[Histonet] (no subject)

[Steve McClain via Histonet]

If it is any help, A dollar bill weighs 1 gram and a quarter weighs 5.67 grams. 
It is simpler to write up some calibration procedure based on knowns. 

Steve A. McClain, MD

> On Jun 8, 2017, at 13:20, "histonet-requ...@lists.utsouthwestern.edu" 
>  wrote:
> 
> Balance calibration
> Message-ID:

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[Histonet] plastic section gram stain

[Lauren Sweeney via Histonet]

Hello histonet world,

My pathologist just gave me a slide with a section on it that is intended for 
electron microscopy. Has if anyone ever done a Brown and Hopps special on a 
section embedded in Epon/Araldite?  She told me that her colleague has had the 
sections stained successfully with T-Blue on a hot plate before. But gram 
stains are a bit more complicated than a T-Blue! I can heat the staining 
components (i.e. crystal violet, safranin o, and iodine) but there are removal 
steps that require use of acetone, formaldehyde, acetic acid, and picric acid, 
and I am not sure how those reagents will work with the plastic. Any help or 
experience to share would be wonderful.

Thanks,
Lauren

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[Histonet] Question regarding frozen control tissue

[Haley Huggins via Histonet]

I am trying to find a way to obtain small bowel/intestine as a fresh/frozen
sample to be able to use it for a control with my staining for PGP9.5 free
floating IHC stain. Does anyone know where a good place would be to obtain
the needed control tissue?

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, *
*Templeton, CA 93465*
*Office: 877-230-1518*
*Cell: 303-652-7453*
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