Cancer Center
> UT Southwestern Medical Center
> Telephone: 214-648-7598
> Email: blanca.lo...@utsouthwestern.edu
>
>
> 
>
> UT Southwestern
>
>
> Medical Center
>
>
>
> The future of medicine, today.
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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[Histonet] Fw: orders from resident surgeons

[Horn, Hazel V via Histonet]

I was not clear enough in my question.  I'm not speaking of pathology residents 
ordering tests.  I'm asking if you allow general resident physicians to order 
pathology tests.  Such as gross and micro.

We are a teaching hospital and have never allowed a resident to order pathology 
if they aren't a staff physician (surgeon).

Thanks!



From: Horn, Hazel V
Sent: Thursday, April 13, 2017 8:17 AM
To: histonet
Subject: orders


In the 25+ years I have worked at ACH we have never allowed a resident 
physician to order a pathology test.  I am now being asked why we do this?   I 
have no idea if there is a rule from Medicaid or insurance providers that all 
orders must be from a staff physician.   Does your hospital allow residents to 
place pathology orders?



Hazel Horn

Supervisor of Histology/Autopsy/Transcription

Anatomic Pathology

Arkansas Children's Hospital

1 Children’s Way | Slot 820| Little Rock, AR 72202

501.364.4240 direct | 501.364.1241 fax

hor...@archildrens.org

archildrens.org

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Re: [Histonet] help!!

[Morken, Timothy via Histonet]

Blanca, immunofluorescence (IF) is a subset of immunochemistry. 
Immunohistochemistry is also a subset of immunochemistry. There is some overlap 
between the two.

Immunohistochemistry denotes  immunochemistry done on tissue sections 
("-histo-" =" tissue"). But we can also use other enzymes to label the 
antibodies for immunohistochemistry (peroxidase, alkaline phosphatase, etc).


IF is just one of many methods of labeling the antibodies with a visual label. 
Others are peroxidase and alkaline phosphatase.


Generally IF is done on "fresh" cells or tissue. For tissue it is normally 
frozen tissue. 

IF can be done on cells (ie, immunocytochemistry) either on slides (smears, 
various preparations) or in solution as with flow cytometry - the cells are 
labeled with fluorescent-labeled antibodies and sorted by color (or no color). 

Generally the IF method is faster to perform because there is no processing 
beyond freezing the tissue. In the past IF was also more sensitive due to dark 
field microscopy in the fluorescence microscope. With the advent of various 
methods to amplify the signal (avidin -biotin, polymers with multiple enzymes) 
the peroxidase methods are just as sensitive, if not more so.

But fresh or frozen tissue has the advantage of the epitopes remaining unfixed, 
especially by formalin - which can mask the antigen from the antibody. Some 
antibodies do not work well on formalin-fixed tissue, even if antigen retrieval 
is used, so frozen tissue or cells are the best option. 



Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center





-Original Message-
From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, April 13, 2017 6:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] help!!

Hello!
I just need a help with a simple question...Is anyone can explain me what is 
the purpose between performing immunohistochemistry and Immunofluorescence?
Thanks  :)

Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu




UT Southwestern


Medical Center



The future of medicine, today.

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[Histonet] Testicular biopsy processing

[Lester Raff MD via Histonet]

Hello all-although we are a Uropath lab, up until now we have not processed 
testicular biopsies, and it has been years since I have done so. Do most labs 
still use Bouin's Solution as the fixative of choice? Any precautions or 
special handling techniques?

Thanks for any and all input.
--
Lester J. Raff, MD MBA
UroPartners

Latest blog: 
http://www.chicagonow.com/downsize-maybe/2017/04/learning-to-be-a-patient/

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[Histonet] help!!

[Blanca Lopez via Histonet]

Hello!
I just need a help with a simple question...Is anyone can explain me what is 
the purpose between performing immunohistochemistry and Immunofluorescence?
Thanks  :)

Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu




UT Southwestern


Medical Center



The future of medicine, today.

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[Histonet] orders

[Horn, Hazel V via Histonet]

In the 25+ years I have worked at ACH we have never allowed a resident 
physician to order a pathology test.  I am now being asked why we do this?   I 
have no idea if there is a rule from Medicaid or insurance providers that all 
orders must be from a staff physician.   Does your hospital allow residents to 
place pathology orders?



Hazel Horn

Supervisor of Histology/Autopsy/Transcription

Anatomic Pathology

Arkansas Children's Hospital

1 Children’s Way | Slot 820| Little Rock, AR 72202

501.364.4240 direct | 501.364.1241 fax

hor...@archildrens.org

archildrens.org

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