[Histonet] job opening
Hello Histonet, There is a job opening for a lab manager position at the University of Georgia. This is a small research lab in the College of Veterinary Medicine. There would be 2 techs, including yourself, and 2 pathologists. Since this is a state government job, the salary does not compete with private industry. However, there are many benefits to working at a state government post, including TRS Teachers Retirement (pension- based retirement) and free tuition at any Georgia State college, including UGA. If interested, apply here: https://www.ugajobsearch.com/postings/59337 Best, Lauren ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FISH question
Thanks for your advice. I use adhesive slides from Leica. Years ago we had Superfrosts of another brand (maybe Thermo) and I can't remember similar issues. Can you recommend a special brand for FISH? Have you ever tried to do FISH on a slide without adhesion? Gudrun -Ursprüngliche Nachricht- Von: Whitaker, Bonnie [mailto:bonnie.whita...@osumc.edu] Gesendet: Dienstag, 19. Februar 2019 18:54 An: 'Mark Tarango'; Gudrun Lang Betreff: RE: [Histonet] FISH question I know a few years ago, we ran into the same issue, and the problem actually was with the slides. We were using cheaper slides for most of histology at the time, but had to purchase "higher end" slides for the FISH. Thanks, Bonnie Bonnie P. Whitaker AP Operations Director The Ohio State University Wexner Medical Center Department of Pathology N305 Doan Hall 410 West 10th Avenue Columbus, Ohio 43210 614.293.8418 FAX 614.293.2779 Pager: 614.293.7243 ext. 5013 -Original Message- From: Mark Tarango via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, February 19, 2019 12:43 PM To: Gudrun Lang Cc: HistoNet Subject: Re: [Histonet] FISH question Hi Gudrun, Are you sure you have digested long enough with pepsin? If the tissue is not well digested you will see background. We use sodium thiocyanate for pretreatment reagent, not citric buffer. These are my first thoughts. Mark On Tue, Feb 19, 2019 at 9:18 AM Gudrun Lang via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Dear histonetters! > > I have difficulties with my FISH preparation on FFPET. I struggle with > massive background. It looks like a thick fluorescent film. > > The signals can't be seen because of the background. Even the nuclei are > hard to see. > > The background is within the tissue but also surrounds it. Therefore it > must > be directly on the glass slide. > > > > The slide is clear after deparaffination and after pretreatment with citric > buffer and pepsin. After the pepsin the slides are rinsed in 2xSSC, then > 50%-70%-96%-100% ethanol (p.a.). > > And then the slides are airdried. > > On the dry slides foggy streams appear. The slides become turbid. When I > rinse them again in graded ethanols it becomes better but still a little > turbid. > > After hybridisation and stringent washing the slides are air-dried again > and > coverslipped with Dapi. > > When looking at the slides in the fluorescence microscope the trouble > arises. > > > > My assumption is, that there is a remnant of the salt of the SSC buffer. > How > can I inhibit this deposit? Can I replace the buffer with water without any > harm to the tissue? > > Or ist there a different cause for the turbidiy? > > I use fresh reagenses from xylene to buffer and ethanol. > > Any hints are welcome. > > > > Thanks in advance > > Gudrun Lang > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu _mailman_listinfo_histonet=DwICAg=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFE kBfs4=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY=gzHjMQcM0W22Saad2E8Pgv a_UOfvxrD1xD0IQ57lDUY=rVkT1YjvFe8uXhvIGPusCI6h-qfQm2I-v86i1XIRePc= > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu _mailman_listinfo_histonet=DwICAg=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFE kBfs4=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY=gzHjMQcM0W22Saad2E8Pgv a_UOfvxrD1xD0IQ57lDUY=rVkT1YjvFe8uXhvIGPusCI6h-qfQm2I-v86i1XIRePc= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FISH question
Hi Gudrun, Are you sure you have digested long enough with pepsin? If the tissue is not well digested you will see background. We use sodium thiocyanate for pretreatment reagent, not citric buffer. These are my first thoughts. Mark On Tue, Feb 19, 2019 at 9:18 AM Gudrun Lang via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Dear histonetters! > > I have difficulties with my FISH preparation on FFPET. I struggle with > massive background. It looks like a thick fluorescent film. > > The signals can't be seen because of the background. Even the nuclei are > hard to see. > > The background is within the tissue but also surrounds it. Therefore it > must > be directly on the glass slide. > > > > The slide is clear after deparaffination and after pretreatment with citric > buffer and pepsin. After the pepsin the slides are rinsed in 2xSSC, then > 50%-70%-96%-100% ethanol (p.a.). > > And then the slides are airdried. > > On the dry slides foggy streams appear. The slides become turbid. When I > rinse them again in graded ethanols it becomes better but still a little > turbid. > > After hybridisation and stringent washing the slides are air-dried again > and > coverslipped with Dapi. > > When looking at the slides in the fluorescence microscope the trouble > arises. > > > > My assumption is, that there is a remnant of the salt of the SSC buffer. > How > can I inhibit this deposit? Can I replace the buffer with water without any > harm to the tissue? > > Or ist there a different cause for the turbidiy? > > I use fresh reagenses from xylene to buffer and ethanol. > > Any hints are welcome. > > > > Thanks in advance > > Gudrun Lang > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipper
Happy Thursday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 just for $10K (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the flexibility of the Coverslipper it is easy and straightforward to operate and cleaning and maintenance is simple to do. It is small enough to fit into fume cabinets, easy to move around and accepts a variety of commercial mounting media. Will take any offer, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Best Interview Question EVER!! How would YOU answer it?
