Hi Jamie I do this often, for he same reason Ms and rat soft organs ( hollow and solid) leg bones, whole ankles, ears, cube of tissue containing cochlea, chick ribs..... Once the delay was 6 months. I allowed the cassettes with tissue in to solidify ( as do you) and then leave them in a labelled box to await embedding. Sometimes the researcher cannot embed at scheduled time and I would rather fix then process than have fixed tissues sitting in 70% alcohol until they can be processed and embedded on schedule ( sure, it doesn't adversely affect, in my xp, to leave in 70% alc at 4C provided the tissues have been optimally fixed ( meaning in 10% Formalin)
Obviously I strongly discourage any delay, telling them that it is deleterious Gotta keep them Researchers in order ;-) Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
