Re: [Histonet] Histonet Digest, Vol 174, Issue 9

[Bob Richmond via Histonet]

Jamie Watson, you need to clarify this question: >> Does anyone know what
percentage of labs use ethanol vs. denatured alcohol or isopropyl alcohol
on the tissue processor?<<

100% ethanol is perfectly OK, but it requires a lot of regulator compliance
because you can drink it (though you shouldn't).

The usual denatured alcohol for histology is customarily called "reagent
alcohol". It contains 90% ethanol, 5% methanol, and 5% isopropanol. The
BATF calls it "S3D modified". It's also possible to denature with methyl
isobutyl ketone (MIBK) but it smells horrible. Alcohol denatured with
acetone is available, but it'll extract your eosin - you need to know about
it because an occasional manager will insist on it.

All of these are equivalent from a practical viewpoint. Isopropyl alcohol
(isopropanol) is of course quite different, and I have no idea how many
labs use it.

Bob Richmond
Samurai Pathologist
Maryville TN

On Sat, May 12, 2018 at 1:00 PM, 
wrote:

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>1. Ethanol in tissue processing (Jamie Watson)
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> Date: Sat, 12 May 2018 16:06:01 +
> From: Jamie Watson 
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> Subject: [Histonet] Ethanol in tissue processing
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> Hi All,
>
> Does anyone know what percentage of labs use ethanol vs. denatured alcohol
> or isopropyl alcohol on the tissue processor?
>
> Jamie
>
>
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> End of Histonet Digest, Vol 174, Issue 9
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[Иванов Иван via Histonet]

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Re: [Histonet] Ethanol in tissue processing

[Michael Gudo (Morphisto GmbH) via Histonet]

Well, in Germany, and as far as I know in Europe, most labs use ethanol at 96 
and 99 percent as the last two steps in tissue processing. These ethanols are 
are denatured with MEK (= Butanon, Methylethylketon). Methanol or isopropyl is 
not allowed as denaturation agent in Europe, otherwise you have to pay the full 
alcohol tax.

In our lab we we use 96 % ethanol and as last step and then,  before going to 
xylene we use isopropanol which has a better dehydration effect than ethanol. 
99 % ethanol takes water from the air and quite fast becomes a 96 or 95 % 
ethanol and you will have some water left in the specimens when you to to 
xylene.
So our experience are that the best quality of dehydration can be reached with 
ethanol as high as possible and finally isopropanol before the xylene (2 
steps). 

With best regards
Michael


> Am 12.05.2018 um 22:48 schrieb Jay Lundgren via Histonet 
> :
> 
> Most places use "reagent alcohol" which, from most suppliers, is a mongrel
> blend of whatever is cheapest, and not even listed on the label. It's
> usually made up of a blend of ethanol, methanol, and isopropyl.  Good luck
> finding what the percentages are.  I've called one vendor before, and even
> they couldn't tell me.
> 
> 
> Virus-free.
> www.avg.com
> 
> <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>
> 
> On Sat, May 12, 2018 at 2:48 PM, Mac Donald, Jennifer via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> 
>> We use reagent alcohol on the processor and for staining.
>> 
>> Get Outlook for iOS
>> 
>> From: Jamie Watson via Histonet 
>> Sent: Saturday, May 12, 2018 9:06:01 AM
>> To: Histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] Ethanol in tissue processing
>> 
>> Hi All,
>> 
>> Does anyone know what percentage of labs use ethanol vs. denatured alcohol
>> or isopropyl alcohol on the tissue processor?
>> 
>> Jamie
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

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Re: [Histonet] Ethanol in tissue processing

[Jay Lundgren via Histonet]

Most places use "reagent alcohol" which, from most suppliers, is a mongrel
blend of whatever is cheapest, and not even listed on the label. It's
usually made up of a blend of ethanol, methanol, and isopropyl.  Good luck
finding what the percentages are.  I've called one vendor before, and even
they couldn't tell me.


Virus-free.
www.avg.com

<#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>

On Sat, May 12, 2018 at 2:48 PM, Mac Donald, Jennifer via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> We use reagent alcohol on the processor and for staining.
>
> Get Outlook for iOS
> 
> From: Jamie Watson via Histonet 
> Sent: Saturday, May 12, 2018 9:06:01 AM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Ethanol in tissue processing
>
> Hi All,
>
> Does anyone know what percentage of labs use ethanol vs. denatured alcohol
> or isopropyl alcohol on the tissue processor?
>
> Jamie
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Re: [Histonet] Ethanol in tissue processing

[Mac Donald, Jennifer via Histonet]

We use reagent alcohol on the processor and for staining.

Get Outlook for iOS

From: Jamie Watson via Histonet 
Sent: Saturday, May 12, 2018 9:06:01 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ethanol in tissue processing

Hi All,

Does anyone know what percentage of labs use ethanol vs. denatured alcohol or 
isopropyl alcohol on the tissue processor?

Jamie
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[Histonet] Ethanol in tissue processing

[Jamie Watson via Histonet]

Hi All,

Does anyone know what percentage of labs use ethanol vs. denatured alcohol or 
isopropyl alcohol on the tissue processor?

Jamie
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