Re: [Histonet] Elastic stain

[Tony Henwood (SCHN) via Histonet]

I would recommend the Orcein stain:

Henwood, A., (2003) "Current applications of orcein in histochemistry. A brief 
review with some new observations concerning influence of dye batch variation 
and aging of dye solutions on staining" Biotech Histochem. 78(6):303-8.

And you can pair the orcein stain with several counterstains (Picro light 
green, Periodic acid Blue Schiff's, Perls, H etc.):

Henwood, A.F., (2002) "The Orcein Stain: A versatile stain for Histopathology" 
J. Histotechn. 25(1):29-32.


Regards 

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 



-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, 7 November 2017 3:13 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Elastic stain

Histonetters,

Is there someone who can recommend a Elastic stain other than Verhoeff?

Thanks
Mala









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[Histonet] Amyloid A controls

[Dooley, Elaine via Histonet]

Hello Histonetters,

I am looking for a good "Amyloid A control".  We have an anti-amyloid A 
antibody which is specific only for amyloid A.  We have tried our commercial 
congo red controls and they do not work.  We have only had a few positive 
cases.  I am willing to trade control blocks.  We have parvovirus, h,pylori,  
C4d on heart, and many other controls just ask and maybe we could make a trade.

Elaine Dooley

Shands Labs at Rocky Point
4800 SW 35th drive
Room 1130 Histology
Gainesville FL 32608

352-265-0111   ext 72117


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Re: [Histonet] Elastic stain

[Bryan Llewellyn via Histonet]

Please read the article at 
:http://stainsfile.info/StainsFile/stain/elastic/elastic.htm
There are numerous alternatives discussed there. I particularly like the 
Humberstone variant of iron resorcin fuchsin.


Bryan Llewellyn



Nirmala Srishan via Histonet wrote:

Histonetters,

Is there someone who can recommend a Elastic stain other than Verhoeff?

Thanks
Mala









Holy Name Medical Center is ranked among the top hospitals in the nation
for patient care, clinical performance and workplace excellence.
Click here to learn more.

 Warning: The information contained in this message is privileged and
CONFIDENTIAL and is intended only for the use of the addressee above. If
you are not the intended recipient, you are hereby notified that any
disclosure, copying, distribution, or taking of any action in reliance on
the content of this message is strictly prohibited. If you have received
this communication in error, please notify the sender by replying to this
message, and then delete it from your system.




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Re: [Histonet] Elastic stain

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Dear Mama,

there are several elastic stains which are as brilliant as Verhoeff.
For a very quick and easy stain you can try:

Van Gieson with Resorcinfuchsin or
Resorcinfuchsin with Thiazin-picric-acid

Also very interesting results can be generated with:

Aldehydfuchsin-Tripple Stain
and
Mollier-Staining with Orcein

You should find the protocols in standard literature like Bancroft or Romeis, 
but please allow that I insert some links to our webshop where you can buy 
these staining-kits ready-to-use:

https://webshop.morphisto.de/faerbekit-elastica-nach-van-gieson.html
https://webshop.morphisto.de/faerbekit-resorcinfuchsin-mit-thiazinrot-pikrinsaeure.html
https://webshop.morphisto.de/faerbekit-aldehydfuchsin-dreifachfaerbung.html 

https://webshop.morphisto.de/faerbekit-vierfachfaerbung-nach-mollier.html

If you have any further questions, please just contact us via email.
With best regards
Michael



> Am 06.11.2017 um 17:12 schrieb Nirmala Srishan via Histonet 
> :
> 
> Histonetters,
> 
> Is there someone who can recommend a Elastic stain other than Verhoeff?
> 
> Thanks
> Mala
> 
> 
> 
> 
> 
> 
> 
> 
> 
> Holy Name Medical Center is ranked among the top hospitals in the nation 
> for patient care, clinical performance and workplace excellence.
> Click here to learn more.
> 
>  Warning: The information contained in this message is privileged and 
> CONFIDENTIAL and is intended only for the use of the addressee above. If 
> you are not the intended recipient, you are hereby notified that any 
> disclosure, copying, distribution, or taking of any action in reliance on 
> the content of this message is strictly prohibited. If you have received 
> this communication in error, please notify the sender by replying to this 
> message, and then delete it from your system.
> 
> 
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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E-Mail: michael.g...@morphisto.de 
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[Histonet] Type of Nitrile Gloves in your lab

[Cooper, Brian via Histonet]

Hi,

Does anyone out there use a brand of nitrile glove that they really like?  The 
ones we use here are not so good.  When we pull them on, the part closet to the 
wrist often tears off, leaving behind a nice blue rubber band.  At least 
several gloves per box have tears in them before we even put them on.  One of 
our Quality Managers asked us to come up with alternatives.  Can you help me 
out?   You can email me offline if you don't want to name particular brands for 
everyone to see.  PLEASE HELP!!!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu


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[Histonet] Elastic stain

[Nirmala Srishan via Histonet]

Histonetters,

Is there someone who can recommend a Elastic stain other than Verhoeff?

