Re: [Histonet] Elephant Tissues

[Histology via Histonet]

Hi Jennifer,

If it is skin extending the fixing and processing time should cure any 
processing issues. If after this it’s still not working a short pre fix in 
khales fixative and surface softening before sectioning with 0.02% sodium 
hydroxide may help.

Hope this helps!

Stuart Beaver BSc(Hons)
Head of Veterinary Histology/Cytology
+447568543761

On 5 Sep 2018, at 22:46, P Sicurello 
mailto:pat...@gmail.com>> wrote:

Jennifer,

I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale,
giraffe, and even human mummy tissues.  With the exception of the mummy
tissue being a bit dry, they all embedded and cut like human tissue.

What is it that is making them hard to cut?

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.


On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet <
histonet@lists.utsouthwestern.edu> 
wrote:

Hello Histonetters,
Does anyone have experience processing and cutting elephant tissues?  Any
tips, tricks, or advice?  My lab has had some elephant cases recently and
the tissues are unexpectedly (to us) difficult to cut.

Thanks for any help,
Jennifer Phinney QIHC
Kansas State University
Veterinary Diagnostic Lab
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Pregnancy guide for working in histology

[Katelin Tellechea via Histonet]

Hi Carol,
I did a poster presentation on this topic at the NSH Symposium in Long
Beach, 2016.
I would be happy to send you a copy of my poster. I also wrote a blog
article about my research for the poster found here:
https://www.fixationonhistology.com/home/my-pregnancy-in-the-lab-researching-safety-considerations
The Block also has the abstract, podcast interview, and a Safety Snippet of
my poster.
Please contact me directly if you have any other questions or I can help in
any way.
Katelin


On Thu, Sep 6, 2018, 10:01 AM Carol Torrence via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Good morning!
>
> Could some of you chime in on guidelines you go by for those employee that
> are expecting a baby.  I have removed employee from xylene exposure during
> staining and cover slipping but am on the fence about grossing.  At this
> time the employee has been removed from grossing.  All grossing is done
> under an exhaust hood.  Our exposure badges have always read well below
> limits.  Thanks in advance!
>
> Carol M. Torrence, HT(ASCP)
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Paraffin Dispenser

[Laurie Colbert via Histonet]

I'm looking for a 2.5 gallon (approx) paraffin dispenser ASAP - new or 
refurbished.  I need to find one that is in stock and can be shipped 
immediately.  Vendors are welcome to respond.

Thank you,
Laurie Redmond
Path MD 
Los Angeles, CA
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Giemsa vs Diff Quik

[Bob Richmond via Histonet]

Joseph A. Esposito at McClain Laboratories on Long Island asks:

>>The laboratory I work at has been using the Diff Quik for years now as a
stain for fine needle aspirates. Recently, when we tried to reorder a Diff
Quik stain kit from our usual suppliers, we have found it to be on
backorder. This has caused us to begin to consider the Giemsa stain as an
alternative to the Diff Quik. Does anyone have any experience with using
both stains and how they compare to each other? Would the Giemsa be a
suitable alternative to replace the Diff Quik?<<

Diff-Quik® (spelt like that!) is an old registered trademark for a rapid
two-step Romanowsky stain. A number of Web sites inform me today that this
trade name has been discontinued, I suppose because the name has been so
commonly used generically.

There have long been a number of generic fast two-step Romanowsky stains
available, with a xanthene dye (presumably eosin Y) as solution 1, and a
proprietary mixture of thiazine dyes as solution 2. In my experience with
at least five of these, they all worked pretty much indistinguishably from
brand-name Diff-Quik.

There are a number of stains called Giemsa, also Romanowsky stains, but
often slower than the "quick" stains. There is probably no advantage to
using them for any reason.

In my eightieth year, I remember with nostalgia the old Wolbach Giemsa
technique for tissue sections. Tissue must be fixed in a dichromate
fixative, traditionally Zenker/Helly, sections stained in four successive
Giemsa baths, the last one overnight, and differentiated with 10%
colophonium rosin in alcohol with microscopic control. (I've actually done
this stain myself.)

