Re: [Histonet] Inconsistent H & E Staining
Have you checked your dewaxing solutions? I have sometimes seen this when slides are not fully dewaxed. yours, mills ᐧ On Wed, Nov 14, 2018 at 12:34 PM MONICA D. LOCKHART via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello Histo Fam!! > > Please help! > > My facility is experiencing frequent H & E staining problems. Everything > from cloudy cells on routine GI to "The Blank Look" on PNB's. > > We have made changes in some of the obvious areas like cassette whole size > and discontinued the use of sponges at grossing. However, with our > biopsies, we still see varying results in our H staining. > > Though this is not an exhaustive list of all the things we have > encountered or the number of changes we have made, hopefully this will give > you an idea of what we are seeing and maybe you have ran across this > problem and can share your wisdom. > > Any comments would be appreciated and most welcome. > > > Monica D. Lockhart, BBA, HT (ASCP) PBT > Supervisor Clinical Labs Histology > Loyola University Medical Center > 2160 S. First Ave, Bldg 110 Rm 2290 > Maywood, IL 60153 > (o) 708.327.2608 > (c) 708.692.8361 > monica.lockh...@luhs.org > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help with frozen spleen and liver tissue!
Hi all, Can someone please provide me with more details regarding cryosectioning of mouse spleen and liver tissue? Currently, I've been fixing my samples in 4% PFA (they are well fixed - I know that will be the first question asked!), and then cryoprotect the tissue in a series of graded sucrose solutions (15% to 30%) until they sink. However, when I go to place the sections on the slide from the cryostat, they look great initially under the microscope, but once they dry they have poor morphology, especially seen in the liver. I've never run into this issue before, with either brain or spinal cord which are significantly more delicate. I've tried cutting the tissue free-floating as well to see if it was how I was placing the sections on the slide, but nothing seems to work. The morphology in the liver is so terribly compromised that I cannot visualize the sinusoids properly. It is baffling that once they go onto the slide they look okay, but 5 minutes later the tissue appears to "separate" from itself. Does anyone work specifically in frozen tissue sections, liver and spleen in particular? If so, would you be able to help me figure out the best way to generate quality specimens? Thank you! Regards, Allyse Mazzarelli ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Inconsistent H & E Staining
Hello Histo Fam!! Please help! My facility is experiencing frequent H & E staining problems. Everything from cloudy cells on routine GI to "The Blank Look" on PNB's. We have made changes in some of the obvious areas like cassette whole size and discontinued the use of sponges at grossing. However, with our biopsies, we still see varying results in our H staining. Though this is not an exhaustive list of all the things we have encountered or the number of changes we have made, hopefully this will give you an idea of what we are seeing and maybe you have ran across this problem and can share your wisdom. Any comments would be appreciated and most welcome. Monica D. Lockhart, BBA, HT (ASCP) PBT Supervisor Clinical Labs Histology Loyola University Medical Center 2160 S. First Ave, Bldg 110 Rm 2290 Maywood, IL 60153 (o) 708.327.2608 (c) 708.692.8361 monica.lockh...@luhs.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] BioCare mart 1
Just call BioCare Technical service. They have a complete procedure for hand staining. I know because I used it in their facility when I went for training on their Flx. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 3. MART-1 immunot procedure (Katie) -- Message: 3 Date: Wed, 14 Nov 2018 09:16:58 -0800 From: "Katie" Subject: [Histonet] MART-1 immunot procedure Hello all, My dermpath lab is looking into adding a MART-1 immuno, as a hand stain. The procedure that I received from BioCare for their cocktail kit is extremely minimal. Do any of you have a more thorough and/or broken down hand stain SOP that I could please reference? Thank you so much, Katie Riley-Hamilton Technical Supervisor of Dermatopathology Puyallup Dermatology Clinic ka...@puyallupderm.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MART-1 immunot procedure
Hello all, My dermpath lab is looking into adding a MART-1 immuno, as a hand stain. The procedure that I received from BioCare for their cocktail kit is extremely minimal. Do any of you have a more thorough and/or broken down hand stain SOP that I could please reference? Thank you so much, Katie Riley-Hamilton Technical Supervisor of Dermatopathology Puyallup Dermatology Clinic ka...@puyallupderm.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Posting for a friend! Histopeeps!! Know any Cytopeeps looking for some Temp Work?
Hi Pam, Thanks for your help with this. See below. Please copy and paste. Full Time temporary Cytotech Pathologists Diagnostic Laboratory a private pathology laboratory located in downtown Winston Salem, is currently seeking a Cytotech to fill a temporary full-time position. This is a first shift position Monday to Friday with hours that are negotiable. Timeframe 8-10 weeks (Dec17 thru Feb 18) General Responsibilities Screening Gyn/Non-Gyn specimens Accession/Process Gyn samples Perform molecular testing using automated analyzer (training provided) Maintain workload records Qualifications CT ASCP certified Molecular experience preferred, not required 1-3 years experience For more information contact: Cheryl Morrison Human Resources Manager Pathologists Diagnostic Laboratory, PA (336)306-5770 cpmorri...@pdlpath.com Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] TMAs for MMR validation
Will the US Biomax TMA CO804A show at least 10 cores with loss of staining for the MMR panel? Thanks! Jacquilyn R. Vergara MLS, HTL (ASCP)cm Histotechnologist/QA Coordinator MicroPath Laboratories, Inc. 1125 Bartow Rd., Ste. 101 Lakeland, FL 33801 WP:863-577-1147 NOTICE OF CONFIDENTIALITY The information in this email, including attachments, may be confidential and/or privileged and may contain confidential health information. This email is intended to be reviewed only by the individual or organization named as addressee. If you have received this email in error please notify MicroPath Laboratories immediately by return message to the sender or to i...@micropathlabs.com. Destroy all copies of this message and any attachments. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of MicroPath Laboratories. Confidential health information is protected by state and federal law, including, but not limited to: The Health Insurance Portability and Accountability Act of 1996 and related regulations. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