Thanks
Mala









Holy Name Medical Center is ranked among the top hospitals in the nation 
for patient care, clinical performance and workplace excellence.
Click here to learn more.

 Warning: The information contained in this message is privileged and 
CONFIDENTIAL and is intended only for the use of the addressee above. If 
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message, and then delete it from your system.




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[Histonet] Histology Supervisor Job

[Mighnon Lashus via Histonet]

We are currently looking for a Histology Supervisor at our derm lab in Atlanta, 
see information below.

Histology Supervisor
Location: Atlanta, GA
JOB DESCRIPTION
Schedule: Mon- Fri 11:00am-7:00pm
JOB SUMMARY:
Histology Supervisor directly supervises and coordinates the activities of the 
histology team including performing routine duties of histology including 
embedding, microtome, specials stains and related tasks.
ESSENTIAL FUNCTIONS:

  *   Support PathGroup's mission, vision, goals and management decisions.
  *   Provide leadership and supervise/manage assigned department, make 
decisions, solve problems, develop and document procedures, and conduct and 
attend meetings.
  *   Manage the employee hiring process including interviewing prospective 
employees, developing performance expectations, identifying essential functions 
and knowledge, skills and abilities required for applicable positions.
  *   Responsible for new employee orientation processes, employee training and 
continuing education.
  *   Manage employee performance by coaching, counseling, motivating, and 
evaluating employees on a continual basis. Implement disciplinary action as 
needed and in consultation with Human Resources.
  *   Ensure effective employee relations by sustaining an ethical, 
non-discriminatory and safe work environment and establishing effective 
communication lines and methods. Identify and solve employee problems, manage 
conflict, and respond to grievances as needed.
  *   Monitor and evaluate lab operations, workflow, and employees on an 
on-going basis and direct re-assignment of job duties or changes in processes/ 
workflow as needed.
  *   Work closely with histology management team to optimize workflow, 
quality, efficiency, and productivity.
  *   Properly embed all types of tissue and keep embedding area clean.
  *   Properly section all types of paraffin embedded surgical specimens and 
keep microtome and workstation clean.
  *   Perform all special stains and prepare all required solutions.
  *   Assure that paraffin blocks and slides are filed properly.
  *   Maintain quality control records on all equipment. Clean, change 
solutions and properly set all tissue processors and have basic maintenance 
knowledge.
  *   Assure proper disposal and handling of hazardous waste to include xylene 
recycling (i.e., xylene, reagent alcohol, formaldehyde).
  *   Ensure routine maintenance of equipment and adequate stock of supplies.
  *   Maintain required departmental records and reports.
  *   Assist in the development and implementation of departmental policies and 
procedures and maintain department procedure manuals.
  *   Exercise all laboratory safety precautions and adhere to lab procedures 
as stated in procedure manuals.
  *   Perform all job responsibilities in alignment with the industry's best 
security practices and regulatory guidelines to protect the confidentiality, 
integrity, and availability of protected health information and other sensitive 
company data.
  *   Must be familiar with and abide by the Corporate Compliance Program and 
all corporate policies, including the Privacy and Security policies.

JOB REQUIREMENTS
EDUCATION & LICENSURE:

  *   High school diploma or GED required.
  *   Associates Degree or other qualified technical college two year 
certificate preferred.
  *   ASCP certification in Histology preferred.
  *   Maintains appropriate continuing education for certification.

REQUIREMENTS:

  *   Over one year supervisory experience preferred or completion of PathGroup 
management training program.
  *   Over five years' experience within the field of Histology required.
  *   Computer proficiency in MS Word, Excel, and Outlook required.
Atlanta Dermatopathology is an equal opportunity employer.

Please submit your resume to i...@atldermpath.com, 
or by mail to:

Atlanta Dermatopathology
Attn: R. Clay, Office Manager
1901 Phoenix Boulevard, Suite 210
Atlanta, GA 30349


Mighnon Lashus, HT (ASCP)
Laboratory Manager

PathGroup
4071 S. Access Rd, Suite 107
Chattanooga, TN 37406
423-493-0207
423-493-0208 fax
mlas...@pathgroup.com

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Security Officer at PathGroup immediately at 615-562-9255. Thank you
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