Bob Richmond
Samurai Pathologist
Maryville TN
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Pregnancy guide

[Terri Braud via Histonet]

Remember that contamination can also be by skin absorption.  With that in mind, 
we provide thick Nitrile gloves with instruction to change them at least every 
30 minutes, or less if they show any signs of deterioration.  We have great 
ventilation and beyond that, when we wear our fume badges, we perform the worst 
exposure tasks, so when calculated, the exposure shows what it would be like if 
we did that task for 8 hours a day - and we STILL are way under the limit. The 
result: No restrictions of duties - 5 babies born to employees here without a 
single problem in 12 years.
However, with that said, I would never ask a pregnant technician to hand 
coverslip more than the occasional slide.
Just my 2cents - T

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
  

 2. Pregnancy guide for working in histology (Carol Torrence)
Message: 2
Date: Thu, 6 Sep 2018 16:43:47 +
From: Carol Torrence 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Pregnancy guide for working in histology
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Good morning!

Could some of you chime in on guidelines you go by for those employee that are 
expecting a baby.  I have removed employee from xylene exposure during staining 
and cover slipping but am on the fence about grossing.  At this time the 
employee has been removed from grossing.  All grossing is done under an exhaust 
hood.  Our exposure badges have always read well below limits.  Thanks in 
advance!

Carol M. Torrence, HT(ASCP)



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Pregnancy guide for working in histology

[Rene J Buesa via Histonet]

Both formalin and xylene (and any other dangerous fumes) have to be avoided 
during pregnancy BUT if you have an efficient fumes hood to do grossing, then 
you should monitor exposure. Somebody NOT pregnant should gross with a personal 
formalin badge and, depending on the exposure result, then you may allow the 
pregnant employee to do grossing or not.René 

On Thursday, September 6, 2018 1:08 PM, Carol Torrence via Histonet 
 wrote:
 

 Good morning!

Could some of you chime in on guidelines you go by for those employee that are 
expecting a baby.  I have removed employee from xylene exposure during staining 
and cover slipping but am on the fence about grossing.  At this time the 
employee has been removed from grossing.  All grossing is done under an exhaust 
hood.  Our exposure badges have always read well below limits.  Thanks in 
advance!

Carol M. Torrence, HT(ASCP)

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


   
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Pregnancy guide for working in histology

[Carol Torrence via Histonet]

Good morning!

Could some of you chime in on guidelines you go by for those employee that are 
expecting a baby.  I have removed employee from xylene exposure during staining 
and cover slipping but am on the fence about grossing.  At this time the 
employee has been removed from grossing.  All grossing is done under an exhaust 
hood.  Our exposure badges have always read well below limits.  Thanks in 
advance!

Carol M. Torrence, HT(ASCP)

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Elephant skin

[Terri Braud via Histonet]

Probably one of those things best embedded/cut in plastic.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Giemsa vs Diff Quik

[Mike Toole via Histonet]

For ordering the Diff-Quik alternative this link works better. It takes you 
directly to the manufacturer EKI.

https://eki-chem.com/product/2295

The labsco link below goes to McKesson.

Mike

-Original Message-
From: Mike Toole via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 06, 2018 10:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Giemsa vs Diff Quik

I ordered this a few weeks ago:   http://www.labsco.com/items/EKI2295 

STAIN BRI KT 16OZ KT 16OZ / 500ML DIFFERENTIAL RAPID BLOOD STAIN SOLUTION KIT, 
FOR DIFFERENTIAL STAINING OF BLOOD SMEARS, INCLUDES: XANTHENE DYE SOLUTION (A), 
THIAZINE DYE SOLUTION (B), FIXATIVE SOLUTION (C), 3 X 16OZ HAZMAT Item Id: 
EKI2295 

Supplier Part Number 2295-500ML Supplier Name E K INDUSTRIES, INC. Stock 14 
Availability Item in stock. Ships within one business day.

