Re: [Histonet] Cell block preparations

[Tony Henwood (SCHN) via Histonet]

Hi Jennifer,

I have had excellent success with lysing the red blood cells (using  Isotonic 
Ammonium Chloride) prior to cell block preparation with thromboplastin-plasma. 
The lysing solution contains EDTA so you will need to add a few drops of 1% 
calcium chloride. Method as follows:

Lysis solution
Ammonium Chloride4.5g
Potassium carbonate  0.5g
EDTA 0.0186g
Distilled water  500mls

Method:
1.  Centrifuge bloody fluid.
2.  Remove supernatant and add equal volume of lysis solution.
3.  Resuspend and incubate for 5 minutes at 4oC.
4.  Centrifuge, if blood still remains, then repeat from step 2.
5. Rinse in Hanks or RPMI, centrifuge.
6.  Mix pellet in a few drops of plasma.
7. Add thromboplastin and a few drops  of 1% Calcium Chloride, mix gently 
and allow clot to form.
8. Add 10% buffered formalin and fix and process as usual.

Reference:  
Kuenen-Boumiester etal (1996) Acta Cytolog 40:475-479

If you donot use the plasma clot method for cell block preparation, then use 
your preferred method after step 5.
The lysis solution can also be purchased commercially from several companies 
(eg Biolegend). It is commonly used for sample preparation for flow cytometry. 
Check the SDS to make sure it does not contain formaldehyde.


-Original Message-
From: Mac Donald, Jennifer via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, 17 January 2020 4:53 AM
To: Charles Riley ; Histo List 

Subject: Re: [Histonet] Cell block preparations

Acetic acid would work.

Get Outlook for iOS 
From: Charles Riley via Histonet 
Sent: Thursday, January 16, 2020 8:55:21 AM
To: Histo List 
Subject: [Histonet] Cell block preparations

  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.

What is the best way to remove excess blood from FNA sample collections before 
spinning them down into cell blocks?

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This message is intended for the addressee named and may contain confidential 
information. If you are not the intended recipient, please delete it and notify 
the sender.

Views expressed in this message are those of the individual sender, and are not 
necessarily the views of NSW Health or any of its entities.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Coverslipper

[Margaryan, Naira via Histonet]

Happy Thursday, 

We would like to sell our slightly used but in excellent condition Dako 
Coverslipper which was purchased in 2010 (purchase price was $ 25K). The 
instrument can handle up to 600 slides per hour making it one of the fastest on 
the market. In addition to the flexibility of the Coverslipper it is easy and 
straightforward to operate and cleaning and maintenance is simple to do. It is 
small enough to fit into fume cabinets, easy to move around and accepts a 
variety of commercial mounting media.

Picture by request.

Will take any offer,
Naira


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RVU for Histology

[Heckford, Karen - SMMC-SF via Histonet]

Good Morning,
I think we are getting the short end of the stick when it is coming to 
productivity.   Can anyone give me insight on how they are calculating their 
RVU's for productivity in the Histology Lab?   I am busy as all get out and 
administration is saying our productivity is low.

Thanks,

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
recipient(s) named above. If the reader of this message is not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you have received this document in error.  Any further review, dissemination, 
distribution, or copying of this message is strictly prohibited.  If you have 
received this communication in error, please notify us  immediately by reply 
email.  Thank you

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] needing IHC contol tissue

[LeRoy Brown via Histonet]

Would anyone happen to have a small pc of normal stomach they are willing to
part with for my IHC control for GAS6 stain?

Please let me know at 1...@comcast.net  

Thanks

LeRoy Brown HT(ASCP) HTL

 

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] H&E trouble shooting

[Bert Dotson via Histonet]

Excessive heat during slide drying can look like this but I think Liz has the 
most likely culprit. More specifically I would say there has been water 
carryover into your first clearing station during processing. Reagents not 
changed frequently enough or a low-grade mistakenly placed where 100% should 
be. Regards, Bert


-Original Message-
From: Liz Chlipala  
Sent: Wednesday, January 15, 2020 1:49 PM
To: Wang, Weixi 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] H&E trouble shooting

That an image of incomplete dehydration the samples have not been processed 
properly and adequately dehydrated.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: Wang, Weixi via Histonet 
Sent: Wednesday, January 15, 2020 11:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H&E trouble shooting

Hi,
I have some issues on the staining quality of biopsies. Please see the image 
link below, the overall staining looks good but the edge looks weird, no 
nuclear staining picked up. Under-fixed? Over-dehydrated in the processor? Or 
dried during grossing?
You expertise is greatly appreciated!

