[Histonet] Antibody validation question
When doing an initial antibody validation CAP requires at least 90% concordance between the new test and the comparator test or expected values.Are there specified requirements for sensitivity, specificity and accuracy? Thanks in advance for any help provided! Martha Ward, Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 208, Issue 1
Have you considered any Digital Imaging platforms such as Roche's DP200 or Lieca's Aperio? Both of these systems provide superior imaging and you may even be able to build your own algorithms for immunofluourescent multiplexing. I am not a vendor but have seen both of these systems and evaluated them. The Roche DP 200 can scan the and give you X,Y,and Z imaging. You can literally focus up and down on the image to see layers. Just a thought. *John Frazier* *MT(ASCP), MBA* *Lean 6 Six Sigma Black Belt* *Healthcare Consultant* *C - 302-310-7567* *H - 704-847-0566* *wilfong1...@gmail.com * On Mon, Mar 1, 2021 at 1:00 PM wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > >1. Any comments on your usage of the following slide scanners? > (Katherine Bummer) > > > > -- Forwarded message -- > From: Katherine Bummer > To: "histonet@lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Mon, 1 Mar 2021 17:23:26 + > Subject: [Histonet] Any comments on your usage of the following slide > scanners? > Hello Histonetters, > > I am looking for some straight forward comments on a few slide scanners: > > * Zeiss Z1, > > * AkoyaBio Vectra Polaris and the > > * 3DHisTech P250FL. > > * > > I do almost exclusively immunofluourescent multiplexing, generally with > 4-5 targets and am looking at these scanners. I have had virtual demo's > but no live demos and am seeking EXCELLENT image quality as I will need to > be quantifying several targets within a sample. > > Just hoping if you are using one of these or have in the past that you can > give any good feedback on your experience with image quality. You can also > email me directly if you prefer either to my personal email below or to my > work email that is on this post. > > Thank you! > > > > Best regards, > > Katherine Bummer > bumkat0...@gmail.com > > > This message is intended solely for the designated recipient(s). It may > contain confidential or proprietary information and may be subject to > attorney-client privilege or other confidentiality protections. If you are > not a designated recipient you may not review, copy or distribute this > message. If you receive this in error, please notify the sender by reply > e-mail and delete this message. Thank you. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytology gross descriptions
Hi I'm looking for a good reference for my grossers to follow while describing fluids. Verbage for color, clarity and viscosity. Anyone have anything that could be useful?? Sent from Xfinity Connect Application ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing correction
Recovering Tissue That Has Dried Out After Malfunction of Tissue Processor | | | | || | | | | | Recovering Tissue That Has Dried Out After Malfunction of Tissue Processor | | | | Sent from Yahoo Mail for iPhone On Monday, March 1, 2021, 2:10 PM, Charles Riley via Histonet wrote: We had an issue last week where the tech loading the processor forgot to start the machine. The tissues we left in an empty chamber for around 8/9 hours after having been in formalin from anywhere from 3 hours to 10 hours from the surgical sites. All of the specimens are unreadable. What steps can be taken (if any) to try and salvage the samples and get better slides for the pathologists to read? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing correction
We had an issue last week where the tech loading the processor forgot to start the machine. The tissues we left in an empty chamber for around 8/9 hours after having been in formalin from anywhere from 3 hours to 10 hours from the surgical sites. All of the specimens are unreadable. What steps can be taken (if any) to try and salvage the samples and get better slides for the pathologists to read? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Any comments on your usage of the following slide scanners?
Hello Histonetters, I am looking for some straight forward comments on a few slide scanners: * Zeiss Z1, * AkoyaBio Vectra Polaris and the * 3DHisTech P250FL. * I do almost exclusively immunofluourescent multiplexing, generally with 4-5 targets and am looking at these scanners. I have had virtual demo's but no live demos and am seeking EXCELLENT image quality as I will need to be quantifying several targets within a sample. Just hoping if you are using one of these or have in the past that you can give any good feedback on your experience with image quality. You can also email me directly if you prefer either to my personal email below or to my work email that is on this post. Thank you! Best regards, Katherine Bummer bumkat0...@gmail.com This message is intended solely for the designated recipient(s). It may contain confidential or proprietary information and may be subject to attorney-client privilege or other confidentiality protections. If you are not a designated recipient you may not review, copy or distribute this message. If you receive this in error, please notify the sender by reply e-mail and delete this message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