Mike

-Original Message-
From: Laurie Colbert via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 06, 2018 10:01 AM
To: jose...@mcclainlab.com; histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Giemsa vs Diff Quik

We use the Diff Quik in place of the Giemsa stain for H. Pylori on gastric 
bx's.  I believe you can use the Diff Quik for FNA's, especially if it is just 
for a preliminary diagnosis and you will be staining with H&E or a cytology 
stain later.  We get our Diff Quik from Medical Chemical Corporation.

Laurie Redmond


-Original Message-
From: Joseph Esposito via Histonet 
To: histonet 
Sent: Thu, Sep 6, 2018 7:42 am
Subject: [Histonet] Giemsa vs Diff Quik

The laboratory I work at has been using the Diff Quik for years now as a stain 
for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain 
kit from our usual suppliers, we have found it to be on backorder. This has 
caused us to begin to consider the Giemsa stain as an alternative to the Diff 
Quik. Does anyone have any experience with using both stains and how they 
compare to each other? Would the Giemsa be a suitable alternative to replace 
the Diff Quik?


Joseph A. Esposito
McClain Laboratories, LLC.
(631) 361- 4000


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Giemsa vs Diff Quik

[Mike Toole via Histonet]

I ordered this a few weeks ago:   http://www.labsco.com/items/EKI2295 

STAIN BRI KT 16OZ KT 16OZ / 500ML DIFFERENTIAL RAPID BLOOD STAIN SOLUTION KIT, 
FOR DIFFERENTIAL STAINING OF BLOOD SMEARS, INCLUDES: XANTHENE DYE SOLUTION (A), 
THIAZINE DYE SOLUTION (B), FIXATIVE SOLUTION (C), 3 X 16OZ HAZMAT Item Id: 
EKI2295 

Supplier Part Number 2295-500ML Supplier Name E K INDUSTRIES, INC. Stock 14 
Availability Item in stock. Ships within one business day.

Mike

-Original Message-
From: Laurie Colbert via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 06, 2018 10:01 AM
To: jose...@mcclainlab.com; histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Giemsa vs Diff Quik

We use the Diff Quik in place of the Giemsa stain for H. Pylori on gastric 
bx's.  I believe you can use the Diff Quik for FNA's, especially if it is just 
for a preliminary diagnosis and you will be staining with H&E or a cytology 
stain later.  We get our Diff Quik from Medical Chemical Corporation.

Laurie Redmond


-Original Message-
From: Joseph Esposito via Histonet 
To: histonet 
Sent: Thu, Sep 6, 2018 7:42 am
Subject: [Histonet] Giemsa vs Diff Quik

The laboratory I work at has been using the Diff Quik for years now as a stain 
for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain 
kit from our usual suppliers, we have found it to be on backorder. This has 
caused us to begin to consider the Giemsa stain as an alternative to the Diff 
Quik. Does anyone have any experience with using both stains and how they 
compare to each other? Would the Giemsa be a suitable alternative to replace 
the Diff Quik?


Joseph A. Esposito
McClain Laboratories, LLC.
(631) 361- 4000


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Stain removal on clothing

[Eileen Akemi Allison via Histonet]

Just found this info on-line for stain removal.  I assume it originally came 
from Chuck Churukian since it’s from University of Rochester.


REMOVING STAINS FROM HANDS AND CLOTHING

Some stains can be removed form the hands by using *Erado-Sol.  Dilute Chlorox 
(or similar bleach) and Erado-Sol are useful for removing stains from white 
clothing.  Care should be exercised in removing stains from colored clothing.

To remove the following stains use the solutions indicated.

1.Alcian blue - to remove from the hands use the acid alcohol solution. 
 For cleaning glassware try using dilute Chlorox or acid alcohol.  If the stain 
resists, use them alternatively.
2.Auramine-rhodamine - acid alcohol.
3.Basic fuchsin - Erado-Sol.
4.Carmine - acid alcohol.
5.Chromic acid - concentrated aqueous sodium thiosulfate containing a 
few drops of sulfuric acid.
6.Crystal violet - Erado-Sol or Chlorox.
7.Eosin Y - saturated aqueous lithium carbonate.
8.Fast green and similar acid dyes - saturated aqueous lithium 
carbonate.
9.Hematoxylin (alum and iron) - Erado-Sol or acid alcohol.
10.  Iodine - 5% sodium thiosulfate.
11.  Methylene blue - acid alcohol, Erado-Sol or Chlorox.
12.  Nuclear fast red - acid alcohol.
13.  Picric acid - saturated aqueous lithium carbonate.
14.  Potassium permanganate - 5% oxalic acid.
15.  Schiffs solution - Erado-Sol or Chlorox.
16.  Silver deposits - Lugols iodine followed by 5% sodium thiosulfate.
17.  Sudan stains (Oil red O, Sudan black B) - acetone.
18.  Thioflavin T - acid alcohol.