https://urldefense.proofpoint.com/v2/url?u=https-3A__imgur.com_gallery_kpLqRT9&d=DwIFAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=IkXb8WHm-SU8QcloSa5zh99VMreL429Dgfd2s0o3mrA&m=9Zt4pLGTo6dCvnjHleUlTkBYb8LQQc2G52vrJRkdg9M&s=8qzwuTTHy5TnitTfvT39e_57wxXJFJ2nygQXLWjNxVo&e=
 



Thank you,
Weixi




*** Important Notice About St. Josephs emails *** St. Josephs Regional Medical 
Center is using Zix to encrypt any e-mails containing Protected Health 
Information (PHI). If you receive an encrypted email from a person at St. 
Josephs the body of the message will indicate that you have a "New Zixcorp 
secure email from St. Josephs Regional Medical Center." You will need to click 
on the link in that email to retrieve the message. For more information please 
visit 
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.uapguide.com_st-2Djosephs-2Dhospital-2Dand-2Dregional-2Dmedical-2Dcenter_introduction&d=DwIFAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=IkXb8WHm-SU8QcloSa5zh99VMreL429Dgfd2s0o3mrA&m=9Zt4pLGTo6dCvnjHleUlTkBYb8LQQc2G52vrJRkdg9M&s=UzAs7b3GXocbvkjR-MIIe6DsHF6sRik5gbgS4bHc8X0&e=
 . For help in retrieving a secure email that you've received from St Josephs 
please go to 
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.uapguide.com_st-2Djosephs-2Dhospital-2Dand-2Dregional-2Dmedical-2Dcenter_receiving-2Dencrypted-2Demail&d=DwIFAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=IkXb8WHm-SU8QcloSa5zh99VMreL429Dgfd2s0o3mrA&m=9Zt4pLGTo6dCvnjHleUlTkBYb8LQQc2G52vrJRkdg9M&s=Gy141DOHMNtR-oJ6lFs8ERhL4hg4SJq3e8O2Cp-rV0M&e=
 .

CONFIDENTIAL COMMUNICATION
THIS TRANSMISSION IS INTENDED ONLY FOR THE INDIVIDUAL OR ENTITY TO WHICH IT IS 
ADDRESSED AND CONTAINS INFORMATION THAT IS CONFIDENTIAL. IF YOU HAVE RECEIVED 
THIS COMMUNICATION IN ERROR, PLEASE DELETE THE EMAIL AND CONTACT THE SENDER 
IMMEDIATELY. THIS INFORMATION MAY HAVE BEEN DISCLOSED TO YOU FROM CONFIDENTIAL 
RECORDS AND MAY BE PROTECTED BY FEDERAL AND STATE LAW. THIS INFORMATION MAY 
INCLUDE CONFIDENTIAL MENTAL HEALTH, SUBSTANCE ABUSE, ALCOHOL ABUSE AND/OR 
HIV-RELATED INFORMATION. FEDERAL AND STATE LAW PROHIBITS YOU FROM MAKING ANY 
FURTHER DISCLOSURE OF THIS INFORMATION WITHOUT THE SPECIFIC WRITTEN CONSENT OF 
THE PERSON TO WHOM IT PERTAINS, OR AS OTHERWISE PERMITTED BY LAW. ANY 
UNAUTHORIZED FURTHER DISCLOSURE IN VIOLATION OF THE LAW MAY RESULT IN A FINE OR 
JAIL SENTENCE OR BOTH. A GENERAL AUTHORIZATION FOR THE RELEASE OF THIS 
INFORMATION MAY NOT BE SUFFICIENT AUTHORIZATION FOR FURTHER DISCLOSURE.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIFAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc

Re: [Histonet] Cell block preparations

[Mac Donald, Jennifer via Histonet]

Acetic acid would work.

Get Outlook for iOS

From: Charles Riley via Histonet 
Sent: Thursday, January 16, 2020 8:55:21 AM
To: Histo List 
Subject: [Histonet] Cell block preparations

  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.

What is the best way to remove excess blood from FNA sample collections
before spinning them down into cell blocks?

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cell block preparations

[Charles Riley via Histonet]

What is the best way to remove excess blood from FNA sample collections
before spinning them down into cell blocks?

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histotech openings

[Katherine Marano via Histonet]

Hi histonetters~

I'm working with a top laboratory in a beautiful area of North Carolina
that is looking to hire histotechs. They currently have 2 positions open.
The hours are varied from 10 pm – 12 noon. They’ll either be 3rd shift or a
split 3rd/1st shift. Permanent positions with great benefits.

Let me know if you are interested in hearing more!

Sincerely,

Katherine Marano
*K.A. Recruiting, Inc.*
Your Partner in Healthcare Recruiting
10 Post Office Square, 8th Floor So.
Boston, MA 02109
P:  (617) 746-2750
F:  (617) 507-8009
kather...@ka-recruiting.com
http://www.ka-recruiting.com
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet