[Histonet] Grossing Blade for Ophthalmology Globes

[Sandra Cheasty via Histonet]

Hello all,
Can someone recommend an economical disposable blade for 
grossing eye globes? Sharp enough to slice through sclera smoothly, strong 
enough to finish the cut through the lens.
Cheers!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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Re: [Histonet] Water Quality from Lab Gen Checklist distilled vs deionized vs reagent grade water

[Morken, Timothy via Histonet]

Renee, for practical purposes in histology they are pretty much the same. Note 
that the original water quality is key because certain things can carry over in 
each type of system. Commercial suppliers do all the filtering, conditioning 
and finishing for you. 

Deionized (DI) is now the usual water produced in institutions because it is 
easier to maintain and produces adequate amounts of water. Distillation 
requires a still and produces relatively small quantities compared to DI.  In 
our histo lab we have a DI system, but it would be over-kill for a small lab. 
Buying distilled water will be fine. The company that makes distilled water 
does all the filtering and finishing for you so it is pure. If really concerned 
you can buy from a scientific supplier rather than a grocery store. In our EM 
lab we make up muscle histochemistry stains and  with type 1 water we buy in 1 
liter bottles. We have a DI system for everything else. 

This is a good article on the difference between DI and Dist. Water:

http://www.labmanager.com/laboratory-technology/2014/09/the-right-water#.WdZwx4TyuUk


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Renee H. Workman via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, October 05, 2017 10:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water Quality from Lab Gen Checklist distilled vs deionized 
vs reagent grade water

Going over checklist needed input on water quality.  We are a small lab and use 
distilled water purchased commercially.  We do IHC but no special stains.  We 
use the water in our water baths and for preparing IHC buffer.  Should we 
upgrade to deionized or use distilled?

Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
Richmond, VA  23235
W: 804-527-1316 | F: 804-270-0917
rhwork...@uro.com | www.uro.com





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[Histonet] Water Quality from Lab Gen Checklist distilled vs deionized vs reagent grade water

[Renee H. Workman via Histonet]

Going over checklist needed input on water quality.  We are a small lab and use 
distilled water purchased commercially.  We do IHC but no special stains.  We 
use the water in our water baths and for preparing IHC buffer.  Should we 
upgrade to deionized or use distilled?

Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
Richmond, VA  23235
W: 804-527-1316 | F: 804-270-0917
rhwork...@uro.com | www.uro.com





Disclaimer: The email and files transmitted with it are confidential and are 
intended solely for the use of the individual or entity to whom they are 
addressed. If you are not the original recipient or the person responsible for 
the delivering the email to the intended recipient, be advised that you have 
received this email in error, and that any use, dissemination, forwarding, 
printing or copying of this email is strictly prohibited. If you received this 
email in error, please delete it from your system without copying it, and 
notify the sender by reply email so that our address record can be corrected.

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Re: [Histonet] Old control blocks

[Morken, Timothy via Histonet]

Elaine, blocks last a very long time. The at the face the tissue may be 
oxidized, like a section on a slide, but the deeper tissue should be good. If 
you have several extra blocks just coat the face with paraffin to protect it.

However, the only way to be sure is to test them occasionally. If they are used 
regularly you can just note the last successful test (we keep a validation 
record of each block and note re-validation results on the form). If not used 
too often, test once a year to ensure it is good. Test the "extra 
paraffin-coated" blocks when  they are needed, not every year - maybe when the 
previous block is nearing its end test the next block in line to be sure you 
have a control available.

For the cryostat, for what part of the cryostat is the probe displaying a 
temperature?  It may not be the chamber temperature. Our cryostat display (and 
controls) are for the knife and object holder. There is no built-in display for 
the chamber. We did put in a manual-read thermometer for the chamber, and all 
show different temperatures.

The "correct" temperature is the one at which you get the best results!


