Re: [Histonet] Unstained slides
I agree: cut only the sections needed. Saves space. Sure, you lose several sections of tissue when cutting more sections. That is acceptable because, if this "oxidation" theory is true, then the initial sections will be no good. However, careful organisation of exptl procedure before actual cutting will work very well. Actually, not many Ags get "oxidised"for eg: I can demonstrate GFAP in sections that are a year old ( sure, they are stored at 4C just in case) These slides are used for Yr 1 BSc practicals and are consistently positive. Nobody knows why some Ags ( and not others) lose their antigenicity, imho Oxidation is a vague reasoning. Just like nobody really knows why HIER works: however, I am in the dipole moment school of thought, rather than the Ca++ skool Sure, in Formalin-fixed specimens. Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides
On this issue of lost of antigenicity, never forget air oxygen!René
On Monday, September 3, 2018 11:11 AM, "Frazier, John"
wrote:
Interesting that you stated that, I was at the university of Colorado
this past week and was speaking with the medical director of the
pathology department. We actually started talking about unstained
slides and their storage conditions. We actually spoke of the histonet
discussions around unstained slide storage. He stated to me that due
to the elevation and lack of humidity in Denver that the antigenicity
of unstained slides has been up to multiple years. This is due to, as
you stated, water in the tissue.
Sent from my iPad
> On Sep 3, 2018, at 9:42 AM, Cartun, Richard
> wrote:
>
> It appears that the presence of water, both endogenously and exogenously,
> plays a central role in the loss of antigenicity in stored unstained slides
> (see reference below). Labs that are experiencing significant loss of
> immunoreactivity in their unstained slides should check their tissue
> processing.
>
> Xie R, Chung J-Y, Ylaya K, et al.: Factors influencing the degradation of
> archival formalin-fixed, paraffin-embedded tissue sections. J of Histochem
> Cytochem 2011; 59:356-365.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic
> Proteomics Laboratory
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 972-1596
> (860) 545-2204 Fax
>
> -Original Message-
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Sunday, August 19, 2018 2:09 PM
> To: Frazier, John; Terri Braud
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Unstained slides
>
> This is an email from Outside HHC. USE CAUTION opening attachments or links
> from unknown senders.
>
> Everything has been pointed out is correct BUT also pivot on "how the
> unstained slides are kept".Kept in a box their "useful life" is quite short
> (not beyond 1 week at the most).Kept at -80ºC I have used them after years of
> being stored the principle being of deep-freezing and this is "standard
> procedure" for IDF "+ controls".Kept in a Coplin jar filled with mineral oil
> or paraffin covered I have used them after months of being stored the
> principle being that, isolated from air oxygen, epitopes do not oxidize
> ("weaken") of if they do, the rate is greatly slowed.On the other hand,
> usually, unstained slides are kept for only few days in the event that, lets
> say within a week, the PT decides to order some special procedure and
> sometimes it is impossible "return" to the original block many times "almost
> exhausted".Properly done storing unstained slides are extremely useful.René
>
> On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet"
> wrote:
>
>
> I agree with Tim as well. This is what we advise our clients to do. It takes
> some coordination with the pathologist, but it is the best strategy for
> reducing unnecessary unstained slides. In the studies that we have performed
> only 10% of the unstained slides that are cut are you and the 90% are are it
> takes some coordination with the pathologist, but it is the best strategy for
> reducing unnecessary unstained slides. In the studies that we have performed
> only 10% of the unstained slides that are cut are you and 90% are thrown away
> thrown away.
> Several laboratories that I have visited in order to reduce the amount of
> wasted tissue when refacing the blocks, is to reseal the blocks with liquid
> paraffin, that have scant or small amounts of tissue in the block, such as
> the needle core biopsy.
> Bottom line on this issue is to educate the pathologist, and not water and
> stain slides except in rare occasions
>
> Sent from my iPhone
>
>> On Aug 17, 2018, at 14:07, Terri Braud wrote:
>>
>> I'm with Tim Morken on this one. The variability of antigenicity in storage
>> is so wide open, and there really is no recent data, so we just make a point
>> of educating our techs on not wasting tissue/levels during sectioning. If
>> the techs feel that the residual tissue in the block is in danger of being
>> exhausted, we communicate with our pathologists on how best to handle any
>> requests. Unstained slides was time, money, and storage and we are better
>> off without them.
