[Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure

[Donovan, Mark via Histonet]

Sabash,

What I think you may be referring to is the use of a surfactant in the antigen 
retrieval solution to lower the surface tension. The theory being it reduces 
bubble formation in the retrieval solution at high temperature and hence 
disruption of tissue.  Before moving to commercial antigen retrieval solutions 
we would add Tween 20 to a concentration of 0.05% to our in house citrate or 
EDTA retrieval solutions to achieve this effect.

Regards,
Mark


Original message:

Date: Wed, 16 Oct 2019 13:43:28 -0400
From: Maria Cruz mailto:mcruz8...@gmail.com>>
To: 
"histonet@lists.utsouthwestern.edu"

mailto:histonet@lists.utsouthwestern.edu>>
Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for
immunohistochemistry procedure
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mailto:capg7ww1f0dsbw85m8wnrz2pamxpgkqpxw0v9kx-svxmwbv1...@mail.gmail.com>>
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Subash:
Since the main purpose of using a pressure cooker for HIER is the rapid and 
reliable pre-treatment of sections while PREVENTING boiling and potential 
tissue detachment, you really shouldn?t be experiencing any bubbling. If you 
are, then  your PC isn?t sealed adequately  and the only way to fix that 
problem is to replace the gasket or find another way to tighten the lid..  I?ve 
never heard of anything that  you can put inside of a PC that can change the 
way it pressurizes,  so I?ll be following this thread to see if anyone else can 
contribute.

Maria

**

Hi there all

Can anybody please tell me if they have heard of or  perhaps know of a 
substance that can be placed in the pressure cooker during the antigen 
retrieval process for immunohistochemistry.  This prevents excessive bubbling 
and lifting up of sections.  I did somehow come across such info a while back, 
but seem to have lost it.  Many thanks.

Best Regards
Subash Govender






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[Histonet] RELIA HOT JOB ALERT! IHC Specialist for New England area. Contact me for info ASAP!

[Pam Barker via Histonet]

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[Histonet] ICYMI here is a link to part 3 of 3 in my series on Relocation - Relocation an Action Plan. And our latest histology opportunties on and off the bench!

[Pam Barker via Histonet]

Hi Histonetters,
ICYMI - In case you missed it!!
I am so excited!!  My latest article has been published in the NSH Quarterly
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Make the Cut
 My latest article is entitled:  Relocation – An Action Plan   
This is part 3 of 3 in my series on relocation.  You can find a link to
parts 1&2 in this article as well!
This is the link to my article on the NSH’s Fixation on Histology Blog and
it is also in the current edition of the NSH Career Newsletter.

Here is the link to the article:
https://www.fixationonhistology.com/post/relocation-part-3-action-plan

If you have a minute to read it I would love to hear what you think.
If you can’t get to the article with this link let me know and I will send
you a copy of it. 
Histopeeps, we also have amazing opportunities nationwide!
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4. Nashville   Histology Supervisor
5. Kalamazoo   Applications Specialist
6. Orlando Histology Instructor

HT or HTL or elig opportunities:
1. California  Northern CA IHC Specialist
2. California  Northern CA Histology tech
3. N. CarolinaRaleigh/Durham area Histology Tech
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If You or Anyone You Know Might Be Interested In a New Opportunity, Please
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If you want some additional information or to set up a time to chat please
call me toll free at 866-607-3542 or email me at rel...@earthlink.net 

Have a great day.  I look forward to hearing back from you.

 Thanks-Pam

#jobs4myhistopeeps 
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
https://www.facebook.com/RELIASolutionsforhistologyprofessionals
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





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Re: [Histonet] cleaning old charged slides

[Dave Juliano via Histonet]

Hi Atsoka! I’d be willing to trade you fresh slides for those foggy ones-
studying effects of age on charges slides.  Contact me at: Dave.c.juliano
at gmail dot com if you’re interested!

On Thu, Oct 17, 2019 at 8:24 AM Atoska Gentry via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello,
>
> Has anyone had success cleaning old charged slides which have become foggy
> over time.
>
> Thanks!
> ~Atoska
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[Histonet] procuring fresh tumor tissue

[Curt Tague via Histonet]

Hello again histonet-ers-

I am working with a company do validate a new rapid (another new) tissue 
processor... we are currently in the process of submitting a proof of concept 
paper to CAP then will submit a formal white paper with  data. What I am 
curious about is locating tumor tissue that can be used to parallel this study, 
obviously one part processed with the standard process we are all accustomed to 
and the other piece processed with this new technology. We are running all the 
necessary tests to show consistency and comparable results, H, special 
stains, IHC, DNA and RNA extraction... what I could use a little help with is 
sourcing some tumor that has not been in formalin for weeks and weeks... 
specifically breast tumor and possibly lung tumor to demonstrate all the 
downstream tests are not compromised with this technology.
Is there any source out there someone could recommend and/or, if you are in a 
large hospital setting, is that something you might be able to assist with, 
when a tumor is identified and not entirely needed for diagnostics?

All the best to you all, thanks for your thoughts,

Curt




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Re: [Histonet] cleaning old charged slides

[Atoska Gentry via Histonet]

Hello,

Has anyone had success cleaning old charged slides which have become foggy over 
time.

Thanks!
~Atoska
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