Re: [Histonet] Sakura glas2

[Mac Donald, Jennifer via Histonet]

We use Permount and an aliphatic hydrocarbon clearing reagent.
Jennifer

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From: Bitting, Angela K. via Histonet 
Sent: Wednesday, July 18, 2018 8:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sakura glas2


What mounting medium are folks using with their Sakura glas2 coverslip 
instruments? We just purchased one.
Our lab is xylene-free so I want to avoid bringing a xylene-based product in.


Sent from Angie's iPhone

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[Cristi Rigazio via Histonet]

Have you checked the processors?  It is easy to “mix up” the reagents.  
Has tissue collection changed? Are the specimens left out longer or placed on 
paper? 
 Do you use sponges?  Any chance the fixation time has been decreased?

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> On Jul 18, 2018, at 3:46 PM, Mac Donald, Jennifer via Histonet 
>  wrote:
> 
> Is anyone using freezing spray?  We had this problem and all cases were 
> traced back to the same microtomist. She used the freezing spray excessively 
> and was causing “freezer burn” to the GI biopsies.
> Jennifer
> 
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> 
> 
> From: Charles Riley via Histonet 
> Sent: Wednesday, July 18, 2018 11:12 AM
> To: Histo List
> Subject: [Histonet] Hazy/cooked cells in H&E
> 
> Our pathologists have said that we have had a few cases the past few days
> that are unreadable. They say the cells appear "cooked".
> 
> Nothing in our process has changed, we have been using the same lot of
> reagents since the middle of June, and no errors were reported in our
> processing runs.
> 
> Do anyone have any ideas to what could be causing this issue?
> 
> 
> --
> 
> Charles Riley BS HT, HTL(ASCP)CM
> 
> Histopathology Coordinator/ Mohs
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[Mac Donald, Jennifer via Histonet]

Is anyone using freezing spray?  We had this problem and all cases were traced 
back to the same microtomist. She used the freezing spray excessively and was 
causing “freezer burn” to the GI biopsies.
Jennifer

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From: Charles Riley via Histonet 
Sent: Wednesday, July 18, 2018 11:12 AM
To: Histo List
Subject: [Histonet] Hazy/cooked cells in H&E

Our pathologists have said that we have had a few cases the past few days
that are unreadable. They say the cells appear "cooked".

Nothing in our process has changed, we have been using the same lot of
reagents since the middle of June, and no errors were reported in our
processing runs.

Do anyone have any ideas to what could be causing this issue?


--

Charles Riley BS HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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[Charles Riley via Histonet]

Our pathologists have said that we have had a few cases the past few days
that are unreadable. They say the cells appear "cooked".

Nothing in our process has changed, we have been using the same lot of
reagents since the middle of June, and no errors were reported in our
processing runs.

Do anyone have any ideas to what could be causing this issue?


-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] New Microtome

[Terri Braud via Histonet]

We are using a Leica 2235 and love it.  Manual or Auto mode, the control panel 
is on a moveable pad, so can be used anywhere.  It has a foot pedal if you like 
that too!  The techs took to it like a duck to water and it cuts like butter!

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal



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Re: [Histonet] Microtomes

[Laurie Colbert via Histonet]

We have three Thermo 355S's.  They are nice because you can use them in the 
automated mode or manual mode.  All of our HT's use the manual mode, so now we 
just buy the manual version.

Laurie Redmond HT (ASCP)
Path MD


-Original Message-
From: Piche, Jessica via Histonet 
To: histonet 
Sent: Wed, Jul 18, 2018 7:29 am
Subject: [Histonet] Microtomes

Good Morning Everyone,

We are in the market for new microtomes and was wondering what everyone is 
using out there? We are looking for semi-automated/automated microtomes. We are 
demoing the Leica Autocut and the Thermo 355S. I really like the Leica 
microtome but am not crazy about the control panel. We cut with our water baths 
to our left and the control panel is in the way. What are other techs doing? 
I'd appreciate your thoughts.

Thank you in advance.

Jessica Piche, HT(ASCP)
Waterbury Hospital



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[Histonet] Microtomes

[Piche, Jessica via Histonet]

Good Morning Everyone,

We are in the market for new microtomes and was wondering what everyone is 
using out there? We are looking for semi-automated/automated microtomes. We are 
demoing the Leica Autocut and the Thermo 355S. I really like the Leica 
microtome but am not crazy about the control panel. We cut with our water baths 
to our left and the control panel is in the way. What are other techs doing? 
I'd appreciate your thoughts.

Thank you in advance.

Jessica Piche, HT(ASCP)
Waterbury Hospital



CONFIDENTIALITY NOTICE: This email and any attachments contain confidential 
information that is legally privileged. This information is intended only for 
the use of the individual or entity named above. The authorized recipient of 
this information is prohibited from disclosing this information to any other 
party unless required to do so by law or regulation. If you are not the 
intended recipient, you are hereby notified that any disclosure, copying, 
distribution or action taken in reliance on the contents of these documents is 
strictly prohibited. If you have received this information in error, please 
notify the sender immediately and delete these documents. Copyright (c) 
Waterbury Hospital
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