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Elaine allison Hoffman via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, October 05, 2017 8:42 AM
To: Histonetters Histonet
Subject: [Histonet] Old control blocks

 Hi everyone,
Just want to put a question out there. . . . 
How long can we keep control blocks (paraffin embedded tissue) before they go 
bad?  I just went through our old control blocks in our filing container and 
some of our control blocks have dates as far back as 10 years ago.  They have 
not been refrigerated or anything but didn't know if they should be thrown out 
and start over or how long they are good for?  They are filed in alphabetical 
order, anything from amyloid controls to yeast control blocks and many others 
in-between.  Or probably should be tested to see just how positive the staining 
results turn out to be.  I was just wondering if anyone knew off the top of 
their head, lol. Another brain teaser
Our cryostat is displaying a temperature of -23 degrees but the thermometer 
sitting inside the chamber is reading -15 degrees.  The thermometer was just 
recently purchased and does not need calibrated or anything.  Our supervisor 
thought we should have a thermometer inside the cryo-chamber just to be certain 
that the digital display is accurate.  Now we have a dilemma!  Did anyone else 
experience this problem with the temperature readings with their cryostat?  Not 
really sure what to do about the different readings.  What should the correct 
temperature be anyways?
We would really appreciate any feed-back or suggestions.
Thank you,

Elaine Hoffman, HT(ASCP)
Steward Trumbull Memorial HospitalWarren, OH 
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Re: [Histonet] Old control blocks

[Liz Chlipala via Histonet]

Elaine

We have the temperature of our cryostat calibrated yearly by an outside 
calibration company.  If the thermometer you are using inside the cryostat has 
been calibrated and is traceable  I think you might have an issue with the 
temperature reading that is being displayed on your cryostat.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: Elaine allison Hoffman via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, October 5, 2017 9:42 AM
To: Histonetters Histonet 
Subject: [Histonet] Old control blocks

Hi everyone,
Just want to put a question out there. . . .
How long can we keep control blocks (paraffin embedded tissue) before they go 
bad?  I just went through our old control blocks in our filing container and 
some of our control blocks have dates as far back as 10 years ago.  They have 
not been refrigerated or anything but didn't know if they should be thrown out 
and start over or how long they are good for?  They are filed in alphabetical 
order, anything from amyloid controls to yeast control blocks and many others 
in-between.  Or probably should be tested to see just how positive the staining 
results turn out to be.  I was just wondering if anyone knew off the top of 
their head, lol.
Another brain teaser
Our cryostat is displaying a temperature of -23 degrees but the thermometer 
sitting inside the chamber is reading -15 degrees.  The thermometer was just 
recently purchased and does not need calibrated or anything.  Our supervisor 
thought we should have a thermometer inside the cryo-chamber just to be certain 
that the digital display is accurate.  Now we have a dilemma!  Did anyone else 
experience this problem with the temperature readings with their cryostat?  Not 
really sure what to do about the different readings.  What should the correct 
temperature be anyways?
We would really appreciate any feed-back or suggestions.
Thank you,

Elaine Hoffman, HT(ASCP)
Steward Trumbull Memorial HospitalWarren, OH
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[Histonet] Old control blocks

[Elaine allison Hoffman via Histonet]

 Hi everyone,
Just want to put a question out there. . . . 
How long can we keep control blocks (paraffin embedded tissue) before they go 
bad?  I just went through our old control blocks in our filing container and 
some of our control blocks have dates as far back as 10 years ago.  They have 
not been refrigerated or anything but didn't know if they should be thrown out 
and start over or how long they are good for?  They are filed in alphabetical 
order, anything from amyloid controls to yeast control blocks and many others 
in-between.  Or probably should be tested to see just how positive the staining 
results turn out to be.  I was just wondering if anyone knew off the top of 
their head, lol.  
Another brain teaser
Our cryostat is displaying a temperature of -23 degrees but the thermometer 
sitting inside the chamber is reading -15 degrees.  The thermometer was just 
recently purchased and does not need calibrated or anything.  Our supervisor 
thought we should have a thermometer inside the cryo-chamber just to be certain 
that the digital display is accurate.  Now we have a dilemma!  Did anyone else 
experience this problem with the temperature readings with their cryostat?  Not 
really sure what to do about the different readings.  What should the correct 
temperature be anyways?
We would really appreciate any feed-back or suggestions.
Thank you,

Elaine Hoffman, HT(ASCP)
Steward Trumbull Memorial HospitalWarren, OH 
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[Histonet] RELIA Special Job Alert for Managers and Supervisors 10-5-2017 Exciting opportunities Nationwide NC*FL*CA*VA*MA*AL*

[Pam Barker via Histonet]

Hello Histonetters,

I have several exciting opportunities for experienced Managers, 
 Supervisors and Lead Techs in hospital and private lab environments in
several locations throughout the US.  These are some of the premier
employers in the United States.  The positions are of course full time and
permanent.  My clients offer excellent compensation, benefits and relocation
assistance. 

