Re: [Histonet] Unstained slides - how long are they good for?

[Tony Henwood (SCHN) via Histonet]

Will definitely depend on the antibody you are using. Some references:

Jacobs, T. W., Prioleau, J. E., Stillman, I. E., & Schnitt, S. J. (1996). Loss 
of tumor marker-immunostaining intensity on stored paraffin slides of breast 
cancer. JNCI: Journal of the National Cancer Institute, 88(15), 1054-1059.

Manne, U., MYERS, R. B., SRIVASTAVA, S., & GRIZZLE, W. E. (1997). Re: loss of 
tumor marker-immunostaining intensity on stored paraffin slides of breast 
cancer. Journal of the National Cancer Institute, 89(8), 585-586.

Bertheau, P., Cazals-Hatem, D., Meignin, V., de Roquancourt, A., Vérola, O., 
Lesourd, A., ... & Janin, A. (1998). Variability of immunohistochemical 
reactivity on stored paraffin slides. Journal of clinical pathology, 51(5), 
370-374.

Olapade-Olaopa, E. O., Mackay, E. H., & Habib, F. K. (1998). Variability of 
immunohistochemical reactivity on stored paraffin slides. Journal of clinical 
pathology, 51(12), 943.

Wester, K., Wahlund, E., Sundström, C., Ranefall, P., Bengtsson, E., Russell, 
P. J., ... & Busch, C. (2000). Paraffin section storage and 
immunohistochemistry: effects of time, temperature, fixation, and retrieval 
protocol with emphasis on p53 protein and MIB1 antigen. Applied 
Immunohistochemistry & Molecular Morphology, 8(1), 61-70.

van den Broek, L. J., & van de Vijver, M. J. (2000). Assessment of problems in 
diagnostic and research immunohistochemistry associated with epitope 
instability in stored paraffin sections. Applied Immunohistochemistry & 
Molecular Morphology, 8(4), 316-321. 

Olapade-Olaopa, E. O., Ogunbiyi, J. O., MacKay, E. H., Muronda, C. A., Alonge, 
T. O., Danso, A. P., ... & Wong, A. J. (2001). Further characterization of 
storage-related alterations in immunoreactivity of archival tissue sections and 
its implications for collaborative multicenter immunohistochemical studies. 
Applied Immunohistochemistry & Molecular Morphology, 9(3), 261-266.

Mirlacher, M., Kasper, M., Storz, M., Knecht, Y., Dürmüller, U., Simon, R., ... 
& Sauter, G. (2004). Influence of slide aging on results of translational 
research studies using immunohistochemistry. Modern pathology, 17(11), 1414.

DiVito, K. A., Charette, L. A., Rimm, D. L., & Camp, R. L. (2004). Long-term 
preservation of antigenicity on tissue microarrays. Laboratory investigation, 
84(8), 1071.

Fergenbaum, J. H., Garcia-Closas, M., Hewitt, S. M., Lissowska, J., Sakoda, L. 
C., & Sherman, M. E. (2004). Loss of antigenicity in stored sections of breast 
cancer tissue microarrays. Cancer Epidemiology and Prevention Biomarkers, 
13(4), 667-672.

Hameed, O., & Humphrey, P. A. (2009). Immunohistochemical evaluation of 
prostate needle biopsies using saved interval sections vs new recut sections 
from the block: a prospective comparison. American journal of clinical 
pathology, 131(5), 683-688.

Xie, R., Chung, J. Y., Ylaya, K., Williams, R. L., Guerrero, N., Nakatsuka, N., 
... & Hewitt, S. M. (2011). Factors influencing the degradation of archival 
formalin-fixed paraffin-embedded tissue sections. Journal of Histochemistry & 
Cytochemistry, 59(4), 356-365.

Seidu, M. A., Adams, A. R., Gyasi, R. K., Tettey, Y., Nkansah, D. O., & Wiredu, 
E. K. (2013). Immunoreactivity of some epitopes in longtime inappropriately 
stored paraffin-embedded tissues. Journal of Histotechnology, 36(2), 59-64.

Nuovo, A. J., Garofalo, M., Mikhail, A., Nicol, A. F., Vianna-Andrade, C., & 
Nuovo, G. J. (2013). The effect of aging of formalin-fixed paraffin-embedded 
tissues on the in situ hybridization and immunohistochemistry signals in 
cervical lesions. Diagnostic Molecular Pathology, 22(3), 164-173.

