Re: [Histonet] JB4 embedded specimens

[Tony Henwood (SCHN) via Histonet]

I have found that that this gives a pleasing tinctorial result:

Haematoxylin and Eosin

Principle

Traditional HE procedures generally worked poorly on GM sections, with 
background staining of the plastic embedment and the loosening of sections from 
glass slides when alcoholic eosin is used. Aqueous solutions tend to stain 
poorly.

Sections are firstly stained with Celestine blue, followed by a regressive 
Harris's haematoxylin (excess stain is removed from the plastic with acid 
alcohol). The sections are then counterstained with buffered eosin Y/Phloxine B.

Solutions

1.   Celestine Blue - Iron Alum
2.   Harris's Haematoxylin
3.   Buffer for Eosin Y/Phloxine B.
  Solution A2.875ml Glacial Acetic acid in 500ml distilled water.
  Solution B4.1g Sodium Acetate in 500ml distilled water.

  150 ml Solution A + 350 ml Solution B. pH to 4.85.

4.   Eosin Y/Phloxine B.
  Eosin Y (CI 45380)5g.
  Phloxine B (CI 45410) 0.5g.
  Buffer500ml.
  Add 2 crystals Thymol.


Technique

1.  Hydrate sections.
2.  Stain in Celestine Blue 15 minutes.
3.  Wash in running water.
4.  Stain in Harris's Haematoxylin 15 minutes.
5.  Wash, differentiate and blue till surrounding plastic is clear.
6.  Stain in buffered Eosin for 6 minutes.
7.  Wash lightly in running water.
8.  Differentiate in alcohol till surrounding plastic is clear.
9.  Clear in Xylene and mount.


REFERENCES:

Castro,M.D., (1985) "A Haematoxylin - Eosin Phloxine Stain for tissues embedded 
in Glycol Methacrylate" J. Histotechn 8(1); 23-24.

Green,G.H.,Kurnein,F., (1981)  "Glycol Methacrylate embedding in General 
Histopathology " ACP Broadsheet 97.


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 



-Original Message-
From: Keena Leon Guerrero via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, 25 September 2020 8:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] JB4 embedded specimens

Hello All,

I was wondering if anyone has a good Hematoxylin and Eosin (H) staining 
protocol for JB4 embedded specimens?

Thanks!

Keena Leon Guerrero
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[Histonet] JB4 embedded specimens

[Keena Leon Guerrero via Histonet]

Hello All,

I was wondering if anyone has a good Hematoxylin and Eosin (H) staining
protocol for JB4 embedded specimens?

Thanks!

Keena Leon Guerrero
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[Histonet] QIHC Exam Preparers

[Jeanine Ronkowski via Histonet]

Hi Histo-peeps:I’m just wondering if someone can shed some light on who 
prepares the QIHC exam.  Everyone I’ve talked to about the test says that all 
of the questions are tricky and cover a lot older topics rather than more 
relavent information.  So how can we get some folks who understand today’s IHC 
world involved in this process?  TIA for your input
Sent from Yahoo Mail for iPhone
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Re: [Histonet] Fume hood filter

[John Garratt via Histonet]

You could purchase activated charcoal and replace the contents in the old filter

John

On Thu, Sep 24, 2020 at 8:57 AM, Hannen, Valerie via Histonet 
 wrote:

> Hi everyone.. hope all are well!!
>
> I have a slight dilemma... I have a "Fume-Gard" (old) fume hood and my 
> current vendor is out and will not be restocking. Is there anyone else out 
> there that is using this fume hood for coverslipping?? The dimensions are as 
> follows: H: 5.8 inches, D: 1.5 inches and W: 11.2.
>
> Can anyone steer me to a vendor who might have such filters with these 
> dimensions?
>
> Thanks !!
>
> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
> Section Chief, Histology
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville,Florida 32796
> T: (321)268-6333 ext. 7506
> F: (321) 268-6149
> valerie.han...@parrishmed.com
> www.parrishmed.com
>
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Re: [Histonet] Fume hood filter

[Shirley A. Powell via Histonet]

That website did not transfer right. 
www.pathologyserv.com
is the correct one

-Original Message-
From: Shirley A. Powell via Histonet  
Sent: Thursday, September 24, 2020 12:03 PM
To: Hannen, Valerie 
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Fume hood filter

Pathology Service, Inc, phone number is 1-866-398-9478 have these filters in 
different sizes.
https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.pathologyserv.com%2Fdata=02%7C01%7Cpowell_sa%40mercer.edu%7Ccb22de696fa24f4617fb08d860a3709c%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637365602375331091sdata=AfDrNObUZ7GOnLz4d%2FcdnCa9utZFs5sDHrZsEKla%2FI8%3Dreserved=0
 is their website.
Shirley Powell

-Original Message-
From: Hannen, Valerie via Histonet  
Sent: Thursday, September 24, 2020 11:57 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fume hood filter

Hi everyone.. hope all are well!!

I have a slight dilemma...  I have a "Fume-Gard" (old) fume hood and my current 
vendor is out and will not be restocking.  Is there anyone else out there that 
is using this fume hood for coverslipping??  The dimensions are as follows:  H: 
5.8 inches, D: 1.5 inches and W: 11.2.

Can anyone steer me to a vendor who might have such filters with these 
dimensions?

Thanks !!

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.parrishmed.com%2Fdata=02%7C01%7Cpowell_sa%40mercer.edu%7Ccb22de696fa24f4617fb08d860a3709c%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637365602375341086sdata=b1vZ9OKV0Uyeyuaa3hFLDmHGDxRWruFvyuYRHlsD1HY%3Dreserved=0

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Re: [Histonet] Fume hood filter

[Shirley A. Powell via Histonet]

Pathology Service, Inc, phone number is 1-866-398-9478 have these filters in 
different sizes.
www.PathologyServ.com is their website.
Shirley Powell

-Original Message-
From: Hannen, Valerie via Histonet  
Sent: Thursday, September 24, 2020 11:57 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fume hood filter

Hi everyone.. hope all are well!!

I have a slight dilemma...  I have a "Fume-Gard" (old) fume hood and my current 
vendor is out and will not be restocking.  Is there anyone else out there that 
is using this fume hood for coverslipping??  The dimensions are as follows:  H: 
5.8 inches, D: 1.5 inches and W: 11.2.

Can anyone steer me to a vendor who might have such filters with these 
dimensions?

Thanks !!

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.parrishmed.com%2Fdata=02%7C01%7Cpowell_sa%40mercer.edu%7C7a18833e0e3546e3818708d860a29cd9%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637365598820886967sdata=7Jb%2BCB8xVOh3U4VAbtweoVEOfhWlYI6MH7a9xL47nrs%3Dreserved=0

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[Histonet] Fume hood filter

[Hannen, Valerie via Histonet]

Hi everyone.. hope all are well!!

I have a slight dilemma...  I have a "Fume-Gard" (old) fume hood and my current 
vendor is out and will not be restocking.  Is there anyone else out there that 
is using this fume hood for coverslipping??  The dimensions are as follows:  H: 
5.8 inches, D: 1.5 inches and W: 11.2.

Can anyone steer me to a vendor who might have such filters with these 
dimensions?

Thanks !!

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com

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