Re: [Histonet] benchtop fume hoods
We are using a portable fume extractor from Sentry Air Systems for removing xylene fumes from our coverslipper. It's called the SS-200-WS, it has adjustable 'wings' and you can also buy one with shielding. We like it so much we bought another 5. The carbon filters make all the difference, our first filter lasted almost 1500 hours before needing replacement. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Thu, May 10, 2018 at 8:25 AM, Histology via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > Can anyone share what type of benchtop fume hoods they are using in the > lab? This would be for manual coverslipping and grossing. > > Thanks in advance! > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 174, Issue 7- Benchtop fume hood. (Alan Wagg)
Hey Alan, I use a Airfiltronix Ductless Bench Top Hood and it meets all my needs. I gross about 7500-8000 GI biopsies per year and do any hand cover slipping under this hood (which is rare if I can help it). The unit is made of clear acrylic, is see thru and is framed out in light weight aluminum. It has a clear Velcroed thick mil roll up sash which is very easy to work with. The system has two blower units on top. Both units have 2 inch carbon filters that I purchase from Mercedes Medical and replace twice a year. I did add a grated plastic secondary containment box which I bought through Grainger to place the unit on so that I could keep my formalin cube under the hood and not worry about spillage. This also elevated the work surface off the counter by 8-10 inches so you are not leaning over the work and therefore saving your back. I had this unit tested by an outside agency along with the whole lab before we started operation and then again after we started operation. Our PEL numbers for Formalin, Alcohol and Xylene were barley negligible. I have used the badge system every year since the original testing with the same results. This to me is the key to selecting any hood, does it keep the PEL numbers within exposure limits. The purchase price is around $2800.00 + filters @ $180 ea. Hope this helps and good luck. Brent Adams – BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 From: histonet-requ...@lists.utsouthwestern.eduSent: Thursday, May 10, 2018 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 174, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Paraffin infiltration issue, will this impact IHC? (Greg Dobbin) 2. Histotech Position - Chandler AZ (Office Manager) 3. IHC and Histogel (Paula) 4. Re: IHC and Histogel (dusko trajkovic) 5. Re: IHC and Histogel (John Shelley) 6. Shandon Veristain Gemini Autostainer (Charles Riley) 7. benchtop fume hoods (Histology) 8. Re: Shandon Veristain Gemini Autostainer (Mike Toole) 9. Re: benchtop fume hoods (Allan Wang) -- Message: 1 Date: Wed, 9 May 2018 15:26:57 -0300 From: Greg Dobbin To: modz9...@gmail.com, histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin infiltration issue, will this impact IHC? Message-ID:
Re: [Histonet] benchtop fume hoods
Hi Allan, Not trying to advertise, but, we have been making a Downdraft Fume Extraction Workstation for 40+ years. Lots of folks in Histoland have had one at one time or another. Perfect for coverslipping and/or grossing. If you are interested I would be glad to send you some info. Jim Best Regards, James Mullen, Jr., P.E. Vice President Hacker Instruments & Industries, Inc. 1132 Kincaid Bridge Road PO Box 1176 Winnsboro, SC 29180 www.hackerinstruments.com (803) 712-6100 -Original Message- From: Allan Wang via HistonetCc: histonet Sent: Thu, May 10, 2018 1:01 pm Subject: Re: [Histonet] benchtop fume hoods I'd like to know as well. Thanks, Allan On Thu, May 10, 2018 at 11:25 AM, Histology via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > Can anyone share what type of benchtop fume hoods they are using in the > lab? This would be for manual coverslipping and grossing. > > Thanks in advance! > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] benchtop fume hoods
I use a Labconco. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Allan Wang via HistonetTo: Cc: "histonet@lists.utsouthwestern.edu" Sent: Thursday, May 10, 2018 12:02 PM Subject: Re: [Histonet] benchtop fume hoods I'd like to know as well. Thanks, Allan On Thu, May 10, 2018 at 11:25 AM, Histology via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > Can anyone share what type of benchtop fume hoods they are using in the > lab? This would be for manual coverslipping and grossing. > > Thanks in advance! > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] benchtop fume hoods
I'd like to know as well. Thanks, Allan On Thu, May 10, 2018 at 11:25 AM, Histology via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > Can anyone share what type of benchtop fume hoods they are using in the > lab? This would be for manual coverslipping and grossing. > > Thanks in advance! > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Shandon Veristain Gemini Autostainer
Charles, The issue is most likely the handle for the rack, The rack has a moveable handle. Make sure the handle is securely in the upright position. It has pins and holes that should line up and click into place. If it is even slightly at an angle or not securely snapped into an upright position it may bump into the side of the stain pots (and really cause a mess). Mike -Original Message- From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, May 10, 2018 9:51 AM To: Histo List Subject: [EXTERNAL] [Histonet] Shandon Veristain Gemini Autostainer Has anyone had any issues with this tissue stainer dropping or crushing racks? We use it to run our gyn and non gyn pap/thin prep slides. Recently it has been tipping over the slide racks in the middle of the procedure and breaking the slides -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] benchtop fume hoods
Hi all, Can anyone share what type of benchtop fume hoods they are using in the lab? This would be for manual coverslipping and grossing. Thanks in advance! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Shandon Veristain Gemini Autostainer
Has anyone had any issues with this tissue stainer dropping or crushing racks? We use it to run our gyn and non gyn pap/thin prep slides. Recently it has been tipping over the slide racks in the middle of the procedure and breaking the slides -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC and Histogel
We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko On Thursday, May 10, 2018 7:13 AM, Paula via Histonetwrote: Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC and Histogel
I agree with Dusko, I have used histogel the same way and have also used it for FISH and ISH without any issues. Kind Regards! John J Shelley Histology Core Manager Sanford Burnham Prebys Medical Discovery Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Lab: (407) 745-2119 Fax: (407) 745-2001 email: jshel...@sbpdiscovery.org Please note email change -Original Message- From: dusko trajkovic via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, May 10, 2018 10:20 AM To: Paula; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC and Histogel We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko On Thursday, May 10, 2018 7:13 AM, Paula via Histonetwrote: Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet=02%7C01%7Cjshelley%40sbpdiscovery.org%7C217545f9b96c4e33e47a08d5b6812003%7C0b162723004547deb0699f1a7aa955a1%7C0%7C0%7C636615588083420177=LTCmir6KeMPW%2FGwPz5fdtjioshiu0OhqLYjhh6zJSPg%3D=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet=02%7C01%7Cjshelley%40sbpdiscovery.org%7C217545f9b96c4e33e47a08d5b6812003%7C0b162723004547deb0699f1a7aa955a1%7C0%7C0%7C636615588083420177=LTCmir6KeMPW%2FGwPz5fdtjioshiu0OhqLYjhh6zJSPg%3D=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC and Histogel
Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet