Re: [Histonet] IHC with zamboni's fixed tissues

[Tony Henwood (SCHN) via Histonet]

Hi Colleen,

I was involved in an Immunohistochemical study of the nerves in the human 
ureter that used Zamboni's fixative and cryostat sections. This study was 
before the invention of HIER.

If you are using Zamboni-fixed, paraffin embedded tissue then you will probably 
need to do HIER before many of the localisation antibody incubations since the 
fixative contains 2% formaldehyde. The same might be the case with some 
antibodies when applied to cryostat sections.

Edyvane, K. A., Trussell, D. C., Jonavicius, J., Henwood, A., & Marshall, V. R. 
(1992). Presence and regional variation in peptide-containing nerves in the 
human ureter. Journal of the autonomic nervous system, 39(2), 127-137.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal 
Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of 
Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead 
NSW 2145, AUSTRALIA 




-Original Message-
From: Colleen Forster via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, 26 March 2021 9:42 AM
To: histonet-request 
Subject: [Histonet] IHC with zamboni's fixed tissues

Hello Histonet,

Can you give me feedback on trying to do IHC on samples fixed in Zamboni's 
fixative? I have an idea but need confirmation .

Thank you in advance

Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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[Histonet] IHC with zamboni's fixed tissues

[Colleen Forster via Histonet]

Hello Histonet,

Can you give me feedback on trying to do IHC on samples fixed in Zamboni's
fixative? I have an idea but need confirmation .

Thank you in advance

Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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Re: [Histonet] New antibody diluent

[Terri Braud via Histonet]

CAP specifically addresses Scott's question in the checklist (see below, copied 
from the Checklist)
ANP.22780
Laboratories confirm assay performance when conditions change that may affect 
performance.
NOTE: A change in antibody clone requires full revalidation/verification of the 
assay (equivalent to initial analytic validation/verification - see ANP.22750).
Laboratories must confirm assay performance with at least when an existing 
validated/verified assay has changed in any of the following ways: antibody 
dilution, antibody vendor (same clone), or the incubation or retrieval times 
(same method).

So, according to CAP, each antibody with the new diluent would have to confirm 
with at least two known positive and two known negative cases.
What a pain! (IMHO)

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
HNL Labs, Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

   3. Re: Antibody diluent (Lindrud, Scott)

Message: 3
Date: Thu, 25 Mar 2021 12:30:25 +
From: "Lindrud, Scott" 
Subject: Re: [Histonet] Antibody diluent

What is everyone's take on what needs to be done from a verification/validation 
standpoint if you switch diluents for your concentrated antibodies?  The CAP 
summary of recommendations on the validation IHC antibodies doesn't 
specifically mention the changing of diluent as one of the variables.
If I change the diluent on my ER and Her2 concentrates, do I need to do a 
complete re-validation or just a quick verification using a few positive and 
negative cases?  I'm not really liking the idea of a complete re-validation!
Thanks for any info!
Scott
Scott A. Lindrud, MLSCM(ASCP)CTCM  | Histopathology Technical 
Specalist/Cytotechnologist
P: 320-231-4520
F: 320-231-4503
scott.lind...@carrishealth.com

Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201



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[Histonet] Changing decal question

[Wooten, Jennifer via Histonet]

Good afternoon,
I would like to inquire what everyone's decal (RBD and/or Decal I) changing 
routines are. We are trying to determine if we should continue to change 
weekly, or based on how many specimens we put in it, or what the pH is. Any 
help is greatly appreciated, thanks!

Jennifer Wooten, BA, BS, HTL (ASCP)CM
Pronouns: she/her/hers
Technical Supervisor | Pathology | University Hospital
jennifer.woo...@tricore.org
Desk: 505.272.5486 | Fax: 505.272.0240
TriCore Reference Laboratories
2211 Lomas Blvd NE
Albuquerque, NM 87106
www.tricore.org


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[Histonet] RELIA HOT Histology Opportunity Update - RELIA EXCLUSIVES!!

[Pam Barker via Histonet]

Hi Histopeeps!
I hope you are having a great week.  I wanted to drop a quick post because I
have some really exciting opportunities to share.
Why are they exciting?
1.  Beautiful locations
2.  Day shift hours
3.  Excellent compensation packages
4.  They are ready to interview and HIRE!
And Most importantly - these are RELIA Exclusives!
Here they are:
FL - Ft. Myers - IHC Specialist
OR - Bend  - Histology Tech certification NOT required!!
CA - San Diego - Histotechs - BRAND NEW LAB!