Grillo, F., Bruzzone, M., Pigozzi, S., Prosapio, S., Migliora, P., Fiocca, R., 
& Mastracci, L. (2017). Immunohistochemistry on old archival paraffin blocks: 
is there an expiry date?. Journal of Clinical Pathology, jclinpath-2017.

Giunchi, F., Degiovanni, A., Daddi, N., Trisolini, R., Dell'Amore, A., 
Agostinelli, C., ... & Fiorentino, M. (2018). Fading With Time of PD-L1 
Immunoreactivity in Non-Small Cells Lung Cancer Tissues: A Methodological 
Study. Applied Immunohistochemistry & Molecular Morphology, 26(7), 489-494.


-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, 17 August 2018 9:49 AM
To: HistoNet
Subject: [Histonet] Unstained slides - how long are they good for?

Hello My Fellow Histologists,

Happy Friday Eve.

The question has come up..  How long are *unstained* slides good for?
Not for H but tests like IHC and molecular testing.  These slides have been 
cut, stored at room temperature, not sealed in anyway, and kept in a cardboard 
box.

Please let me know what your opinions are and what your retention policy is 
concerning *unstained* slides.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



*Confidentiality Notice*: The information 

Re: [Histonet] Unstained slides - how long are they good for?

[Jamie Watson via Histonet]

It depends on the stability of the protein and fixation, some are stable 
for a week some for years. We use 6 months as a standard.

Jamie

On August 16, 2018 4:59:34 PM P Sicurello via Histonet 
 wrote:

> Hello My Fellow Histologists,
>
> Happy Friday Eve.
>
> The question has come up..  How long are *unstained* slides good for?
> Not for H but tests like IHC and molecular testing.  These slides have
> been cut, stored at room temperature, not sealed in anyway, and kept in a
> cardboard box.
>
> Please let me know what your opinions are and what your retention policy is
> concerning *unstained* slides.
>
> Thanks oodles.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material.  Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited.  If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




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[Histonet] Unstained slides - how long are they good for?

[P Sicurello via Histonet]

Hello My Fellow Histologists,

Happy Friday Eve.

The question has come up..  How long are *unstained* slides good for?
Not for H but tests like IHC and molecular testing.  These slides have
been cut, stored at room temperature, not sealed in anyway, and kept in a
cardboard box.

Please let me know what your opinions are and what your retention policy is
concerning *unstained* slides.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
___
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[Histonet] Recall: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA

[Morken, Timothy via Histonet]

Morken, Timothy would like to recall the message, "Histotechnologist, nights, 
Saturday thru Thursday, UCSF Medical Center, San Francisco, CA".
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[Histonet] FW: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA

[Morken, Timothy via Histonet]

A position is open for an night-shift histotech at UCSF, San Francisco. 
Saturdays thru Thursdays. Starting time is flexible between 10:00 pm and 1:00 AM

The Histology Lab at UCSF is a full service lab with routine, special stains, 
IHC and ISH. The workflow is fully barcoded and all equipment is state of the 
art. 

Pay ranges (hourly, depending on experience) with night shift differential (to 
be confirmed by HR on offer):

Histotech 1:   $41.07 to 51.16, $3.45
Histotech 2:  44.09 to 54.91, $4.50
Histotech 3:  48.16 to 60.07, $4.50

UCSF also offers extensive benefits:  
http://jobs.ucsfmedicalcenter.org/whychoose_employment.html


Apply at:
http://jobs.ucsfmedicalcenter.org/

Job ID is 16324

Contact Yuri Murphy, Histology Supervisor,  at  yuri.mur...@ucsf.edu with any 
questions.


Job Title

Histotechnologist - Pathology-Surgical/Histology (HISTO TCHNO 1, 2 OR 3)
Job ID  16324

Job Code   9065, 9066, 9067 
Job Family,  Technical & Technologist
Location:   Mount Zion (SF)
Weekly Hours:   40 :  100%
Appointment Type:   Career
Department:  Pathology-Surgical/Histology
Shift"  8-hour Nights
Full/Part Time:  Full-Time


Union Information:   This classification is represented by a union


Favorite Job


At UCSF Health, our mission of innovative patient care, advanced technology and 
pioneering research is redefining what's possible for the patients we serve - a 
promise we share with the professionals who make up our team. 
Ranked by U.S. News & World Report as the number one hospital in California - 
and among the top five in the country - UCSF Health is committed to providing 
the most rewarding work experience while delivering the best care available 
anywhere. In an environment that allows for continuous learning and 
opportunities for professional growth, UCSF Health offers the ideal atmosphere 
in which to best use your skills and talents.

