Re: [Histonet] Histonet Digest, Vol 171, Issue 15

[Diane Mahovlic via Histonet]

I use methyl acetate to de-plasticize my PMMA slides.. . 30 minutes, air
dry for a minute or two then re-hydrate in 100% and 95% alcohol, rinse in
water.  I make my own PMMA ,rather than using Technovit, maybe that has
something to do with it.  Has worked for me for 25+ years!

Diane

Diane Mahovlic, HT(ASCP)
Cleveland Clinic,
Cleveland, Ohio

On Fri, Feb 16, 2018 at 1:00 PM, 
wrote:

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>
>
> Today's Topics:
>
>1. Re: Deplastifying MMA (Kusters, Ronald)
>2. Re: [EXTERNAL] Deplastifying MMA (Dorothy Hu)
>3. P16 Antibody (Jason McGough)
>
>
> --
>
> Message: 1
> Date: Fri, 16 Feb 2018 09:50:15 +
> From: "Kusters, Ronald" 
> To: Reuel Cornelia ,
> "histonet@lists.utsouthwestern.edu"
> 
> Subject: Re: [Histonet] Deplastifying MMA
> Message-ID: <8e78115855214a058d4e6ccc137a2...@ex-pfm-103-w.pfm-ag.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Dear Reuel,
> Maybe the Kulkzer Technovit manufacturers website provides you more
> information or you could contact their specialists directly through this
> link:
> http://kulzer-technik.de/en_kt/kt/maerkte/histologie/
> startseite_histologie.aspx
>
> Yours,
> Ronald
>
> Mit freundlichen Gr??en/Kind Regards
>
>
>  Ronald Kusters
> International Sales Manager Feather Brand Histotechnology
>
> pfm medical ag
> Wankelstra?e 60
> 50996 K?ln, Germany
> T +49 (2236) 9641 660
> F +49 (2236) 9641 99 660
> M +49 (171) 9144343
>
>
>
> -Original Message-
> From: Reuel Cornelia [mailto:reuel.corne...@tsrh.org]
> Sent: maandag 12 februari 2018 22:07
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Deplastifying MMA
>
> Hello histonetters,
>
> I have a problem with my MMA plastic section on my slide. I could not
> remove the plastic MMA even if I leave the section slides in Xylene for
> several days with heat incubation at 60 degrees. This is the first time
> that happened to my section for several years of doing the same thing over.
> I was thinking that it was my Xylene lot number so I tried to change it but
> still does not remove the plastic, and then Itried different solvent
> Acetone, and Ethylene glycol Monoethyl ether but still it does not work.
> Can I ask for help if anyone knows what was going on and what would be the
> best way to remove this plastic from my section?
>
> Just to give you my embedding solution was MMA -94% , dibutly phthalate-
> 5% and perkadox 16- 0.5%. This have been my solution for years and I do not
> have any problem with the removal of plastic section until now. I was
> thinking that my MMA (M55909) different lot number from Sigma Aldrich may
> have cause this because even I tried to use the MMA to dissolve my plastic
> it does not work.
>
>
> Thank you for and any opinions or protocols are greatly appreciated.
>
>
> Reuel Cornelia
>
> TSRH
>
> 214-559-7766
>
> --
> Texas Scottish Rite Hospital for Children is one of the nation's leading
> pediatric centers for the treatment of orthopedic conditions, certain
> related neurological disorders and learning disorders, such as dyslexia.
> This email transmission and/or its attachments may contain confidential
> health information, intended only for the use of the individual or entity
> named above.
>
>
>
>
> --
>
> Message: 2
> Date: Fri, 16 Feb 2018 08:34:42 -0500
> From: Dorothy Hu 
> To: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] [EXTERNAL] Deplastifying MMA
> Message-ID:
>  mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Hi Lori,
>
> Would you please tell me which company and catalog # of  tetrahydrofuran
> you used before?
> For how many minutes you did for deplastifying in tetrahydrofuran?
> Thanks in advance.
>
> Dorothy
>
> Message: 3
> Date: Wed, 14 Feb 2018 20:05:16 +
> From: "Garcia, Lori, M.Sc." 
> To: "reuel.corne...@tsrh.org" 
> Cc: "histonet@lists.utsouthwestern.edu"
> 
> Subject:
> Message-ID:
>  namprd05.prod.outlook.com>
>

Re: [Histonet] Histonet Digest, Vol 171, Issue 15

[Madeleine Huey via Histonet]

 Subject: [Histonet] P16 Antibody
Jason, I also tried many different vendors & clones many years ago (human
FFPE tissues), and the winner is from Roche/Ventana (CINtec, Cat. 9517 =
11.5ml).  You can get 5ml dispenser as well.


