Hi Kelly If the tubes that hold your frozen ab aliquots do not have a complete seal....the water in them will lyophilise, over time.
I have your problem occasionally, for that reason ( I have a couple of thousand frozen primary abs) Use snap-lid tubes ( I use 200microL PCR tubes) Hope that explains. Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
