Hi Curt I trust you and yours are well... Imho.....it will still be an excellent control, provided that you are processing it to Paraffin wax and use HIER ( antigen retrieval) before IHC/IF. Sure, as long as it has been optimally fixed ( immersion fixation with no agitation -BAD- leads to zonal variations of positivity) I have posted images on Histonet site of human tonsil that has remained in fix for a similar time before processing. Sure, I cannot guarantee all proteins will be retrievable but, in my XP, good fixation is the key....not the length of fixation.
Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