*Madeleine Huey**, HTL & QIHC (ASCP)*

Supervisor - Pathology (Histology & IPOX)

El Camino Hospital, and Los Gatos Hospital

2500 Grant Rd (Rm #GC-413), Mountain View, CA 94040-4378

Phone:  650-940-7038

Fax:  650-966-9292

Email:  *madelein...@elcaminohospital.org
*


On Fri, Feb 16, 2018 at 10:00 AM,  wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>1. Re: Deplastifying MMA (Kusters, Ronald)
>2. Re: [EXTERNAL] Deplastifying MMA (Dorothy Hu)
>3. P16 Antibody (Jason McGough)
>
>
> --
>
> Message: 1
> Date: Fri, 16 Feb 2018 09:50:15 +
> From: "Kusters, Ronald" 
> To: Reuel Cornelia ,
> "histonet@lists.utsouthwestern.edu"
> 
> Subject: Re: [Histonet] Deplastifying MMA
> Message-ID: <8e78115855214a058d4e6ccc137a2...@ex-pfm-103-w.pfm-ag.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Dear Reuel,
> Maybe the Kulkzer Technovit manufacturers website provides you more
> information or you could contact their specialists directly through this
> link:
> http://kulzer-technik.de/en_kt/kt/maerkte/histologie/
> startseite_histologie.aspx
>
> Yours,
> Ronald
>
> Mit freundlichen Gr??en/Kind Regards
>
>
>  Ronald Kusters
> International Sales Manager Feather Brand Histotechnology
>
> pfm medical ag
> Wankelstra?e 60
> 50996 K?ln, Germany
> T +49 (2236) 9641 660
> F +49 (2236) 9641 99 660
> M +49 (171) 9144343
>
>
>
> -Original Message-
> From: Reuel Cornelia [mailto:reuel.corne...@tsrh.org]
> Sent: maandag 12 februari 2018 22:07
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Deplastifying MMA
>
> Hello histonetters,
>
> I have a problem with my MMA plastic section on my slide. I could not
> remove the plastic MMA even if I leave the section slides in Xylene for
> several days with heat incubation at 60 degrees. This is the first time
> that happened to my section for several years of doing the same thing over.
> I was thinking that it was my Xylene lot number so I tried to change it but
> still does not remove the plastic, and then Itried different solvent
> Acetone, and Ethylene glycol Monoethyl ether but still it does not work.
> Can I ask for help if anyone knows what was going on and what would be the
> best way to remove this plastic from my section?
>
> Just to give you my embedding solution was MMA -94% , dibutly phthalate-
> 5% and perkadox 16- 0.5%. This have been my solution for years and I do not
> have any problem with the removal of plastic section until now. I was
> thinking that my MMA (M55909) different lot number from Sigma Aldrich may
> have cause this because even I tried to use the MMA to dissolve my plastic
> it does not work.
>
>
> Thank you for and any opinions or protocols are greatly appreciated.
>
>
> Reuel Cornelia
>
> TSRH
>
> 214-559-7766
>
> --
> Texas Scottish Rite Hospital for Children is one of the nation's leading
> pediatric centers for the treatment of orthopedic conditions, certain
> related neurological disorders and learning disorders, such as dyslexia.
> This email transmission and/or its attachments may contain confidential
> health information, intended only for the use of the individual or entity
> named above.
>
>
>
>
> --
>
> Message: 2
> Date: Fri, 16 Feb 2018 08:34:42 -0500
> From: Dorothy Hu 
> To: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] [EXTERNAL] Deplastifying MMA
> Message-ID:
>  mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Hi Lori,
>
> Would you please tell me which company and catalog # of  tetrahydrofuran
> you used before?
> For how many minutes you did for deplastifying in tetrahydrofuran?
> Thanks in advance.
>
> Dorothy
>
> Message: 3
> Date: Wed, 14 Feb 2018 20:05:16 +
> From: "Garcia, Lori, M.Sc." 
> To: "reuel.corne...@tsrh.org" 
> Cc: 

