[Histonet] Microtome Accident Prevention Ideas
Good Afternoon Histonetters, Three weeks ago, I was cutting at a manual microtome and when I finished laying my ribbon on the waterbath and turned back to my block, I happened to nick my finger on the end of my microtome blade. It was a freak accident of my finger being in the wrong place at the wrong time. I required 2 stitches. No big deal. Histotechs often have scars. In fact this is my second one on this finger. The last incident was over 10 years ago at another institution. I was invited today to a meeting of some higher level management to discuss the incident and to come up with interventions to prevent these incidents in the future. This was a freak accident that couldn't have been prevented, in my opinion. It is impossible to transfer a ribbon to your waterbath and put the blade guard up at the same time. I do want to come to the meeting with ideas though because I am walking into a room full of people who have never laid eyes on a microtome and I don't want them to come up with ideas for me. Here are some measures that we already have in place: 1) We always lock our wheel when we are not turning it. 2) We put our blade guard up when we are changing out a block or when we walk away from our microtome. 3) After cleaning our microtomes, we put them back together and check to make sure they are locked appropriately. 4) We never leave a blade in the microtome when we are leaving for the day. Thanks for your help, Kelly Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) Histology Supervisor-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pai...@nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Softening Calf Hooves
Sandra: We here at the Dermatopathology department of UW Mad have found: -10% Potassium Hydroxide works best for us on our nails. -We usually only need to have it in solution for a few hours. As you are trying to soften hoof, I might play with both concentration and time in solution. - I don't believe Monty Python ever actually stated the relative air speed of either type of unladen swallow. Say HI to Vicki K for me. Claire Ingles From: Rene J Buesa via Histonet Sent: Thursday, March 21, 2019 12:32 PM To: Sandra Cheasty; Histonet (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Softening Calf Hooves WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Either potassium or sodium hydroxide will work at 10% aq. solution. Check it daily until soft enough.As to your "swallow" question, I "Goggled" your question and the answer is the following" In the end, it's concluded that the airspeed velocity of a (European) unladen swallow is about 24 miles per hour or 11 meters per second. But, the real question is not about swallows at all. King Arthur in the movie had two coconut shells that he banged together to simulate the sound of a horse galloping.Jul 7, 2013 René On Thursday, March 21, 2019 12:06 PM, Sandra Cheasty via Histonet wrote: Hello all, I have 1 inch thick cross sections (band saw) of calf hooves. The pathologist has already decaled the bone, and now we have to soften the hooves so she can take thinner sections for processing. *Which is better, potassium hydroxide or sodium hydroxide, and what concentration? *How often should they be checked for softening? *What is the relative air speed of an un-laden swallow? (European) Thanks for your help! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Looking for vendor for Zeus Wash solution
You can also use Michel's buffer. Electron Microscopy Sciences has it. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com On Thursday, March 21, 2019, 12:29:45 PM CDT, Martha Ward-Pathology via Histonet wrote: I am having trouble locating a distributor for the Zeus Wash solution (cat#103) that we use to wash our skin and renal biopsies. Cardinal and Fisher do not seem to carry it and we do not have Zeus Scientific in our system as a vendor we can use. Any help would be appreciated! Thanks, Martha Ward Wake Forest Baptist Health Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Softening Calf Hooves
Either potassium or sodium hydroxide will work at 10% aq. solution. Check it daily until soft enough.As to your "swallow" question, I "Goggled" your question and the answer is the following" In the end, it's concluded that the airspeed velocity of a (European) unladen swallow is about 24 miles per hour or 11 meters per second. But, the real question is not about swallows at all. King Arthur in the movie had two coconut shells that he banged together to simulate the sound of a horse galloping.Jul 7, 2013 René On Thursday, March 21, 2019 12:06 PM, Sandra Cheasty via Histonet wrote: Hello all, I have 1 inch thick cross sections (band saw) of calf hooves. The pathologist has already decaled the bone, and now we have to soften the hooves so she can take thinner sections for processing. * Which is better, potassium hydroxide or sodium hydroxide, and what concentration? * How often should they be checked for softening? * What is the relative air speed of an un-laden swallow? (European) Thanks for your help! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for vendor for Zeus Wash solution
I am having trouble locating a distributor for the Zeus Wash solution (cat#103) that we use to wash our skin and renal biopsies.Cardinal and Fisher do not seem to carry it and we do not have Zeus Scientific in our system as a vendor we can use. Any help would be appreciated! Thanks, Martha Ward Wake Forest Baptist Health Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Softening Calf Hooves
Hello all, I have 1 inch thick cross sections (band saw) of calf hooves. The pathologist has already decaled the bone, and now we have to soften the hooves so she can take thinner sections for processing. * Which is better, potassium hydroxide or sodium hydroxide, and what concentration? * How often should they be checked for softening? * What is the relative air speed of an un-laden swallow? (European) Thanks for your help! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ER/PR/Her2 Controls
Pantomics has several expression controls and has high quality products. https://www.pantomics.com/tissue-arrays/breast Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: John Garratt via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, March 20, 2019 1:50 PM To: Dessoye, Michael Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ER/PR/Her2 Controls I suggest you contact Mark Rees at StatLab. mr...@statlab.com John https://protect2.fireeye.com/url?k=6fdad446-339ab910-6fdaf35b-0cc47adb57f0-5caa0b3d866cb5e0=http://www.ciqc.ca/ ‐‐‐ Original Message ‐‐‐ On Wednesday, March 20, 2019 12:42 PM, Dessoye, Michael via Histonet wrote: > Hello Histonet, > > Can anyone recommend a commercial source of ER/PR/Her2 control tissue (array > blocks would be preferred)? I'm looking for something similar to the breast > analyte control from Cell Marque, which is RUO: > > https://protect2.fireeye.com/url?k=4d82c7f4-11c2aaa2-4d82e0e9-0cc47adb57f0-946655d0b2e511d4=http://www.cellmarque.com/cms/histocyte/breast-DR.php > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor > | Commonwealth Health Laboratory Services | > mjdessoye@commonwealthhealth.netmailto:mjdess...@commonwealthhealth.net | 575 > N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: > 570-552-1484 > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is intended > only for the use of the individual(s) and entity named in the message. If you > are not an intended recipient of this message, please notify the sender > immediately and delete the material from your computer. Do not deliver, > distribute or copy this message and do not disclose its contents or take any > action in reliance on the information it contains. > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
