Possibly, the edges have been allowed to dry prior to immersion in fixative.
Also is there evidence of cautery artefact? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Debra Siena via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Saturday, 9 February 2019 3:51 AM To: 'histonet' Subject: [Histonet] Derm IHC question Hello fellow Histonetters I would like to ask you a question about IHC staining and derm cases. I am seeing a peculiar issue going on, where the melanocytes in the middle of the tissues are staining pretty well but when you get to the ends of the tissues either shaves or ellipses, they are not staining. This is sporadic, not every case and there is no consensus as to a common thread between the cases. I feel that this may be a fixation issue but was just wondering if anyone had ever seen the same phenomena and would be willing to share the theory or even better what was the remedy behind this issue. The fixative is 10% Neutral Buffered Formaliln and the cells in question that are "dropping out" which is what the pathologist is describing are melanocytes, especially with Sox-10 and Mart 1 antibodies. Thanks for the assistance, I definitely appreciate it very much. Best wishes, [image001] Debbie Siena, HT(ASCP)QIHC Empowering Anatomic Pathology Technical Support Manager, StatLab 2090 Commerce| McKinney, TX 75069 t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369 dsi...@statlab.com<mailto:dsi...@statlab.com>|www.statlab.com<http://www.statlab.com/> StatLab is an ISO 13485 Certified Company _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