*American Scientific Products
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Giemsa vs Diff Quik

[Laurie Colbert via Histonet]

We use the Diff Quik in place of the Giemsa stain for H. Pylori on gastric 
bx's.  I believe you can use the Diff Quik for FNA's, especially if it is just 
for a preliminary diagnosis and you will be staining with H&E or a cytology 
stain later.  We get our Diff Quik from Medical Chemical Corporation.

Laurie Redmond


-Original Message-
From: Joseph Esposito via Histonet 
To: histonet 
Sent: Thu, Sep 6, 2018 7:42 am
Subject: [Histonet] Giemsa vs Diff Quik

The laboratory I work at has been using the Diff Quik for years now as a stain 
for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain 
kit from our usual suppliers, we have found it to be on backorder. This has 
caused us to begin to consider the Giemsa stain as an alternative to the Diff 
Quik. Does anyone have any experience with using both stains and how they 
compare to each other? Would the Giemsa be a suitable alternative to replace 
the Diff Quik?


Joseph A. Esposito
McClain Laboratories, LLC.
(631) 361- 4000


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Elephant Tissues

[Jennifer Phinney via Histonet]

Hi Paula,
One of my pathologists thinks it could be the collagen in the tissues making 
them difficult to cut.  We have tried nair, fabric softener, and even decaling 
the tissues and nothing helps.  The tissues shred immediately when trying to 
section making it impossible in some cases to actually get a slide. When I 
melted a particularly difficult block down to separate out the different 
tissues, it turned out to be the lymph node that was the worst.

We are going to try some of the cream you use to soften scar tissues to see if 
that has any effect.

As a veterinary histology lab we routinely work on a variety of different 
species, and so far the elephant tissue is the only one giving us problems.

Thanks for everyone’s help,
Jennifer

From: P Sicurello 
Sent: Wednesday, September 5, 2018 4:46 PM
To: Jennifer Phinney 
Cc: HistoNet 
Subject: Re: [Histonet] Elephant Tissues

Jennifer,

I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale, 
giraffe, and even human mummy tissues.  With the exception of the mummy tissue 
being a bit dry, they all embedded and cut like human tissue.

What is it that is making them hard to cut?

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



Confidentiality Notice: The information transmitted in this e-mail is intended 
only for the person or entity to which it is addressed and may contain 
confidential and/or privileged material.  Any review, retransmission, 
dissemination or other use of or taking of any action in reliance upon this 
information by persons or entities other than the intended recipient is 
prohibited.  If you received this e-mail in error, please contact the sender 
and delete the material from any computer.


On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:
Hello Histonetters,
Does anyone have experience processing and cutting elephant tissues?  Any tips, 
tricks, or advice?  My lab has had some elephant cases recently and the tissues 
are unexpectedly (to us) difficult to cut.

Thanks for any help,
Jennifer Phinney QIHC
Kansas State University
Veterinary Diagnostic Lab
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Giemsa vs Diff Quik

[Joseph Esposito via Histonet]

The laboratory I work at has been using the Diff Quik for years now as a stain 
for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain 
kit from our usual suppliers, we have found it to be on backorder. This has 
caused us to begin to consider the Giemsa stain as an alternative to the Diff 
Quik. Does anyone have any experience with using both stains and how they 
compare to each other? Would the Giemsa be a suitable alternative to replace 
the Diff Quik?


Joseph A. Esposito
McClain Laboratories, LLC.
(631) 361- 4000


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet