Re: [Histonet] Stainer vs. Stainer

[Gudrun Lang via Histonet]

Hi Terri,
Have you actually used the Spectra from Leica? This is the follower of
ST5020 and CV5020.
I am also interested in some feedback on this instrument. 

Gudrun Lang

-Ursprüngliche Nachricht-
Von: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Donnerstag, 14. März 2019 20:10
An: 'histonet@lists.utsouthwestern.edu'
Betreff: [Histonet] Stainer vs. Stainer

Hi Alison - 
I've used both stainers and like both of them a lot.  Both were super
reliable and easy to use.  However, coverslipping is a different story.
I've used both film and glass.  About film - super quick, super easy, but -
the purity of the xylene used to coverslip from film must be absolute.
Anyone who has experienced film pulling off the slides in storage had a
miniscule portion of water carried down the acohols and into the xylene. If
it were glass, the process is a bit more forgiving of water contaminent. The
absolute alcohols leading to the end xylenes must be kept very fresh.  I
kept film slides for over 20 years, no problem.  If you are looking into
digital pathology, I would check with vendors to see if film is acceptable.
I don't know.
As to coverslippers, we've been using the Sakura glass now for 10 years and
love it.  I can't compare it to the newer Leica Glass, but 10 years ago my
techs all preferred the Sakura because it had fewer moving parts and the
maintenance was easier.  I hope this helps.  Good Luck, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

   6. stainer v. stainer (Perl , Alison)
   
Message: 6
Date: Wed, 13 Mar 2019 20:08:14 +
From: "Perl , Alison" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] stainer v. stainer


Hi all
We are getting ready to purchase a new H&E stainer/coverslipper, and are
considering the Sakura Prisma Plus (tape) and the Leica Spectra (glass).
Does anyone have good or bad feedback on either instrument, and/or tape v.
glass? We've always had glass, but of course the coverslippers need more
maintenance, take longer to dry, more expensive than tape, etc etc. So we
are very interested in tape, but still a little hesitant about the old
problems of yellowing and peeling after 10+ years. Since we're in NY, we
have to keep all slides for 20 years

Any thoughts are appreciated!

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
CareMount Medical
110 South Bedford Rd
Mount Kisco, NY 10549
(914) 302-8424
ap...@cmmedical.com
www.caremountmedical.com



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[Histonet] tape for digital scanning...RE: Stainer vs. Stainer

[Morken, Timothy via Histonet]

I'll note that we have used glass coverslips in histology forever but are 
switching to tape not for the speed (though that is appreciated) but because 
the tape dries instantly and can be put in a slide scanner right away, while 
glass slides cannot until dry enough - takes a lot longer. In fact, we were 
cutting tape strips down to use as coverslips for FS slides so we could scan 
right away for remote Dx while the OR was waiting. 

The taped slides scan fine and the images are fine. 

The primary complaint about taped slides is that the tape scratches and so 
makes microscopy a bit more difficult, and we pull slides constantly for 
various conferences. However, with scanned images it makes no difference. The 
slides are scanned when the tape is pristine, stored and rarely pulled again. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, March 14, 2019 12:10 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Stainer vs. Stainer

Hi Alison - 
I've used both stainers and like both of them a lot.  Both were super reliable 
and easy to use.  However, coverslipping is a different story.
I've used both film and glass.  About film - super quick, super easy, but - the 
purity of the xylene used to coverslip from film must be absolute. Anyone who 
has experienced film pulling off the slides in storage had a miniscule portion 
of water carried down the acohols and into the xylene. If it were glass, the 
process is a bit more forgiving of water contaminent. The absolute alcohols 
leading to the end xylenes must be kept very fresh.  I kept film slides for 
over 20 years, no problem.  If you are looking into digital pathology, I would 
check with vendors to see if film is acceptable.  I don't know.
As to coverslippers, we've been using the Sakura glass now for 10 years and 
love it.  I can't compare it to the newer Leica Glass, but 10 years ago my 
techs all preferred the Sakura because it had fewer moving parts and the 
maintenance was easier.  I hope this helps.  Good Luck, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

   6. stainer v. stainer (Perl , Alison)
   
Message: 6
Date: Wed, 13 Mar 2019 20:08:14 +
From: "Perl , Alison" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] stainer v. stainer


Hi all
We are getting ready to purchase a new H&E stainer/coverslipper, and are 
considering the Sakura Prisma Plus (tape) and the Leica Spectra (glass). Does 
anyone have good or bad feedback on either instrument, and/or tape v. glass? 
We've always had glass, but of course the coverslippers need more maintenance, 
take longer to dry, more expensive than tape, etc etc. So we are very 
interested in tape, but still a little hesitant about the old problems of 
yellowing and peeling after 10+ years. Since we're in NY, we have to keep all 
slides for 20 years

Any thoughts are appreciated!

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
CareMount Medical
110 South Bedford Rd
Mount Kisco, NY 10549
(914) 302-8424
ap...@cmmedical.com
https://protect2.fireeye.com/url?k=0a167f0a-5656125c-0a165817-0cc47adb57f0-ef5d177b74bfb01b&u=http://www.caremountmedical.com/



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[Histonet] Stainer vs. Stainer

[Terri Braud via Histonet]

Hi Alison - 
I've used both stainers and like both of them a lot.  Both were super reliable 
and easy to use.  However, coverslipping is a different story.
I've used both film and glass.  About film - super quick, super easy, but - the 
purity of the xylene used to coverslip from film must be absolute. Anyone who 
has experienced film pulling off the slides in storage had a miniscule portion 
of water carried down the acohols and into the xylene. If it were glass, the 
process is a bit more forgiving of water contaminent. The absolute alcohols 
leading to the end xylenes must be kept very fresh.  I kept film slides for 
over 20 years, no problem.  If you are looking into digital pathology, I would 
check with vendors to see if film is acceptable.  I don't know.
As to coverslippers, we've been using the Sakura glass now for 10 years and 
love it.  I can't compare it to the newer Leica Glass, but 10 years ago my 
techs all preferred the Sakura because it had fewer moving parts and the 
maintenance was easier.  I hope this helps.  Good Luck, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

   6. stainer v. stainer (Perl , Alison)
   
Message: 6
Date: Wed, 13 Mar 2019 20:08:14 +
From: "Perl , Alison" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] stainer v. stainer


Hi all
We are getting ready to purchase a new H&E stainer/coverslipper, and are 
considering the Sakura Prisma Plus (tape) and the Leica Spectra (glass). Does 
anyone have good or bad feedback on either instrument, and/or tape v. glass? 
We've always had glass, but of course the coverslippers need more maintenance, 
take longer to dry, more expensive than tape, etc etc. So we are very 
interested in tape, but still a little hesitant about the old problems of 
yellowing and peeling after 10+ years. Since we're in NY, we have to keep all 
slides for 20 years

Any thoughts are appreciated!

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
CareMount Medical
110 South Bedford Rd
Mount Kisco, NY 10549
(914) 302-8424
ap...@cmmedical.com
www.caremountmedical.com



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Re: [Histonet] ER/PR Control

[Terri Braud via Histonet]

Hi Barbi - 
We are somewhere in-between with our ER/PR Controls.  When performing lot to 
lot validations, we use our in-house control, which only has a 0 and a 3+, BUT 
we also use a commercially prepared control that contains 4 cores, 0. 1+, 2+, 
3+.  Once the new lot of antibody has been validated against both controls, 
then we only use our inhouse control with the 0 and 3+.  It just saves on time 
and money.
Hope this helps, Sincerely, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

Today's Topics:
   2. ER/PR controls (Moe, Barbi A)
   3. Re: ER/PR controls (Colleen Forster)
Message: 2
Date: Wed, 13 Mar 2019 17:43:51 +
From: "Moe, Barbi A" 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] ER/PR controls
For anyone making in-house ER/PR controls could you comment on what tissue you 
have in your control block -- one piece of positive tumor regardless of 
reactivity strength 1+, 2+, 3+ (weak, medium, strong) or multiple pieces to 
represent each level of reactivity?

Also, does anyone order commercially their ER/PR controls to achieve this level 
of representation in their control block?
Any thoughts are appreciated - thank you.

Barb Moe
Gundersen Health System
La Crosse WI
ba...@gundersenhealth.org


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[Histonet] Artifact IHC

[Bliven, Laura M via Histonet]

I have seen an artifact on an Estrogen IHC slide, although rare, our 
pathologists have also seen it on outreach slides.
It appears somewhat granular and/or dot-like and it is not on the Progesterone 
slide.
The Estrogen uses a high pH and our Progesterone does not. I assume it's caused 
by the high pH epitope retrieval, but would like a reference to this theory.
Any ideas?

Laura
bliven.la...@marshfieldclinic.org

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Re: [Histonet] P16

[Michael Kent via Histonet]

You might check Cancer Diagnostics, inc..  Their rep told us they have an IVD 
p16.

Best,
Mike
 
-Original Message-
From: Charles Riley via Histonet  
Sent: Wednesday, March 13, 2019 1:24 PM
To: Bernice Frederick 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] P16

We by predilute from BioGenix

On Wed, Mar 13, 2019 at 12:27 PM Bernice Frederick via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Who is the best and current supplier for p16? We were using 
> biocare,but evidently it is no longer available from them. And no 
> Santa Cruz per my IHC tech.
> Thanks,
> Bernice
>
> Bernice Frederick
> Pathology Core Facility
> Robert H. Lurie Cancer Center
> 710 North Fairbanks Court
> Olson 8-421
> 312-503-3723
> b-freder...@northwestern.edu
>
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>


-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] Surgical Hardware

[Heckford, Karen - SMMC-SF via Histonet]

Good Morning,
We have an abundance of surgical hardware we need to get rid of from our 
Pathology Department.   How do you get rid of your surgical hardware?


Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

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