[Histonet] CE opportunities

[Jennifer MacDonald via Histonet]

   The California Society for Histotechnology is hosting their annual
   symposium May 18-21 in San Jose.  Please join us for some great
   workshops and California spring weather.  San Jose is a great airport
   to fly into.

   More information can be found at:
   http://californiahistology.org/events.html
   The deadline for CSH hotel pricing is April 28.
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Re: [Histonet] help!!

[Tony Henwood (SCHN) via Histonet]

Hi Bianca,
Well for most Pathology departments, Immunofluorescence (IF) is used for Renal 
and Skin biopsies; looking for human Immunoglobulin (Igs) deposition on 
basement membranes. The advantage here is not so much the fluorescence, but 
that we use unfixed frozen sections. The buffer rinse before antibody 
application, removes un-bound serum immunoglobulins, leaving any pathological 
bound Igs for the IF antibody to bind to. This gives a clean result.

If one would do IF on formalin-fixed paraffin sections of renal or skin 
biopsies, you would find heavy background due to the fixative cross-linking 
serum Igs to tissue and cells (which would usually be removed by the buffer 
rinse if unfixed frozen sections were used - see above).

IF, apart from being a historic method, also does not suffer from endogenous 
peroxidase that would need to be blocked if peroxidase was used in place of 
fluorescence.



Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, 13 April 2017 11:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] help!!

Hello!
I just need a help with a simple question...Is anyone can explain me what is 
the purpose between performing immunohistochemistry and Immunofluorescence?
Thanks  :)

Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu




UT Southwestern


Medical Center



The future of medicine, today.

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[Histonet] Texas Society for Histotechnology Annual Symposium May 5-7, 2017

[Shelly Coker via Histonet]

Hello Histonetters!
I wanted to share an opportunity to earn some valuable CEU's while networking 
and enjoying the company of your peers.  We are having our 39th Annual Texas 
Society for Histotechnology Symposium ad Convention.  We have a wide variety of 
workshops available from basic skills to advanced IHC techniques.  Join us May 
5-7 at the Austin Renaissance and connect with fellow histology professionals.  
Visit the following link for hotel and class information  The Texas Society of 
Histotechnology .
  
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The Texas Society of Histotechnology
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[Histonet] Tissue Disposal Procedure:

[J B via Histonet]

Does anyone have a tissue disposal procedure that you can share?  How do
you log, track, etc specimens that have been disposed?

Thank you,

JB
-- 

Have a great day!
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[Histonet] CAP regulations regarding storage of aliquots of concentrated antibodies

[Adesupo, Adesuyi (Banjo) via Histonet]

Hi,
I will like to know the CAP regulations regarding storage of aliquots of 
concentrated antibodies in refrigerator freezers.

 Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS,
 Histology Supervisor
 Norman Regional Health System,
 Norman, OK 73071.
 Tel: 405- 307- 1145
abades...@nrh-ok.com

==
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Re: [Histonet] Hirsch-Pfeiffer cresyl violet method

[Morken, Timothy via Histonet]

Bob, Thanks for the offer! I finally found a copy and Google Translate did a 
pretty good job on it, and we have a person in the lab who, it turns out, can 
read german, so I think we are set.

As an aside, the "clues" I got from tidbits of the procedure mentioned in other 
papers turned out to have little basis in reality to the original method 
described in the paper (concentrations, times, temperatures all different!), so 
I'm glad I found the original.


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Saturday, April 15, 2017 11:46 AM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Hirsch-Pfeiffer cresyl violet method

Tim Morken in pathology at UC San Francisco Medical Center asks about the 
Hirsch-Pfeiffer (correct spelling) cresyl violet stain for frozen sections.

"I've found many references to it, but none that give the procedure. And the 
original paper is from 1955 not easily available. And is in German."

If you can get the paper, I can read it for you.

Bob Richmond
Samurai Pathologist
Maryville TN
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