[Histonet] Seeking On call Histotech

[Heather Marlatt via Histonet]

Hello Histonet!

We are seeking an “on call” histotech to help us through our busy season
and potentially longer. Our lab is located in Spokane Valley, Wa and it’s a
fun relaxed environment. The schedule is very flexible and pay negotiable.
We are a veterinary and research focused lab but welcome all backgrounds.
It’s a very unique position with a lot of growth opportunity.

Please send resumes to nationwidehistol...@gmail.com

Thank you,
Heather Marlatt
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Re: [Histonet] Unstained slides

[Ana Maluenda via Histonet]

Hi all,

It has been very interesting reading all your comments on unstained slides. 
This has been a forever discussion I always have everywhere I go in different 
research institutes. So expanding the topic, I wonder what's everyone's opinion 
on unstained cryosections? How long are they reliable to be used for IHC?

Kind regards,

Ana

Ana Maluenda
Research Assistant
Atherothrombosis and Vascular Biology Laboratory

Baker Heart and Diabetes Institute
75 Commercial Road, Melbourne VIC 3004
P (03) 8532 1359 E ana.malue...@baker.edu.au W www.baker.edu.au


-Original Message-
From: Hobbs, Carl [mailto:carl.ho...@kcl.ac.uk]
Sent: Tuesday, 4 September 2018 4:54 AM
To: histonet 
Subject: Re: [Histonet] Unstained slides

I agree: cut only the sections needed.
Saves space.
Sure, you lose several sections of tissue when cutting more sections.
That is acceptable because, if this "oxidation" theory is true, then the 
initial sections will be no good.
However, careful organisation of exptl procedure before actual cutting will 
work very well.

Actually, not many Ags get "oxidised"for eg: I can demonstrate GFAP in 
sections that are a year old ( sure, they are stored at 4C just in case) These 
slides are used for Yr 1 BSc practicals and are consistently positive.
Nobody knows why some Ags ( and not others) lose their antigenicity, imho 
Oxidation is a vague reasoning.
Just like nobody really knows why HIER works: however, I am in the dipole 
moment school of thought, rather than the Ca++ skool Sure, in Formalin-fixed 
specimens.

Curious-illy

Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813

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[Histonet] HIF-1alpha and EBNA1

[Victoria Baker via Histonet]

To All,

I am seaching for a lab that can run both of these antibodies on human FFPE
tissue.

HIF-1 alpha is Hypoxia Inducible 1 alpha
EBNA1 is Epstein Barr Nuclear Antigen 1

If anyone performs these tests or knows of a lab that does, please contact
me.

Thank you in advance

Vikki
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