[Histonet] H for H. pylori

[Cassie P. Davis via Histonet]

In the beginning of my last FT job we had a excellent female pathologist who 
could see the H. pylori on SurgiPath's  H stain. I learned so much from her 
and dearly missed her toward the end.

Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  cda...@che-east.org



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[Histonet] Large bone samples

[Mike Tighe via Histonet]

Hi Everyone,


I am looking into doing some histology (HandE) on NHP joints. Samples will be 
finger joints and wrist. If you have any experience with these larger bones 
(and soft tissue) I would be interested in any advice on general time and type 
of decal solution. I only need to do H no immuno so I think acid decal is OK.


Thanks for any help!!

Mike
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Re: [Histonet] Leica Bond Max artifact

[Cathy M. Mathis/Comparative Medicine via Histonet]

Hello Dr. Rosen and Histonetters,
We have used a Leica Bond RX for over 2 years now, cleaning and caring for it 
just as you do.  My work is mostly animal which is why we mainly choose the red 
chromogen alkaline phosphatase detection kit.  We see the same artifact 
sporadically.  Sometimes we will see it for several days and then maybe a week 
goes by and we don't see any of it.  It may only be a small amount and I have 
seen it basically all over the slide.  I know it is not related to our tissues 
or slides.  Our Leica rep came out and increased washes but it really doesn't 
help.  If anyone has a solution to the problem, I would also be interested.
Best to all,
Cathy

Cathy M. Mathis
Laboratory Technician IV
Department of Pathology 
Section on Comparative Medicine - Clarkson Campus
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.1538 \  f 336.716.1515  
cmmat...@wakehealth.eduwakehealth.edu


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Sent: Wednesday, October 10, 2018 1:00 PM
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Subject: Histonet Digest, Vol 179, Issue 9

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Today's Topics:

   1. FW: Leica Bond Max artifact (Linda Margraf)
   2. Re: FW: Leica Bond Max artifact (Rene J Buesa)
   3. H for H. pylori? (P Sicurello)
   4. Re: H for H. pylori? (Rene J Buesa)
   5. Re: H for H. pylori? (Tony Henwood (SCHN))
   6. Job Opening in Massachusetts: IHC Lab Supervisor (Erin Sarricks)
   7. Low Oxygen Sensor/Alarms (Cooper, Brian)


--

Message: 1
Date: Tue, 9 Oct 2018 17:06:27 +
From: Linda Margraf 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] FW: Leica Bond Max artifact
Message-ID: 
Content-Type: text/plain; charset="utf-8"

Here is a message I am posting for Dr. Rosen:
Hello,

I am a longtime admirer of this list, and I am sure someone on here can help us 
out

We have an artifact on our immunohistochemistry (IHC) slides.

I will attempt to attach pictures using the upload tool. In case that doesn?t 
work, I am attaching a google drive link to some jpgs. The artifact is usually 
golden brown to red/pink in color, is clustered, appears both on top of the 
tissue and around it. If we do an aggressive rundown, it appears less red/brown 
and bluer.

We are using a Leica Bond MAX IHC system which is about a year old. We usually 
do two runs a day and 95% of the time we only use a red detection kit. We only 
see this on our red detection kit on our Leica bond. The brown is unaffected. 
The corresponding H slides are fine.
We started having this artifact after a month or two of production. 85% of the 
time we have some sort of artifact. Sometimes its perfect, sometimes it makes 
the tissue unreadable. We haven?t figured out any precipitating factors.

We have tried a number of things, but we still can?t get control of this.
Here is a brief overview of our protocols:
- Processor: Leica ASP300s tissue processor.
-Parrafin = Mercedes HWWX (dual purpose embedding and infiltration parrafin) 
melting point 57c ? we tried switching, and it didn?t help.
- We only use distilled water in our baths
- Mercedes Starfrost charged slides
- Oven for 30 min at 62c
- We always use a cold red detection kit
-Sometimes our runs are delayed but not more than 4 hours
- Rundown is done in our stainer (standardized), and slides are coverslipped 
with tape.
- The bulk fluid containers in the bond are cleaned weekly and refrigerated 
between runs.
- The probe is cleaned every 300 slides.
- Mixing wells are cleaned out every two weeks and replaced often.
-Covertiles are well tended to.

We have tried to correct this problem, but we can't seem to fix it.
Any ideas?