Re: [Histonet] Victoria blue for lung tissue

[Tony Henwood (SCHN) via Histonet]

Victoria Blue is the dye used in Miller's Stain for Elastic Tissue.
It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue:

Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148–149

Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical 
stains to identify pulmonary small vasculature, Journal of Histotechnology, 
40:3, 73-78

Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" 
Histologic 32(1): 12

Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 
23: 37-42.


Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


From: Bob Richmond via Histonet 
Sent: Saturday, July 27, 2019 4:10 AM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Victoria blue for lung tissue

Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE
lung tissue to exam thickness of artery. Could anybody recommend a good
vendor of this reagent kit?<<

You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and
several others. I couldn't find anyone who offers a kit, only the dry dye.
There are other dyes called Victoria Blue, reported to give the same
results.

You'd have to find a method for preparing it as an elastic stain, and I
couldn't find such a method either with Google or in my old books. The
requester needs to supply you with a method. I suspect the requester is
reading an old article. There are stains for elastic tissue (which is I
suppose what you want to "exam thickness of artery") that are a lot easier
to get.

Bob Richmond
Samurai Pathologist
Maryville TN
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Re: [Histonet] Victoria blue for lung tissue

[Bob Richmond via Histonet]

Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE
lung tissue to exam thickness of artery. Could anybody recommend a good
vendor of this reagent kit?<<

You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and
several others. I couldn't find anyone who offers a kit, only the dry dye.
There are other dyes called Victoria Blue, reported to give the same
results.

You'd have to find a method for preparing it as an elastic stain, and I
couldn't find such a method either with Google or in my old books. The
requester needs to supply you with a method. I suspect the requester is
reading an old article. There are stains for elastic tissue (which is I
suppose what you want to "exam thickness of artery") that are a lot easier
to get.

Bob Richmond
Samurai Pathologist
Maryville TN
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[Histonet] TGIF Histopeeps! Run your own lab in Columbus, GA

[Pam Barker via Histonet]

Hello Histopeeps,
TGIF!
I hope you are looking forward to a fantastic weekend.
I have a new position to tell you about near Columbus, GA.
This Is An Opportunity To Run Your Own Derm Histology Lab!! 
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I can be reached toll free at the office at 866-607-3542 or
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Pam Barker
President/Senior Recruiting Specialist-Histology
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Re: [Histonet] Major skin resections - CPT coding

[Joe W. Walker, Jr. via Histonet]

Hi Dr. Cartun,

We have had similar discussions about this in our institution, too.  
Unfortunately, according to our contracted lab compliance person, the correct 
code for all skin lesions is 88305.

Below is an excerpt from Dennis Padget's coding manual (now owned by American 
Pathology Foundation) that provides information related to this topic:
" Popular urban legend has it that it’s okay to report large or complex skin 
specimens—wide excisions, melanomas, and basal cell or Merkel cell with margins 
immediately come to mind—at the 88307 or 88309 charge level. Although these 
specimens unquestionably involve more work than lesser samples, what can’t be 
disputed is that they’re still very much accurately described by the label 
Skin, other than cyst/tag/debridement/plastic repair. Hence, you should ignore 
these myths. For added information on this important topic, refer to section 3 
of chapter 7.) Report code 88305 unconditionally for a skin specimen diagnosed 
as other than a plastic repair, debridement, cyst, or tag. You can’t change the 
code based on the clinical diagnosis, pathologic diagnosis (other than to rule 
out the 88302 and 88304 categories), number of blocks or slides, a 
complication, or an extenuating circumstance." (APF, 2016, p.245)

American Pathology Foundation. (2016). Pathology coding handbook (Ver. 16.3). 
Laguna Beach, CA: American Pathology Foundation.

Joe W. Walker, Jr. MS, SCT(ASCP)
Anatomical Pathology Manager
joewal...@rrmc.org, www.rrmc.org

-Original Message-
From: Cartun, Richard via Histonet 
Sent: Thursday, July 25, 2019 4:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Major skin resections - CPT coding

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I'm curious; how do you code major (complex) skin resections that also include 
soft tissue?  These cases often have 10-30 paraffin blocks and lots of H 
slides.  I'm thinking that they could be coded 88307 (simple resection) or 
88309 (complex resection).  I found out that our Dermatopathologist was coding 
these as "88305" and I don't think that is appropriate.  Thank you in advance 
for your comments.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


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