Re: [Histonet] Detection systems for IHC's

2020-12-22 Thread Joe Myers via Histonet
Charles:
Since the literature for BioGenex’s detection reagents for their Xmatrx 
Infinity system (XvisTM) indicates use of an ‘enhancing’ reagent prior to 
application of the (tertiary) antibody/polymer/enzyme reagent, such a detection 
system would be defined as a ‘two-step’ method.  In contrast, Leica’s Refine 
reagent is a one-step reagent/method.  Generally speaking, two-step methods are 
usually more sensitive than one-step methods, but since I’ve not conducted a 
comparison of the results obtained with these quite different reagents/methods, 
I can’t offer any advice on which is actually ‘better’.  I hope you find this 
feedback useful.
Cheers,
Joe Myers, M.S., CT/QIHC(ASCP)



Message: 1
Date: Mon, 21 Dec 2020 18:57:57
From: Charles Riley 
To: "histonet@lists.utsouthwestern.edu"
   
Subject: [Histonet] Detection systems for IHC's
DOes anyone know what the comparison detection kit for the Xfinity Matrix from 
Biogenex would be in relation to the Bond Refine detection kits?

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[Histonet] Paraffin Preference

2020-12-22 Thread Bacon, Charles via Histonet
Hi Paula,

I have worked in a couple different labs and in one lab we used Paraplast Plus. 
Because of the DMSO, tissue infiltration is superb with that paraffin. 
Unfortunately DMSO is not good for stainless steel so when we had to replace 
our processor (now with a stainless retort) we also had to replace our 
paraffin. We opted for Polyscientific Infiltrating/Embedding Paraffin Prills, 
Catalog Number: c827. It is a bit more money but after testing several other 
paraffins including the Richard Alan ones, this was the closest we could get to 
the performance of the Paraplast Plus. This is only the case if you are using 
one paraffin for BOTH infiltration/processing and embedding. If so, this 
paraffin is the best for the money. If you want to have two separate paraffins, 
one for processing only and a second type for embedding then your options are 
wide open. You can opt for paraffin where the chemistry has less plastics for 
processing and more for embedding, giving you a more supportive block.  In the 
lab I am in now we also use the Polyscientific for both processing and 
embedding. 

Good luck,

Chuck Bacon, HTL(ASCP)CM 
Supervisor Histology
Baystate Medical Center
361 Whitney Ave., Holyoke, MA 01040
Telephone: 413-322-4786  Fax: 413-322-4790
charles.ba...@baystatehealth.org

-Original Message-
From: Paula  
Sent: Tuesday, December 22, 2020 9:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin Preference

Hi everyone..good morning.

 

I have samples of Fisher Scientific..or maybe it's Richard-Allan's paraffin.
It's called Histoplast PE.  

 

We use Leica's Paraplast Plus currently and I would like to know if anyone
can share their preference and if you can offer any comments about the
differences in the paraffin.  Also, can I mix the two?

 

Thanks so much in advance and have a great day.

 

Paula

Lab Manager

Bio-Path Medical Group



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[Histonet] Paraffin Preference

2020-12-22 Thread Paula via Histonet
Hi everyone..good morning.

 

I have samples of Fisher Scientific..or maybe it's Richard-Allan's paraffin.
It's called Histoplast PE.  

 

We use Leica's Paraplast Plus currently and I would like to know if anyone
can share their preference and if you can offer any comments about the
differences in the paraffin.  Also, can I mix the two?

 

Thanks so much in advance and have a great day.

 

Paula

Lab Manager

Bio-Path Medical Group

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