Here are my leadership positions: 

AP Manager - Charlotte, NC

Mohs Histology Supervisor - Naples, FL

Histology Supervisor - Salem, VA

Histology Supervisor - Modesto, CA

Histology Supervisor - Boston, MA

Histology Supervisor - Birmingham, AL

 

I anticipate multiple management openings with many exciting opportunities
for talented managers and supervisor to be coming available nationwide as
many of my clients are experiencing rapid growth again.

If you are interested in looking into making a job change please let me
know.  That way I can keep you apprised of management opportunities that
would interest you.  



Also if you would like more information or know of someone else who might be
interested, please contact me via email at: rel...@earthlink.net or  toll
free at: 866-607-3542 

or on my cell at: 407-353-5070.
I am available to discuss the opportunity at your convenience including
after hours. Thanks-Pam

 

There are a lot of recruiters out there right now trying to work with
histology professionals and I appreciate your support and respect your
needs.  Remember I offer over 25 years of experience as a recruiter and for
over 15 years I have dedicated my practice solely to placing histology
professionals like you.  

 

Thanks-Pam

 

Right Place, Right Time, Right Move with RELIA!

 

Thank You!
 Pam M. Barker

 

Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 

 

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[Histonet] Expired antibodies

[Charles Riley via Histonet]

Does anyone know any place that will buy or I could donate our expired
antibodies?  I just went through our refridgerator and cataloged all of the
antibodies that have expired. We were using some for teaching purposes with
the students who come through on rotations however due to budget needs we
can no longer afford to allow them to run tests. Any suggestions would be
greatly appreciated.

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] FASEB releases report based on results from the Shared Research Resources Survey

[Chiriboga, Luis via Histonet]

Dear Participants in FASEB's Shared Research Resources Survey,



Today the Federation of American Societies for Experimental Biology (FASEB) 
released the report "Maximizing Shared Research 
Resources,"
 which identifies the challenges encountered by shared research resources 
providers and users, and makes recommendations for improvement. Thank you again 
for participating in FASEB's Shared Research Resources Survey - your responses 
greatly informed this report.



The survey results demonstrated the many benefits of shared resources, from 
cost efficiency to greater access to advanced technologies and services. 
However, respondents also described the many ways shared resources can fall 
short of their full potential. Based on the survey findings, FASEB identified 
four key areas for improvement:

* Funding and Business Operations

* Discoverability and Access;

* Ability to Meet Evolving Needs;

* Facility Career Track and Staff Development.

In the accompanying recommendations, FASEB describes actions stakeholders - 
from sponsors to institutions, facilities, investigators, and scientific 
societies - can take to improve the deployment, use, and sustainability of 
shared resources.  The full report, including the recommendations, survey 
analysis, and appendices (containing the summary results and additional 
resources) can be downloaded from this 
page.



Please help us share this report! A communications toolkit can be downloaded 
here
 and provides summary text and sample social media posts. Please contact me if 
you have any questions or would like additional information.



Thank you again for participating in FASEB's Shared Research Resources Survey!

Bethany



Bethany Drehman, PhD
Senior Science Policy Analyst
Office of Public Affairs
Federation of American Societies for Experimental Biology
9650 Rockville Pike, Bethesda, MD 20814-3998
Email: bdreh...@faseb.org

Phone: (301) 634-7659


Luis Chiriboga Ph.D, IHC Director
DART Experimental Pathology
NYU School of Medicine
MSB 379
Office: 646-501-6934
Mobile: (347) 712-0897
luis.chirib...@nyumc.org



This email message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain information that is proprietary, 
confidential, and exempt from disclosure under applicable law. Any unauthorized 
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=
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