Re: [Histonet] Muscle Enzyme Histochemistry
Hi, I presume you are talking about light microscopy. Glutaraldehyde is often used for electron microscopy. If that is the case, ignore this. Glutataraldehyde fixes similarly to formalin, so the morphology should not be much different. However, it leaves the tissue with free aldehyde groups and these can react with Schiff's reagent or silver reductions. That means if doing a PAS or methenamine silver you should first (before oxidation) do an aldehyde block. I recommend aniline-acetic acid for 30 minutes, although others work as well. If you don't you will have a dark background. It is unlikely that enzyme histochemistry will be successful, but you never know. Esterase may still be demonstrable, possibly, but the ATPase iso-enzymes are unlikely to survive. Go to http://stainsfile.info and read the pages on glutaraldehyde for more information about it. Bryan Llewellyn MONICA D. LOCKHART via Histonet wrote: Hello Histology World!!! Can muscle placed in Gluteraldehyde be processed for structure? In this situation, all tissue was placed in Glut and we need to perform Enzyme Histochemistry but we have no fresh tissue. Thanks for your help. Monica D. Lockhart, BBA, HT (ASCP) PBT Supervisor Clinical Labs Histology Loyola University Medical Center 2160 S. First Ave, Bldg 110 Rm 2290 Maywood, IL 60153 (o) 708.327.2608 (c) 708.692.8361 monica.lockh...@luhs.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TISSUE PUNCH FOR MAKING CONTROL BLOCKS
Hi All, Could some one give a good tissue punch product for making control blocks for IHC? Thanks Nirmala Srishan Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica bond Max
Hi all, Is there anyone interesting to the Leica bond max IHC stainer? Thanks, Kai Hong Research histotechnologist kh...@waxitinc.com 202 - 2386 East Mall Vancouver, B.C. V6T 1Z3 Canada Office: 604-822-1595 Lab: 604-822-2589 Fax: 604-822-0660 www.waxitinc.com -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Monday, February 25, 2019 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 183, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Muscle Enzyme Histochemistry (MONICA D. LOCKHART) -- Message: 1 Date: Mon, 25 Feb 2019 17:33:33 + From: "MONICA D. LOCKHART" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Muscle Enzyme Histochemistry Message-ID: <5ab0d33096f44da1bd81ad68d5be3...@luhs.org> Content-Type: text/plain; charset="us-ascii" Hello Histology World!!! Can muscle placed in Gluteraldehyde be processed for structure? In this situation, all tissue was placed in Glut and we need to perform Enzyme Histochemistry but we have no fresh tissue. Thanks for your help. Monica D. Lockhart, BBA, HT (ASCP) PBT Supervisor Clinical Labs Histology Loyola University Medical Center 2160 S. First Ave, Bldg 110 Rm 2290 Maywood, IL 60153 (o) 708.327.2608 (c) 708.692.8361 monica.lockh...@luhs.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- End of Histonet Digest, Vol 183, Issue 20 * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Muscle Enzyme Histochemistry
Hello Histology World!!! Can muscle placed in Gluteraldehyde be processed for structure? In this situation, all tissue was placed in Glut and we need to perform Enzyme Histochemistry but we have no fresh tissue. Thanks for your help. Monica D. Lockhart, BBA, HT (ASCP) PBT Supervisor Clinical Labs Histology Loyola University Medical Center 2160 S. First Ave, Bldg 110 Rm 2290 Maywood, IL 60153 (o) 708.327.2608 (c) 708.692.8361 monica.lockh...@luhs.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