I also have fantastic opportunities in:
TX - Austin
CA - Orange County
NC - Fayetteville
NC - Concord
PA - Lancaster
PA - Chambersburg
CO - Denver
TN - Chattanooga
TN - Nashville
GA - Atlanta

All of the clients that I represent offer full time permanent positions,
excellent compensation and benefits and in most cases relocation/sign on
bonuses.
If you or anyone you know would like more info on these opportunities -
Contact me - Pam Barker - rel...@earthlink.net OR cell/text (407)353-5070.



Thanks-Pam

Right Time, Right Place, Right Move with RELIA!
Providing excellent service exclusively to the Histology Community!

Thank You!
 Pam M. Barker 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net   
https://www.facebook.com/RELIASolutionsforhistologyprofessionals 
www.facebook.com/PamBarkerRELIA 
www.linkedin.com/in/reliasolutions 
www.twitter.com/pamatrelia   
check out our latest opportunities at:
http://www.jobvertise.com/members/relia1 
#jobs4myhistopeeps 
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!



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[Histonet] Positive control blocks for Helicobacter pylori

[Rhonda Ford via Histonet]

Good morning.  I would like to obtain positive control blocks for
Helicobacter pylori.  Thanks in advance for your help.

-- 

Rhonda Ford, Histology Lab
Henry Community Health
Main Campus
1000 North 16th Street
New Castle, Indiana  47362
(765) 521-1148

-- 
*"We Make Lasting Connections"*Henry Community Health
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Re: [Histonet] Antibody diluent

[Patpxs via Histonet]

I’m not completely sure but I think you can do either a mini validation or a 
comparison validation to show there’s no difference between the results.   

I think CAP has a requirement for what to do when reagents change.  

Paula

Sent from my iPhone

> On Mar 25, 2021, at 6:19 AM, Jeanine Ronkowski via Histonet 
>  wrote:
> 
> Scott:I’m pretty certain that you wouldn’t need to completely re-validate 
> after changing diluents, but you may have to change the dilution factor 
> slightly.  It’s my understanding that a change like this only requires 
> staining a few known positive samples, verifying that the results you obtain 
> are very similar if not identical to previous results.  While taking someone 
> else’s suggestion to download that paper on diluents from Biocare’s site, I 
> came across a paper on the subject of validation/verification that I found 
> interesting.Jeanine
> 
> 
> Sent from Yahoo Mail for iPhone
> 
> 
> On Thursday, March 25, 2021, 8:30 AM, Lindrud, Scott 
>  wrote:
> 
> What is everyone's take on what needs to be done from a 
> verification/validation standpoint if you switch diluents for your 
> concentrated antibodies?  The CAP summary of recommendations on the 
> validation IHC antibodies doesn't specifically mention the changing of 
> diluent as one of the variables.
> 
> If I change the diluent on my ER and Her2 concentrates, do I need to do a 
> complete re-validation or just a quick verification using a few positive and 
> negative cases?  I'm not really liking the idea of a complete re-validation!
> 
> Thanks for any info!
> 
> Scott
> 
> 
> Scott A. Lindrud, MLSCM(ASCP)CTCM  | Histopathology Technical 
> Specalist/Cytotechnologist
> 
> P: 320-231-4520
> F: 320-231-4503
> scott.lind...@carrishealth.com
> 
> Rice Memorial Hospital
> 301 Becker Ave SW
> Willmar, MN 56201
> 
> 
> 
> 
> -Original Message-
> From: Jeanine Ronkowski 
> Sent: Tuesday, March 23, 2021 2:21 PM
> To: Paula Keene Pierce ; Maria Cruz 
> ; Morken, Timothy 
> Cc: Histonet 
> Subject: Re: [Histonet] Antibody diluent
> 
> All:Our lab uses a ton of concentrated antibodies and we’ve found that 
> there’s really no such thing as a universal diluent like the ones offered by 
> Leica and Agilent.  We get ours from Biocare, they offer five different 
> diluents with different pHs and chemical makeups and we use three of these. I 
> checked with my Biocare rep and iit seems that the one that’s most similar to 
> Leicas is called Monet Blue.  I suggest you contact your Bocare rep and see 
> if they can send you a sample of one or more them. Good  luck,Jeanine
> 
> 
> Sent from Yahoo Mail for iPhone
> 
> 
> On Tuesday, March 23, 2021, 2:43 PM, Paula Keene Pierce via Histonet 
>  wrote:
> 
> I just purchased antibody diluent from Electron Microscopy Sciences.
> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
> Blue Lake DriveNorman, OK 73069PH 
> 405-759-3953http://www.excaliburpathology.com
> 
> A sharp knife is nothing without a sharp eye. - Klingon Proverb
> 
> On Tuesday, March 23, 2021, 01:36:12 PM CDT, Morken, Timothy via Histonet 
>  wrote:
> 
> Maria, we went back to using Dako Diluent, which we had just switched away 
> from to save money!
> 
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
> Pathology UC San Francisco Medical Center
> 
> -Original Message-
> From: Maria Cruz via Histonet 
> Sent: Tuesday, March 23, 2021 10:55 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Antibody diluent
> 
> Like other folks in the histo community, I’ve recently experienced the 
> inability to buy diluent from Leica.  Now wondering what others are doing to 
> handle this problem.  TIA!
> Maria
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> 
> 
> 
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> confidential information that is legally privileged. This information is 
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> authorized recipient of this information is prohibited from disclosing this 
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[Histonet] Mailing List