Dear Histonetters, I hope you are having a great week! Coming soon to an interview near you Have you seen Facebooks Million Dollar interview question? Here it is On your very best day at work - the day you come home and think you have the best job in the world -what did you do that day? Facebook uses this question to find out what their candidates are passionate about. If you were asked this question how would you answer it? If your answer doesnt match up with a day At your current position We NEED to talk! I can help you find the position that you are passionate about, the place where every day is your best day at work. I have many histotechs that I have worked with over the years that can answer that question with the enthusiasm it deserves because I helped them find that job. Let me help you too! Histonetters, If you are looking for a job today, tomorrow, in 3 months, 6 months, a year, 5 years. I am here for you. My best day at work is every day! Since my passion is to find the right opportunity for you! Here is a list of my current opportunities: VASenior Histology Tech VAHistotechnician ALDermpath Histotech OH Territory Sales Manager MA Dermpath Histotech MA Histotechnician (32 hours) MD Histotechnician CA Grossing Histotech TN Grossing Histotech NY Histotechnician (NY lic. Req.) All of these opportunities are full time permanent positions with some of the leading employers nationwide. There are 1st, 2nd and 3rd shift positions open. Experienced and entry level and ASCP or eligible!! My clients offer excellent compensation, benefits and relocation/sign on bonuses. And they cant wait to speak to you!!! Please contact me at rel...@earthlink.net or toll free at 866-607-3542 or call/text me on my cell at 407-353-5070 if you are interested in more information on any of these positions or if you would like for me to work on a custom job search for you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! #jobs4myhistopeeps Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FISH question
Dear histonetters! I have difficulties with my FISH preparation on FFPET. I struggle with massive background. It looks like a thick fluorescent film. The signals can't be seen because of the background. Even the nuclei are hard to see. The background is within the tissue but also surrounds it. Therefore it must be directly on the glass slide. The slide is clear after deparaffination and after pretreatment with citric buffer and pepsin. After the pepsin the slides are rinsed in 2xSSC, then 50%-70%-96%-100% ethanol (p.a.). And then the slides are airdried. On the dry slides foggy streams appear. The slides become turbid. When I rinse them again in graded ethanols it becomes better but still a little turbid. After hybridisation and stringent washing the slides are air-dried again and coverslipped with Dapi. When looking at the slides in the fluorescence microscope the trouble arises. My assumption is, that there is a remnant of the salt of the SSC buffer. How can I inhibit this deposit? Can I replace the buffer with water without any harm to the tissue? Or ist there a different cause for the turbidiy? I use fresh reagenses from xylene to buffer and ethanol. Any hints are welcome. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] comparison between tissue processors
Hello everybody, we are buying a new tissue processor for the lab and, at the moment, we are considering these three models: Thermo Excelsior AS Leica HistoCore PEARL SLL MTM I/II I would very much appreciate your thoughts/experience regarding any of these models. We are a small research lab, so, normally, no huge burden of blocks. Thanks a lot for your help Regards Julio Benavides Silván Instituto de Ganadería de Montaña (CSIC-Universidad de León) 24346. Grulleros, León +34 987317156 ext.861162 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
