Re: [Histonet] "cooked" biopsy
Hi Paula, We can check the purity of the xylene quite easily: Xylene Purity Test Procedure Note: The recommended and most accurate method of determining the purity of the recycled xylene is by doing a Gas Chromatography analysis. The following method can be used to obtain an acceptable confidence level in the purity of the recycled xylene (CBG Biotech). Testing Procedure 1. To a clean, dry 100 ml mixing cylinder graduate, add sufficient recovered xylene so that the bottom of the meniscus is aligned with the top edge of the 85 ml mark on the graduate. 2. Add water to the graduate until the bottom of the meniscus aligns with the top edge of the 100 ml mark on the graduate. At this point, 15 ml of water will have been added to 85 ml of recovered xylene. 3. Stopper the graduate and invert the mixture. Allow the mixture to settle, making sure that all of the water settles to the bottom of the graduate. No water should remain clinging to the sides of the graduate above the xylene/water separation point. This separation point should be near the 15 ml level of the graduate. (Note: xylene floats on top of the water). 4. Carefully inspect and record the point of separation between the water and xylene using the bottom of the meniscus as the separation point. 5. Subtract 15 ml from the quantity of water indicated in step 5. The remainder plus an additional 0.1 correction factor equals the percentage of recovered xylene impurities. EXAMPLE: Xylene/Water separation point is indicated to be 15.5 ml. (15.5 - 15) + 0.1 = 0.6% impurities. Therefore, the recovered xylene is 99.4% pure. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Paula via Histonet Sent: Thursday, 8 July 2021 04:53 To: 'Erick Rodriguez' Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] "cooked" biopsy Thank you, everyone... I looked at my reagents and saw the color pink in the xylene, which tells me that there is water or too much water in it so I changed it. We recycle xylene, so I need to get the recycler looked at now. Thanks again, Paula -Original Message- From: Erick Rodriguez [mailto:rodriguez.er...@icloud.com] Sent: Wednesday, July 07, 2021 11:24 AM To: Paula Subject: Re: [Histonet] "cooked" biopsy Did you change the processor reagents before running your tissues? Cooked tissue usually means the tissue wasn’t dehydrated properly and the leftover water boiled and fried your tissue. I would double check the alcohols. > On Jul 7, 2021, at 11:00 AM, Paula via Histonet > wrote: > > Hello, good day, > > > > Our pathologist is complaining about the tissues today that they are > "cooked, burnt, crushed, shrunken" those are the adjectives she is using. > > > > Can you tell me the cause? Usually, the work comes out beautiful but today > they are not. Nothing has changed on our processing times. > > > > What should I investigate? > > > > Thank you in advance, > > Paula > > Bio-Path Medica Group > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotechnician/Grosser Needed Long Island NY
Good Afternoon- New permanent histology opening! *Histotechnician/Grosser Needed Long Island, NY* - Permanent full time position - Shift is Tuesday to Saturday 6:00am - 2:30pm but seems to have flexibility - Candidates must have New York State Clinical Laboratory Technologist/Technician or Histological Technician License - Ideally would like to see candidates with 1+ year of experience - In this role you will embed, cut, stain and coverslip specimens. Set up specimens and their paperwork for grossing. Maintain the tissue processor, embedding centers, microtomes, stainers and coverslipper, etc. - Full benefits included and much more!!! - Position is located on Western Long Island- only about 35 miles from Manhattan. If you would like to talk further about these openings I am working on please send an updated resume to oli...@ka-recruiting.com with the best time and phone number for me to reach you! Or feel free to reach out to my direct line which is 617-746-2743 . You can also schedule an appointment with my calendar: https://calendly.com/olivia-ka/15min *Other Permanent Histology Openings Nationwide* FL - Tampa Area- Histology Manager GA – 1.5 Hours South of Atlanta - Histotech (3 am - 11 am) NC – Winston-Salem Area - Histotech NY- Bronx Area- Histotech NY - Buffalo Area- CLT Histology Lab Pathology NY - Westchester - Histotech NY - Westchester - IHC Lab Tech (Histotech) NY - Westchester - IHC Lead Tech (Histotech) NY – Syracuse Area - Histotech NY – Long Island - Histotechnician or Histotechnologist NY - Rochester Area- Histotechnologist (Day shift) OR - Northwest- Grossing Histotech OR - Northwest - Histotech PA - Southeast - Histotechnologist PA - Southeast - Histotech TN - Nashville Area- Histotech VA - Southeast - Senior Histotech VA - Southeast - Histotech VA – Roanoke Area - Histotech WI – Greater Green Bay Area - Histotech WI – Greater Green Bay Area - Lab Histotech Trainer Talk to you soon, Olivia Sloane Healthcare Recruiter, K.A. Recruiting, Inc. * 617-746-2743 * oli...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Iron stain on Bone Marrow crush specimens
Hello, Would anyone be willing to share their protocol for performing iron stains on bone marrow crush specimens? We are trying to convert from doing the stain manually to automated on a Ventana Special Stainer using the iron stain kit from Roche. We are using the following protocol: 1. Fix slide in methanol x 2 minutes 2. Allow slide to air dry 3. Rinse slide in distilled water 4. Place slide on special stainer (Ventana only allows adjusting counterstain time, currently set at 4 minutes) My plan is to pull additional crush smears and trying different counterstain times, but if anyone has other suggestions, it would be greatly appreciated. Best Regards, Carole Carole L Johnson, HT, QIHC (ASCP) CONFIDENTIALITY NOTICE: This message and any attachments may contain privileged and confidential peer review, risk management, and/or quality management information pursuant to the Colorado Professional Review Act, C.R.S. 12-36.5-101, et seq., the Colorado Hospital Licensing law, C.R.S. 25-3-109, the Quality Management Programs law, C.R.S. 25-35-904, and other corresponding provisions of federal and state law. Please maintain the strict confidentiality of this information. If you are not the intended recipient, you are notified that any disclosure, copying, distribution, electronic storage or use of this communication is prohibited. If you received this communication in error, please notify us immediately by e-mail, attaching the original message, and delete the original message from your computer and any network to which your computer is connected. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] "cooked" biopsy
Thank you, everyone... I looked at my reagents and saw the color pink in the xylene, which tells me that there is water or too much water in it so I changed it. We recycle xylene, so I need to get the recycler looked at now. Thanks again, Paula -Original Message- From: Erick Rodriguez [mailto:rodriguez.er...@icloud.com] Sent: Wednesday, July 07, 2021 11:24 AM To: Paula Subject: Re: [Histonet] "cooked" biopsy Did you change the processor reagents before running your tissues? Cooked tissue usually means the tissue wasn’t dehydrated properly and the leftover water boiled and fried your tissue. I would double check the alcohols. > On Jul 7, 2021, at 11:00 AM, Paula via Histonet > wrote: > > Hello, good day, > > > > Our pathologist is complaining about the tissues today that they are > "cooked, burnt, crushed, shrunken" those are the adjectives she is using. > > > > Can you tell me the cause? Usually, the work comes out beautiful but today > they are not. Nothing has changed on our processing times. > > > > What should I investigate? > > > > Thank you in advance, > > Paula > > Bio-Path Medica Group > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] "cooked" biopsy
Hello, good day, Our pathologist is complaining about the tissues today that they are "cooked, burnt, crushed, shrunken" those are the adjectives she is using. Can you tell me the cause? Usually, the work comes out beautiful but today they are not. Nothing has changed on our processing times. What should I investigate? Thank you in advance, Paula Bio-Path Medica Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