Job Summary


Classification of Histotechnologist 1, 2, or 3 will be determined based upon 
the qualifications of the selected candidate.
Under supervision (HT-I, II level) by Senior-level technologists, Lead 
technologist and the Histology Supervisor, or direction (HT-III) by Lead 
technologist and the Histology Supervisor, or direction (HT-Lead) by the 
Histology Supervisor, the incumbent serves as a Histotechnologist in the 
Histology laboratory.
Duties include tissue processing, embedding, paraffin sectioning, H staining, 
Special Staining, specimen receipt and accessioning, Laboratory information 
system operation, Quality Assurance record keeping, instrument maintenance, 
intra-operative frozen sections, and other technical duties as assigned, 
including coverage in the Immunohistochemistry laboratory and Grossing lab as 
determined by the Lab Manager.
Rotates weekly between workstations within the lab. Work schedule is variable 
to include Saturdays and holiday coverage as scheduled. Incumbent must be able 
to flex work hours as needed to meet department operational needs and cover 
work rotations.

Required Qualifications


. HT 1:
o Associate degree or at least 60 semester hours of academic credit from a 
regionally accredited college/university, with a combination of 12 semester 
hours of biology and chemistry, AND one year of experience as a 
histotechnologist, preferably in a high-volume hospital histology laboratory 
within the last five years; or completion of an associate degree from an 
accredited Histotechnology program
o Excellent interpersonal and communication skills
. HT2:
o Current  HT license
o Associate degree or at least 60 semester hours of academic credit from a 
regionally accredited college/university, with a combination of 12 semester 
hours of biology and chemistry, AND two year of experience as a 
histotechnologist, in a high-volume hospital histology laboratory within the 
last five years; or completion of an associate degree from an accredited 
Histotechnology program
o Demonstrated high volume and high quality sectioning skills
o Excellent interpersonal and communication skills
. HT3:
o Current  HT license
o Associate degree or at least 60 semester hours of academic credit from a 
regionally accredited college/university, with a combination of 12 semester 
hours of biology and chemistry, AND four years of experience as a 
histotechnologist in a comparable high-volume hospital histology laboratory 
within the last five years
o Demonstrated high-volume, high-quality sectioning and staining skills
o Ability to organize and prioritize responsibilities and perform well under 
pressure to meet deadlines
o Excellent interpersonal and communication
. The flexibility to orient and work at all UCSF Medical Center locations

Preferred Qualifications


HT 1:
. One to two years experience as a Histotechnologist
. ASCP certification: HT or HTL licensed

HT2:
. One to two years experience as a Histotechnologist
. ASCP certified HTL

HT3:
. Five years experience as a Histotechnologist in a large hospital laboratory
. ASCP certified HTL

[Histonet] I have an open part time position

[Cazares, Ruth via Histonet]

Good morning Histonetters!

Swedish Covenant Hospital in Chicago has an available 20 hour per pay period 
position. The hours are 8 pm to midnight, 2 days one week and 3 days the next 
week.  The days are shared with a second histotech and the Fridays are 
alternated.  You would be responsible for cutting biopsies and any immunos, 
special stains or deepers ordered after the techs have left.

If anyone is interested, please apply by following the link below.

https://recruiting2.ultipro.com/SWE1003SCHMC/JobBoard/b3bbfb25-ba1f-43ae-a6cb-4f0e2cb70043/?q=hist=relevance

Any questions, please feel free to contact me directly.


Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 N. California
Chicago, IL 60625
(773) 878-8200 ext-5190
rcaza...@schosp.org


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[Histonet] Histology slide stainer questions

[Charles Riley via Histonet]

 Does anyone out there use the General Data Healthcare™ SHUR/Stain™ 3030
H Slide Stainer?If so can this machine be used to run PAP stains for
gyn and non-gyn specimens?  I am looking to replace my Gemini stainer and
routine H stainer with a single unit if possible to free up some lab
space.  If anyone has any other suggestions it would be greatly appreciated





Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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