>>
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Huntingdon Pike
>> Meadowbrook, PA 19046
>> ph: 215-938-3689
>> fax: 215-938-3874
>> Care, Comfort, and Heal
>>
>> Today's Topics:
>> 7. Re: Unstained slides - how long are they good for?
>> (Morken, Timothy)
>>
>> Message: 7
>> Date: Fri, 17 Aug 2018 15:16:00 +
>> From: "Morken, Timothy"
>> To: P Sicurello
>> Subject: Re: [Histonet] Unstained slides - how long are they good for?
>>
>>
>> Paula,
Re: [Histonet] Unstained slides
Interesting that you stated that, I was at the university of Colorado
this past week and was speaking with the medical director of the
pathology department. We actually started talking about unstained
slides and their storage conditions. We actually spoke of the histonet
discussions around unstained slide storage. He stated to me that due
to the elevation and lack of humidity in Denver that the antigenicity
of unstained slides has been up to multiple years. This is due to, as
you stated, water in the tissue.
Sent from my iPad
> On Sep 3, 2018, at 9:42 AM, Cartun, Richard
> wrote:
>
> It appears that the presence of water, both endogenously and exogenously,
> plays a central role in the loss of antigenicity in stored unstained slides
> (see reference below). Labs that are experiencing significant loss of
> immunoreactivity in their unstained slides should check their tissue
> processing.
>
> Xie R, Chung J-Y, Ylaya K, et al.: Factors influencing the degradation of
> archival formalin-fixed, paraffin-embedded tissue sections. J of Histochem
> Cytochem 2011; 59:356-365.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic
> Proteomics Laboratory
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 972-1596
> (860) 545-2204 Fax
>
> -Original Message-
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Sunday, August 19, 2018 2:09 PM
> To: Frazier, John; Terri Braud
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Unstained slides
>
> This is an email from Outside HHC. USE CAUTION opening attachments or links
> from unknown senders.
>
> Everything has been pointed out is correct BUT also pivot on "how the
> unstained slides are kept".Kept in a box their "useful life" is quite short
> (not beyond 1 week at the most).Kept at -80ºC I have used them after years of
> being stored the principle being of deep-freezing and this is "standard
> procedure" for IDF "+ controls".Kept in a Coplin jar filled with mineral oil
> or paraffin covered I have used them after months of being stored the
> principle being that, isolated from air oxygen, epitopes do not oxidize
> ("weaken") of if they do, the rate is greatly slowed.On the other hand,
> usually, unstained slides are kept for only few days in the event that, lets
> say within a week, the PT decides to order some special procedure and
> sometimes it is impossible "return" to the original block many times "almost
> exhausted".Properly done storing unstained slides are extremely useful.René
>
>On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet"
> wrote:
>
>
> I agree with Tim as well. This is what we advise our clients to do. It takes
> some coordination with the pathologist, but it is the best strategy for
> reducing unnecessary unstained slides. In the studies that we have performed
> only 10% of the unstained slides that are cut are you and the 90% are are it
> takes some coordination with the pathologist, but it is the best strategy for
> reducing unnecessary unstained slides. In the studies that we have performed
> only 10% of the unstained slides that are cut are you and 90% are thrown away
> thrown away.
> Several laboratories that I have visited in order to reduce the amount of
> wasted tissue when refacing the blocks, is to reseal the blocks with liquid
> paraffin, that have scant or small amounts of tissue in the block, such as
> the needle core biopsy.
> Bottom line on this issue is to educate the pathologist, and not water and
> stain slides except in rare occasions
>
> Sent from my iPhone
>
>> On Aug 17, 2018, at 14:07, Terri Braud wrote:
>>
>> I'm with Tim Morken on this one. The variability of antigenicity in storage
>> is so wide open, and there really is no recent data, so we just make a point
>> of educating our techs on not wasting tissue/levels during sectioning. If
>> the techs feel that the residual tissue in the block is in danger of being
>> exhausted, we communicate with our pathologists on how best to handle any
>> requests. Unstained slides was time, money, and storage and we are better
>> off without them.
>>
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Huntingdon Pike
>> Meadowbrook, PA 19046
>> ph: 215-938-3689
>> fax: 215-938-3874
>> Care, Comfort, and Heal
>>
>> Today's Topics:
>> 7. Re: Unstained slides - how long are they good for?
>> (Morken, Timothy)
>>
>> Message: 7
>> Date: Fri, 17 Aug 2018 15:16:00 +
>> From: "Morken, Timothy"
>> To: P Sicurello
>> Subject: Re: [Histonet] Unstained slides - how long are they good for?