[Baumann, Carolyn via Histonet]

Hello,

Kindly remove me for the Histonet mailing list.  I've attempted several
times with no success.

Thank You,

*Carolyn Baumann MT(ASCP)*
Regional Product Specialist-Molecular Diagnostics
Pacific Northwest/Mountain Region

Roche Diagnostics Corporation
Molecular Diagnostic Sales
9115 Hague Road
Indianapolis, IN 46256

Phone:  949-275-8473
carolyn.baum...@roche.com

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Re: [Histonet] Antibody diluent

[Jeanine Ronkowski via Histonet]

Scott:I’m pretty certain that you wouldn’t need to completely re-validate after 
changing diluents, but you may have to change the dilution factor slightly.  
It’s my understanding that a change like this only requires staining a few 
known positive samples, verifying that the results you obtain are very similar 
if not identical to previous results.  While taking someone else’s suggestion 
to download that paper on diluents from Biocare’s site, I came across a paper 
on the subject of validation/verification that I found interesting.Jeanine


Sent from Yahoo Mail for iPhone


On Thursday, March 25, 2021, 8:30 AM, Lindrud, Scott 
 wrote:

What is everyone's take on what needs to be done from a verification/validation 
standpoint if you switch diluents for your concentrated antibodies?  The CAP 
summary of recommendations on the validation IHC antibodies doesn't 
specifically mention the changing of diluent as one of the variables.

If I change the diluent on my ER and Her2 concentrates, do I need to do a 
complete re-validation or just a quick verification using a few positive and 
negative cases?  I'm not really liking the idea of a complete re-validation!

Thanks for any info!

Scott


Scott A. Lindrud, MLSCM(ASCP)CTCM  | Histopathology Technical 
Specalist/Cytotechnologist

P: 320-231-4520
F: 320-231-4503
scott.lind...@carrishealth.com

Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201




-Original Message-
From: Jeanine Ronkowski 
Sent: Tuesday, March 23, 2021 2:21 PM
To: Paula Keene Pierce ; Maria Cruz 
; Morken, Timothy 
Cc: Histonet 
Subject: Re: [Histonet] Antibody diluent

All:Our lab uses a ton of concentrated antibodies and we’ve found that there’s 
really no such thing as a universal diluent like the ones offered by Leica and 
Agilent.  We get ours from Biocare, they offer five different diluents with 
different pHs and chemical makeups and we use three of these. I checked with my 
Biocare rep and iit seems that the one that’s most similar to Leicas is called 
Monet Blue.  I suggest you contact your Bocare rep and see if they can send you 
a sample of one or more them. Good  luck,Jeanine


Sent from Yahoo Mail for iPhone


On Tuesday, March 23, 2021, 2:43 PM, Paula Keene Pierce via Histonet 
 wrote:

I just purchased antibody diluent from Electron Microscopy Sciences.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb

    On Tuesday, March 23, 2021, 01:36:12 PM CDT, Morken, Timothy via Histonet 
 wrote:

 Maria, we went back to using Dako Diluent, which we had just switched away 
from to save money!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

-Original Message-
From: Maria Cruz via Histonet 
Sent: Tuesday, March 23, 2021 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody diluent

Like other folks in the histo community, I’ve recently experienced the 
inability to buy diluent from Leica.  Now wondering what others are doing to 
handle this problem.  TIA!
Maria
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Re: [Histonet] Antibody diluent

[Daniel Pesino via Histonet]

Hi Scott,

It may be best to call CAP to get an interpretation. However, I think that 
since you would be changing diluent for predictive markers, a full validation 
may be required.

Best,

Dan

Daniel Pesino, HTL(ASCP)QIHCCM
Senior Histotechnologist
Trinity Health Of New England
daniel.pes...@trinityhealthofne.org
W  860-714-4675

114 Woodland Street
Hartford, CT 06105
TrinityHealthOfNE.org | Facebook | Twitter | Instagram





-Original Message-
From: Lindrud, Scott via Histonet  
Sent: Thursday, March 25, 2021 8:30 AM
To: Jeanine Ronkowski ; Paula Keene Pierce 
; Maria Cruz ; Morken, 
Timothy 
Cc: Histonet 
Subject: [External] Re: [Histonet] Antibody diluent

Warning:  This email originated from the Internet!
DO NOT CLICK links if the sender is unknown, and NEVER provide your password.

