[Histonet] job opening

[Blazek, Linda via Histonet]

We have an opening for a Histology Technician. This position is Monday - Friday 
day shift position.  We are a growing company that has just opened a brand new 
facility, with windows!  You must be Certified or eligible for Board of 
Certification (BOC) by the American Society of Clinical Pathologists (ASCP) and 
have a minimum of one year of experience as a Histology Technician with 
competency in the areas of processing, embedding, microtomy and with a basic 
knowledge of immunohistochemistry.  We only do a minimum number of special 
stains.  We work hard to encourage team values, open honest communication and a 
system of continuous quality improvement.
For consideration for this position, please send a cover letter and your resume 
to 
dsiemploym...@digestivespecialists.com
 and place your name on the subject line of the email, or contact me directly.


Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


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[Histonet] (no subject)

[Cindy Bird via Histonet]

Hello All,

I am curious to see what chemical everyone uses to decal bone marrow biopsies?

Cindy Bird
Anatomical Medical Laboratories, Inc.
1600 Scripture Street
Denton, TX  76201
940-384-6210
940-384-6000
Fax 940-565-9588

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[Histonet] p16 and sperm morphology

[Nancy Schmitt via Histonet]

Hello-


1.   We are currently running P16 on the Leica Bond Max - are you running 
or reporting this any differently now that they are 510k FDA approved?


2.   Sperm Morphology- are your cytotechnologists reading and reporting out 
sperm morphology or are your clinical lab techs?


Responses appreciated:)

Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA  52001



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[Histonet] Leica Autostainer XL for special stains?

[Angela Denn via Histonet]

Has anybody used Leica's Autostainer for specials? There's a whitepaper on
Leica's website describing such use but I'd really like to hear some real
world opinions on using it in such a manner. I need an inexpensive,
refurbished unit that can handle my heavy hitters: H, PAS, and trichrome.
I am open to suggestions.



*Angela Lamberth Denn* | Histology Technician III
*La Jolla Institute for Allergy and Immunology*
Histology Core, Room 2328
9420 Athena Circle La Jolla, CA 92037
http://www.lji.org
858.752.
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Re: [Histonet] New Bone Stain

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hello Jessica,
Hello Natalia,

we have discussed the question of bone staining methods in our lab and we would 
recommend the following stainings, which will work on resin embedded material:

(1) For an overview and differentiation of various types of bone the 
traditional MASSON-GOLDNER or AZAN staining is quite fine, and should be the 
first try because these stains are more or less easy and fast and the staining 
solutions are not too expensive.

(2) MOVAT-Pentachrome in the original and in the modification after VERHOEFF 
also gives quite good differentiations of resin embedded bones and shows many 
details even in different types of bone. We use these staining here in many 
research projects we do for pharma companies that develop bone replacement 
materials.

(3) van KOSSA is also a very good silver impregnation to show mineralized bone, 
AND there is a possible combination with the MOVAT Pentachrom-Staining.

(4) Alizarinred can also be used to show different gradients of mineralisation; 
the staining kit contains solutions with several pH-values.

(5) Picro-Siriusred and other combinations of picric acid and other stains 
(picro fuchsin, picro polychrom, picro indigocarmin, and 
orcein-picroindicocramin can show good differentiations on tissues and 
cartilage and bone structures.
Remark: Sometimes its a bit difficult to establish picric acid based stainings 
on resin embedded tissues, if the resin has not been completely removed (which 
is possible with technovit 9100 (and some other MMA’s), but not with technovit 
7200).

(6) LEVAI-LACZKO's stain provides a good differentiation between mineralized 
bone matrix, Osteoid and synthetic bone substitutes.

(7) A special „Bone Stain“, published by Villanueva in 2009 
(https://www.tandfonline.com/doi/abs/10.3109/10520297409116928 
) works best on 
fresh material. We have not yet tested it completely on resin embedded tissues, 
but some of our customers used it successfully.
For this stain, a „staining powder" has to be synthesized from various single 
stains (basic fuchsin, orange G, fastgreen and azure II) and finally mixed into 
a ready to use staining solution; we produce the stain powder and ready to use 
solution here in our lab.

(8) Last but not least HEROVICI’s stain might help beacuse it differentiates 
between new and old collagen structures. Though it was originally developed for 
diagnostics in wound repair, some of our customers use it to differentiate 
between mineralized bone, new bone (Osteoid) and synthetic bone substitutes.

For all staining procedures on resin embedded material its necessary to edge 
the surface with citric acid or hydrogen peroxide, depending on the kind of 
resin you are using. If it is technovit 7200 citric acid should work fine, for 
technovit 9100 you can also try to remove the resin with methoxyethylacetate 
and acetone. Please note, that there can be a significant difference in the 
quality of the stain between Technovit 9100 and Technovit 7200, depending on 
what you want to see. In general, the stain on Technovit 9100 sections will be 
more powerful and brilliant than that on Technovit 7200 sections. 

As mentioned in my previous mail, we have a local dealer in Oklahoma, and if 
you or somebody else is interested in our ready-to-use stainings kits, just let 
me know, I will then make the direct contact.

Kind regards
Michael



> Am 10.07.2018 um 22:15 schrieb Jessica Riggleman via Histonet 
> :
> 
> Hey Jennifer,
> Thanks for your help. I have two questions:
> 
> 1. What is the point of decalcifying if they are in plastic?
> 2. Did you see the counterstain? Right now only the bone is staining. How 
> long did you leave these in the counterstain for?
> Thanks!
> Jessica
> 
> From: Mac Donald, Jennifer [mailto:jmacdon...@mtsac.edu]
> Sent: Tuesday, July 10, 2018 4:07 PM
> To: Jessica Riggleman ; 
> histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] New Bone Stain
> 
> I used to do it on plastic sections of in-de-calcified bone biopsies. It’s a 
> silver reaction.
> 
> Get Outlook for iOS
> 
> 
> 
> _
> 
> Jessica Riggleman | Research Associate
> 
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
> 
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