[Histonet] used cryostats

[Cynthia McGrady via Histonet]

Hi.
Our lab is in the market for a used or refurbished cryostat. Any tips or lead 
would be greatly appreciated. Thank.. Cindy McGrady, Hurley Medical Center, 
Flint, Michigan.
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[Histonet] P16 Antibody

[Jason McGough via Histonet]

We are looking to bring in P16 to do in house but are having a hard time 
getting the stain to be clean. We are having issues with positive staining on 
tissue that should be negative. We are working with a concentrated antibody 
that we have diluted out to 1:6000 (which is 6 times the amount suggested from 
vendors 1:200-1:1000). We might need to change clones or vendors and were 
wondering if you could tell us what kind of p16 (clone) you use for staining 
and possible what vendor you get it from? Any information or suggestions you 
have would greatly be appreciated. Thank you.


Jason McGough, HT(ASCP)

Operations Manager
Clinical Laboratory of the Black Hills
605-343-2267
jmcgo...@clinlab.com
www.clinlab.com

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Re: [Histonet] [EXTERNAL] Deplastifying MMA

[Dorothy Hu via Histonet]

Hi Lori,

Would you please tell me which company and catalog # of  tetrahydrofuran
you used before?
For how many minutes you did for deplastifying in tetrahydrofuran?
Thanks in advance.

Dorothy

Message: 3
Date: Wed, 14 Feb 2018 20:05:16 +
From: "Garcia, Lori, M.Sc." 
To: "reuel.corne...@tsrh.org" 
Cc: "histonet@lists.utsouthwestern.edu"

Subject:
Message-ID:


Content-Type: text/plain; charset="us-ascii"

Hi Reuel,

One thing you could try is THF (tetrahydrofuran). We have used it in the
past to rapidly dissolve the MMA on slides.

Good luck,
Lori
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Re: [Histonet] Deplastifying MMA

[Kusters, Ronald via Histonet]

Dear Reuel,
Maybe the Kulkzer Technovit manufacturers website provides you more information 
or you could contact their specialists directly through this link:
http://kulzer-technik.de/en_kt/kt/maerkte/histologie/startseite_histologie.aspx 

Yours,
Ronald

Mit freundlichen Grüßen/Kind Regards


 Ronald Kusters
International Sales Manager Feather Brand Histotechnology 

pfm medical ag 
Wankelstraße 60
50996 Köln, Germany 
T +49 (2236) 9641 660 
F +49 (2236) 9641 99 660
M +49 (171) 9144343



-Original Message-
From: Reuel Cornelia [mailto:reuel.corne...@tsrh.org] 
Sent: maandag 12 februari 2018 22:07
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Deplastifying MMA

Hello histonetters,

I have a problem with my MMA plastic section on my slide. I could not remove 
the plastic MMA even if I leave the section slides in Xylene for several days 
with heat incubation at 60 degrees. This is the first time that happened to my 
section for several years of doing the same thing over. I was thinking that it 
was my Xylene lot number so I tried to change it but still does not remove the 
plastic, and then Itried different solvent Acetone, and Ethylene glycol 
Monoethyl ether but still it does not work. Can I ask for help if anyone knows 
what was going on and what would be the best way to remove this plastic from my 
section?

Just to give you my embedding solution was MMA -94% , dibutly phthalate- 5% and 
perkadox 16- 0.5%. This have been my solution for years and I do not have any 
problem with the removal of plastic section until now. I was thinking that my 
MMA (M55909) different lot number from Sigma Aldrich may have cause this 
because even I tried to use the MMA to dissolve my plastic it does not work.


Thank you for and any opinions or protocols are greatly appreciated.


Reuel Cornelia

TSRH

214-559-7766

--
Texas Scottish Rite Hospital for Children is one of the nation's leading 
pediatric centers for the treatment of orthopedic conditions, certain related 
neurological disorders and learning disorders, such as dyslexia. 
This email transmission and/or its attachments may contain confidential health 
information, intended only for the use of the individual or entity named above.


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