What is everyone's take on what needs to be done from a verification/validation 
standpoint if you switch diluents for your concentrated antibodies?  The CAP 
summary of recommendations on the validation IHC antibodies doesn't 
specifically mention the changing of diluent as one of the variables.

If I change the diluent on my ER and Her2 concentrates, do I need to do a 
complete re-validation or just a quick verification using a few positive and 
negative cases?  I'm not really liking the idea of a complete re-validation!

Thanks for any info!

Scott


Scott A. Lindrud, MLSCM(ASCP)CTCM  | Histopathology Technical 
Specalist/Cytotechnologist

P: 320-231-4520
F: 320-231-4503
scott.lind...@carrishealth.com

Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201




-Original Message-
From: Jeanine Ronkowski 
Sent: Tuesday, March 23, 2021 2:21 PM
To: Paula Keene Pierce ; Maria Cruz 
; Morken, Timothy 
Cc: Histonet 
Subject: Re: [Histonet] Antibody diluent

All:Our lab uses a ton of concentrated antibodies and we’ve found that there’s 
really no such thing as a universal diluent like the ones offered by Leica and 
Agilent.  We get ours from Biocare, they offer five different diluents with 
different pHs and chemical makeups and we use three of these. I checked with my 
Biocare rep and iit seems that the one that’s most similar to Leicas is called 
Monet Blue.  I suggest you contact your Bocare rep and see if they can send you 
a sample of one or more them. Good  luck,Jeanine


Sent from Yahoo Mail for iPhone


On Tuesday, March 23, 2021, 2:43 PM, Paula Keene Pierce via Histonet 
 wrote:

I just purchased antibody diluent from Electron Microscopy Sciences.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb

On Tuesday, March 23, 2021, 01:36:12 PM CDT, Morken, Timothy via Histonet 
 wrote:

 Maria, we went back to using Dako Diluent, which we had just switched away 
from to save money!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

-Original Message-
From: Maria Cruz via Histonet 
Sent: Tuesday, March 23, 2021 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody diluent

Like other folks in the histo community, I’ve recently experienced the 
inability to buy diluent from Leica.  Now wondering what others are doing to 
handle this problem.  TIA!
Maria
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Re: [Histonet] Antibody diluent

[Lindrud, Scott via Histonet]

What is everyone's take on what needs to be done from a verification/validation 
standpoint if you switch diluents for your concentrated antibodies?  The CAP 
summary of recommendations on the validation IHC antibodies doesn't 
specifically mention the changing of diluent as one of the variables.

If I change the diluent on my ER and Her2 concentrates, do I need to do a 
complete re-validation or just a quick verification using a few positive and 
negative cases?  I'm not really liking the idea of a complete re-validation!

Thanks for any info!

Scott


Scott A. Lindrud, MLSCM(ASCP)CTCM  | Histopathology Technical 
Specalist/Cytotechnologist

P: 320-231-4520
F: 320-231-4503
scott.lind...@carrishealth.com

Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201




-Original Message-
From: Jeanine Ronkowski 
Sent: Tuesday, March 23, 2021 2:21 PM
To: Paula Keene Pierce ; Maria Cruz 
; Morken, Timothy 
Cc: Histonet 
Subject: Re: [Histonet] Antibody diluent

All:Our lab uses a ton of concentrated antibodies and we’ve found that there’s 
really no such thing as a universal diluent like the ones offered by Leica and 
Agilent.  We get ours from Biocare, they offer five different diluents with 
different pHs and chemical makeups and we use three of these. I checked with my 
Biocare rep and iit seems that the one that’s most similar to Leicas is called 
Monet Blue.  I suggest you contact your Bocare rep and see if they can send you 
a sample of one or more them. Good  luck,Jeanine


Sent from Yahoo Mail for iPhone


On Tuesday, March 23, 2021, 2:43 PM, Paula Keene Pierce via Histonet 
 wrote:

I just purchased antibody diluent from Electron Microscopy Sciences.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb

On Tuesday, March 23, 2021, 01:36:12 PM CDT, Morken, Timothy via Histonet 
 wrote:

 Maria, we went back to using Dako Diluent, which we had just switched away 
from to save money!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

-Original Message-
From: Maria Cruz via Histonet 
Sent: Tuesday, March 23, 2021 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody diluent

Like other folks in the histo community, I’ve recently experienced the 
inability to buy diluent from Leica.  Now wondering what others are doing to 
handle this problem.  TIA!
Maria
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