>>
>>
>> Paula, since it is variable we strive to not have unstained slides. We had
>> kept them indefinitely, then when storage was overwhelming us we
Re: [Histonet] Unstained slides
It appears that the presence of water, both endogenously and exogenously, plays
a central role in the loss of antigenicity in stored unstained slides (see
reference below). Labs that are experiencing significant loss of
immunoreactivity in their unstained slides should check their tissue processing.
Xie R, Chung J-Y, Ylaya K, et al.: Factors influencing the degradation of
archival formalin-fixed, paraffin-embedded tissue sections. J of Histochem
Cytochem 2011; 59:356-365.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596
(860) 545-2204 Fax
-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Sunday, August 19, 2018 2:09 PM
To: Frazier, John; Terri Braud
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unstained slides
This is an email from Outside HHC. USE CAUTION opening attachments or links
from unknown senders.
Everything has been pointed out is correct BUT also pivot on "how the unstained
slides are kept".Kept in a box their "useful life" is quite short (not beyond 1
week at the most).Kept at -80ºC I have used them after years of being stored
the principle being of deep-freezing and this is "standard procedure" for IDF
"+ controls".Kept in a Coplin jar filled with mineral oil or paraffin covered I
have used them after months of being stored the principle being that, isolated
from air oxygen, epitopes do not oxidize ("weaken") of if they do, the rate is
greatly slowed.On the other hand, usually, unstained slides are kept for only
few days in the event that, lets say within a week, the PT decides to order
some special procedure and sometimes it is impossible "return" to the original
block many times "almost exhausted".Properly done storing unstained slides are
extremely useful.René
On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet"
wrote:
I agree with Tim as well. This is what we advise our clients to do. It takes
some coordination with the pathologist, but it is the best strategy for
reducing unnecessary unstained slides. In the studies that we have performed
only 10% of the unstained slides that are cut are you and the 90% are are it
takes some coordination with the pathologist, but it is the best strategy for
reducing unnecessary unstained slides. In the studies that we have performed
only 10% of the unstained slides that are cut are you and 90% are thrown away
thrown away.
Several laboratories that I have visited in order to reduce the amount of
wasted tissue when refacing the blocks, is to reseal the blocks with liquid
paraffin, that have scant or small amounts of tissue in the block, such as the
needle core biopsy.
Bottom line on this issue is to educate the pathologist, and not water and
stain slides except in rare occasions
Sent from my iPhone
> On Aug 17, 2018, at 14:07, Terri Braud wrote:
>
> I'm with Tim Morken on this one. The variability of antigenicity in storage
> is so wide open, and there really is no recent data, so we just make a point
> of educating our techs on not wasting tissue/levels during sectioning. If
> the techs feel that the residual tissue in the block is in danger of being
> exhausted, we communicate with our pathologists on how best to handle any
> requests. Unstained slides was time, money, and storage and we are better
> off without them.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Today's Topics:
> 7. Re: Unstained slides - how long are they good for?
> (Morken, Timothy)
>
> Message: 7
> Date: Fri, 17 Aug 2018 15:16:00 +
> From: "Morken, Timothy"
> To: P Sicurello
> Subject: Re: [Histonet] Unstained slides - how long are they good for?
>
>
> Paula, since it is variable we strive to not have unstained slides. We had
> kept them indefinitely, then when storage was overwhelming us we reduced it
> to 2 months maximum. Now we require request for unstained to be ordered in
> the system and delivered to the pathologist. We do not hold any in the lab.
> We recut when new stains are ordered. In the past we had routinely cut extras
> "just in case" but ended up with thousands of unstained slides that were
> never used. Instead we trained everyone to reduce wastage and get good
> sections from a cut block with minimal facing. We have not stored unstained
> sections for many years and they do not seem to be missed.
>
> Tim Morken
> Pathology Site Manager, Parnassus
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology UC San Francisco Medical Center
>
>
> -Original Message-
>
[Histonet] Grossing room design
Hi there, we are in the process of getting a new grossing room and I was wondering if there is any document or similar where the basic design requirements are stated. Something like the ventilation, material of the walls, requirements for electricity standards, pipes. Any suggestions/ideas/experience would be much appreciated! Thanks a lot for your help!! Julio -- Julio Benavides Silván Instituto de Ganadería de Montaña (CSIC-Universidad de León) 24346. Grulleros, León +34 987317156 ext.861162 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
