Re: [Histonet] Unstained slides - how long are they good for?
I’m a histology workflow consultant that visits many AP laboratories each year. Almost every laboratory has a different retention policy. The average of most laboratories is to hold onto unstained slides for three weeks after final sign out. Typically the unstained slide can be held for a long period of time if used just for morphological staining. However if the unstained slides is going to be used for IHC or molecular testing, the antigenicity of the slide begins degrading at the point of cutting. Typically, however, for a high-quality IHC staining, if stored at room temperature, the unstained slide should not be held much longer than one month. And even at that time frame you will begin to see the degrading of the stain quality. If the slides are kept in a closed box, in refrigerator, they have longer retention. Typically up to 2 to 3 months. I hope this helps Sent from my iPhone > On Aug 16, 2018, at 19:48, P Sicurello wrote: > > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up.. How long are *unstained* slides good for? > Not for H but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 177, Issue 16
Decal Cal Stat company was sold to Stat Lab Medical Products. Last year -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Friday, August 17, 2018 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 177, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FW: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA (Morken, Timothy) 2. Recall: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA (Morken, Timothy) 3. Unstained slides - how long are they good for? (P Sicurello) 4. Re: Unstained slides - how long are they good for? (Jamie Watson) 5. Re: Unstained slides - how long are they good for? (Tony Henwood (SCHN)) 6. Decalcification for Bone Marrow Biopsies (Cartun, Richard) 7. Re: Unstained slides - how long are they good for? (Morken, Timothy) -- Message: 1 Date: Thu, 16 Aug 2018 22:12:38 + From: "Morken, Timothy" To: Histonet Subject: [Histonet] FW: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA Message-ID: Content-Type: text/plain; charset="iso-8859-1" A position is open for an night-shift histotech at UCSF, San Francisco. Saturdays thru Thursdays. Starting time is flexible between 10:00 pm and 1:00 AM The Histology Lab at UCSF is a full service lab with routine, special stains, IHC and ISH. The workflow is fully barcoded and all equipment is state of the art. Pay ranges (hourly, depending on experience) with night shift differential (to be confirmed by HR on offer): Histotech 1:?? $41.07 to 51.16, $3.45 Histotech 2:? 44.09 to 54.91, $4.50 Histotech 3:? 48.16 to 60.07, $4.50 UCSF also offers extensive benefits:? http://jobs.ucsfmedicalcenter.org/whychoose_employment.html Apply at: http://jobs.ucsfmedicalcenter.org/ Job ID is 16324 Contact Yuri Murphy, Histology Supervisor, ?at ?yuri.mur...@ucsf.edu with any questions. Job Title Histotechnologist - Pathology-Surgical/Histology (HISTO TCHNO 1, 2 OR 3) Job ID 16324 Job Code 9065, 9066, 9067 Job Family, Technical & Technologist Location: Mount Zion (SF) Weekly Hours: 40 :? 100% Appointment Type: Career Department: Pathology-Surgical/Histology Shift" 8-hour Nights Full/Part Time: Full-Time Union Information: This classification is represented by a union Favorite Job At UCSF Health, our mission of innovative patient care, advanced technology and pioneering research is redefining what's possible for the patients we serve - a promise we share with the professionals who make up our team.? Ranked by U.S. News & World Report as the number one hospital in California - and among the top five in the country - UCSF Health is committed to providing the most rewarding work experience while delivering the best care available anywhere. In an environment that allows for continuous learning and opportunities for professional growth, UCSF Health offers the ideal atmosphere in which to best use your skills and talents. Job Summary Classification of Histotechnologist 1,?2, or 3 will be determined based upon the qualifications of the selected candidate. Under supervision (HT-I, II level) by Senior-level technologists, Lead technologist and the Histology Supervisor, or direction (HT-III) by Lead technologist and the Histology Supervisor, or direction (HT-Lead) by the Histology Supervisor, the incumbent serves as a Histotechnologist in the Histology laboratory. Duties include tissue processing, embedding, paraffin sectioning, H staining, Special Staining, specimen receipt and accessioning, Laboratory information system operation, Quality Assurance record keeping, instrument maintenance, intra-operative frozen sections, and other technical duties as assigned, including coverage in the Immunohistochemistry laboratory and Grossing lab as determined by the Lab Manager. Rotates weekly between workstations within the lab. Work schedule is variable to include Saturdays and holiday coverage as scheduled. Incumbent must be able to flex work hours as needed to meet department operational needs and cover work rotations. Required Qualifications . HT 1: o Associate degree?or at least 60 semester hours of academic credit from a regionally accredited college/university, with a
Re: [Histonet] Unstained slides
I'm with Tim Morken on this one. The variability of antigenicity in storage is so wide open, and there really is no recent data, so we just make a point of educating our techs on not wasting tissue/levels during sectioning. If the techs feel that the residual tissue in the block is in danger of being exhausted, we communicate with our pathologists on how best to handle any requests. Unstained slides was time, money, and storage and we are better off without them. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 7. Re: Unstained slides - how long are they good for? (Morken, Timothy) Message: 7 Date: Fri, 17 Aug 2018 15:16:00 + From: "Morken, Timothy" To: P Sicurello Subject: Re: [Histonet] Unstained slides - how long are they good for? Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2018 4:49 PM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up.. How long are *unstained* slides good for? Not for H but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- End of Histonet Digest, Vol 177, Issue 16 * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides - how long are they good for?
Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2018 4:49 PM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up.. How long are *unstained* slides good for? Not for H but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Decalcification for Bone Marrow Biopsies
Help! For years we have used Decal-Stat for decalcifying our bone marrow biopsies with good results. For the past month we have been having problems with tissue loss and morphological damage with these specimens following decalcification. Unfortunately, this was just brought to my attention. Someone told me this morning that the company producing this product was sold and the formulation may have changed. Is that true? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
