[Histonet] (no subject)
I'm all set to DYI my own phosphomolybdic/phosphotungstic acid solution for running a Masson Trichrome, but I see that the former reagent was purchased as "phosphomolybdic acid hydrate". All the recipes I have seen call for just "phosphomolybdic acid" but that is not a reagent that appears to exist without the water molecules coming along for the ride, unless you want to invest in chromatography grade stuff, which I think histology folks probably don't routinely do. The recipes all call for equal gram amounts of each of these crystals, so here's my question: Do I calculate how much weight the water is taking up and add more phosphomolybdic acid crystals (to account for its tagalong water molecules) than called for in the recipe? Or are these recipes already assuming that phosphomolybdic acid HYDRATE is the reagent you have on hand, and I should stick with equal amounts? This question is somewhat complicated by the fact that the molecular formula on the bottle is listed as H3Mo12O40P.XH2O ... which I think means the manufacturer won't bet on exactly how many water molecules are involved. Any advice appreciated! Katherine Davoli, BA, HTL(ASCP)CM Supervisor & Lab Manager, Tissue Culture & Histology Core Module Ophthalmic and Visual Sciences Research Center Department of Ophthalmology, University of Pittsburgh Mail Stop Code: EEI010901 930 Eye & Ear Institute, 203 Lothrop Street Pittsburgh, PA 15213 (412) 647-8256davol...@upmc.edu and kdav...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] alternative labels for zebra printer TLP 2742 connected to dako agilent autostainer plus
Hello, I purchase my solvent resistant labels and thermal ribbons for my Zebra printer from Electronic Imaging Materials. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Friday, January 24, 2020, 01:12:38 AM CST, richard.wild--- via Histonet wrote: Hi the list. Do you know alternativ labels for zebra printer TLP 2742, connected to dako agilent autostainer plus ? Did you try it ? It should have the same size, and same distance between labels, and be resistant to solvant and other chemicals, and thermal printed (instead of Agilent dako autostainer : S341730S3417Slide Label Kit, Large Flap, 6 rolls per kit, 3000 label) Best Richard ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
