Re: [Histonet] mounting medium

[Morken, Timothy via Histonet]

We use Permaslip acrylic for manual coverslipping. Sakura media for our 
automated coverslipper. We have also used Richard-Allan CytoSeal with great 
resuls. We stopped using one called Quick-mount (comes in  tube) because they 
had a batch that was bad and the media turned cloudy and the slips dropped off 
after about 6 months. That has been a pain

Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-Original Message-
From: Reilly, Laurie via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, November 13, 2017 3:35 PM
To: Allan Wang; warda hassan; Histonet@lists.utsouthwestern.edu
Cc: Hautaniemi, Walter; Reilly, Sue; Reeks, Karen
Subject: Re: [Histonet] mounting medium

We also would be interested in other's thoughts on mounting media. We are 
having coverslips coming unstuck from some of our teaching slides after 3 or 4 
years. It is very frustrating when cover slipping manually and wondering how 
long it will last.

Thanks and regards, Laurie.

Mr. Laurie REILLY
Histopathology
Veterinary and Biomedical Sciences
James Cook University
Townsville  Qld.  4811
Australia.

Phone 07 4781 4468
Mobile 0448 957747


-Original Message-
From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, 14 November 2017 8:47 AM
To: warda hassan 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mounting medium

Hi,

This information would be very useful for me and probably others as well, since 
I've just arbitrarily chosen one.
Can you summarize the responses you received for us?

Allan

On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Hello to all
>
> Can anyone suggest which mouting medium is best that will help 
> preservation of staining properties without creating fading of stains 
> and bubbles on long run.
> Thanks alot
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] mounting medium

[Reilly, Laurie via Histonet]

We also would be interested in other's thoughts on mounting media. We are 
having coverslips coming unstuck from some of our teaching slides after 3 or 4 
years. It is very frustrating when cover slipping manually and wondering how 
long it will last.

Thanks and regards, Laurie.

Mr. Laurie REILLY
Histopathology
Veterinary and Biomedical Sciences
James Cook University
Townsville  Qld.  4811
Australia.

Phone 07 4781 4468
Mobile 0448 957747


-Original Message-
From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, 14 November 2017 8:47 AM
To: warda hassan 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mounting medium

Hi,

This information would be very useful for me and probably others as well, since 
I've just arbitrarily chosen one.
Can you summarize the responses you received for us?

Allan

On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Hello to all
>
> Can anyone suggest which mouting medium is best that will help 
> preservation of staining properties without creating fading of stains 
> and bubbles on long run.
> Thanks alot
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] mounting medium

[Allan Wang via Histonet]

Hi,

This information would be very useful for me and probably others as well,
since I've just arbitrarily chosen one.
Can you summarize the responses you received for us?

Allan

On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello to all
>
> Can anyone suggest which mouting medium is best that will help preservation
> of staining properties without creating fading of stains and bubbles on
> long run.
> Thanks alot
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] validation

[Fulton Regan via Histonet]

Hello Nancy, 
 
Given your interest in best practices for validations,  I’d like to offer some 
recent literature references that may be of some use.
 
Your use of 20 positives and 20 negatives exceeds the minimum standard, from 
CAP Guidance. For purely diagnostic markers. (10+ and 10- are suggested in such 
cases), while 20+ and 20- are suggested as a minimum for predictive markers, 
such as ER, PR, etc… Your practice exceeds the minimum, but is certainly an 
excellent approach for better control of your assays!  
 
Patrick, L. F., Linda, A. B., Lisa, A. F., Alsabeh, R., Regan, S. F., Jeffrey, 
D. G., … Swanson, P. E. (2014). Principles of analytic validation of 
immunohistochemical assays: Guideline from the College of American Pathologists 
Pathology and Laboratory Quality Center. Archives of Pathology and Laboratory 
Medicine, 138(11), 1432–1443. https://doi.org/10.5858/arpa.2013-0610-CP
 
With respect to negative controls, there is another good reference, below:
 
Torlakovic, E. E., Francis, G., Garratt, J., Gilks, B., Hyjek, E., Ibrahim, M., 
… Vyberg, M. (2014). Standardization of Negative Controls in Diagnostic 
Immunohistochemistry. Applied Immunohistochemistry & Molecular Morphology, 
22(4), 241–252. https://doi.org/10.1097/PAI.0069
 
Internal negative control elements can definitely be part of the validation 
plan and can be used in specificity calculations.  Of course, it is important 
to confirm that these internal negatives are in fact negative!  However, it is 
also best practice to design validations as “fit for purpose”. That is,  does 
the assay reliably distinguish among the differential diagnostic  
considerations?  So, for specificity, a collection of “pertinent negatives” 
should also be included in the validation.  As an example: in the case of 
Beta-catenin for the diagnosis of fibromatosis, one should include some cases 
that resemble fibromatosis.  These might include, GIST, SCHWANNOMA, LEIOMYOMA, 
NEUROFIBROMA, and others, as your question clearly anticipated. 
 
I hope this of some use to you. 
 
Feel free to contact me if I can be of any further help. 
 
Regan



Regan Fulton, MD/PhD
 
CEO
Array Science, LLC
Tissue Microarray Technologies
475 Gate 5 Road, #102
Sausalito, CA 94965

ful...@arrayscience.com

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] The Use of Plants in Histology Laboratories

[Mickley, Beth via Histonet]

I found this great article about plants used in laboratories:

Plants That Can Clean Up Your Indoor Air

Plants clean indoor air in two ways—by absorbing contaminants through pores on 
the leaves, and by metabolizing contaminants through organisms living in the 
soil. In fact, plants are so effective that some stores, like Lowe’s and Home 
Depot, are starting to label the most effective ones with tags. 

Though it seems most plants will benefit indoor air, the following are those 
that have been shown in scientific studies and shown to work. These plants can 
also help maintain humidity levels and remove mold spores and bacteria from the 
air. 
1.Spider Plant: formaldehyde, xylene and toluene.
2.Golden Pothos: benzene, formaldehyde, trichloroethylene, xylene and toluene.
3.Snake Plant (Mother-in-Law’s Tongue): benzene, formaldehyde, 
trichloroethylene, xylene and toluene.
4.Bamboo Palm or Reed Palm: formaldehyde, xylene, and toluene.
5.Chinese Evergreen: benzene, formaldehyde.
6.Peace Lily: benzene, formaldehyde, trichloroethylene, xylene, toluene, and 
ammonia.
7.English Ivy: mold and mildew, formaldehyde, benzene, xylene, and toluene.
8.Gerbera Daisies: benzene, formaldehyde, trichloroethylene.
9.Red-Edged Dracaena (Dracaena Marginata): benzene, formaldehyde, 
trichloroethylene, xylene, and toluene. 
10.Warneck Dracaena: benzene, trichloroethylene, xylene, and toluene.
11.Weeping Fig: formaldehyde, xylene, and toluene.
12.Chrysanthemum: formaldehyde, benzene, trichloroethylene, xylene, toluene, 
and ammonia.
13.Boston fern: formaldehyde, xylene and toluene.
14.Philodendron: formaldehyde.


Beth Geer, HT
Mohs Surgery
University Dermatology Associates
Rochester, NY



From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Monday, November 13, 2017 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 168, Issue 10

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA=k8wthWMVkqXuqUqqzZHV30GZnwgu7td9mpPmY3_7vBk=3j2a9oNdNzfMT-HS8S1P-6g49hennezEvpfXg6SM2B8=TcVH5qahDDnUM0zmI0Mzv1GfaZLh-hKDifP2KHwf1J8=
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. The Use of Plants in Histology Laboratories (Bharti Parihar)
   2. EDTA decalcification tissue issues (Dorothy Hu)
   3. Obituary - pathologist Bernard Leon Klionsky (Bob Richmond)


--

Message: 1
Date: Sun, 12 Nov 2017 19:41:03 -0800
From: Bharti Parihar 
To: Histonet Archive 
Subject: [Histonet] The Use of Plants in Histology Laboratories
Message-ID:

Content-Type: text/plain; charset="UTF-8"

Hello all! Are there any labs out there using plants in their processing
rooms to reduce formaldehyde and xylene fumes? If so, please share how you
went about this and if there have been issues with contamination?

--
Bharti Parihar, HT (ASCP)CM


--

Message: 2
Date: Mon, 13 Nov 2017 09:52:21 -0500
From: Dorothy Hu 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] EDTA decalcification tissue issues
Message-ID:

[Histonet] Obituary - pathologist Bernard Leon Klionsky

[Bob Richmond via Histonet]

With his permission I post pathologist Leon Metlay's Facebook obituary of
his wife's father:

My father-in-law, Dr. Bernard Leon Klionsky, died [November 11th, 2017] at
the age of 92. He was one of the unsung heroes of pathology. As a young
man, he invented the form of cryostat that we all use to this day, with the
microtome down inside the freezer. He was one of the greatest teachers I've
ever known. He was primarily known as a cytopathologist, but was also an
early member of the Pediatric Pathology Club.

As a child, during the Depression, he sold ice cream from a box on his
bicycle. He realized that the ice cream stayed cold with the opening on the
top. He applied that to cryostats. The paper was (I think) in AJCP. Some
time in the late '50s.

I asked him: What was his relationship to International / Damon / IEC (I
don't remember the exact name), that as far as I know brought out the first
practical refrigerated microtomes? I think they came along in 1960, and
were in common use by the time I got into pathology in 1964, though the old
"wet knife" method continued in common use into the 1970s.

He replied: He had no relationship with any manufacturer as far as I know.
As I heard it, he tried to put the idea in the public domain, so that
cryostats would be less expensive. I don't know what happened. The
University of Kansas got a free cryostat while he was there. When he moved
on to Pitt, the manufacturer took back the cryostat.

Does any of our old-timers remember any of this?

Bob Richmond

Samurai Pathologist

Maryville TN
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] EDTA decalcification tissue issues

[Dorothy Hu via Histonet]

We didn't compare EDTA with formic acid. But heard that formic acid also
can do many IHC and ISH, if not all the antibodies and all probes. I always
have problem of bone falling off, no matter what kind of slides. Thinking
both PFA and EDTA affect on this issue. So trying frozen tape unfixed,
undecalcified bone now. If anyone has research paper regarding this, please
share.
Thanks.

Dorothy Hu
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet