Re: [Histonet] Vacuum packing of formalin fixed tissues for archiving

[Tony Henwood (SCHN) via Histonet]

Hi Michelle,

We have used the Milestone Tissue Safe Plus unit with Integrated Data Logger 
card reader to seal formalin-fixed placentas since 2019.
Placentas are drained of excess formalin in a chemical hood, placed into a 
labelled bag and sealed.

We instituted the process to reduce the formalin fume leakage that often 
accompanies the storage of these specimens and to decrease the storage space 
required.

The process has lived up to its aims (formalin fume containment, no liquid 
leakage and reduced storage requirements) and we are currently sampling the 
early specimens (3 years in storage) to analyse any adverse morphology and 
Immunohistochemical staining that might occur.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: Michelle Perrins via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, 6 October 2021 11:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Vacuum packing of formalin fixed tissues for archiving

Hello
I would like to know if any histology laboratory stores their fixed specimens 
this way and what instrument do you use?
Regards
Michelle

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Re: [Histonet] GLUT-1 and Desmin

[Maria Cruz via Histonet]

The lab I work gets both of these antibodies from Biocare, and we rarely,
if ever, experience a back order from that vendor.

On Wed, Oct 6, 2021 at 1:06 PM Pairan, Kelly via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Good Afternoon,
> Our current vendor of GLUT-1 and Desmin has a backorder until next year.
> Would you mind sharing what your institutions use?  We run them on Leica
> Bonds.
>
> Thanks,
> Kelly
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[Histonet] Dry Film Coverslipping?

[Cates, Julia via Histonet]

Hello Histonet,

I have been asked to consider going Xylene free.  The only hang up that I am 
having is the coverslipping part.  We have an old Sakura Film coverslipper and 
I don't know how that would work without xylene.  When I did some searching in 
the archives, I found some comments made by Rene, that he used this type of 
coverslipper on oven dried slides but never referenced how.  Does anyone use 
this method in your lab?  If so, would you share your protocol and are there 
any problems to consider?

Thanks,

Julia C.


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[Histonet] GLUT-1 and Desmin

[Pairan, Kelly via Histonet]

Good Afternoon,
Our current vendor of GLUT-1 and Desmin has a backorder until next year.  Would 
you mind sharing what your institutions use?  We run them on Leica Bonds.

Thanks,
Kelly
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[Histonet] Vacuum packing of formalin fixed tissues for archiving

[Michelle Perrins via Histonet]

Hello
I would like to know if any histology laboratory stores their fixed specimens 
this way and what instrument do you use?
Regards
Michelle

Sent from Mail for Windows

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email disclaimer published on our website at 
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If this email is not related to the business of UCT, it is sent by the sender 
in an individual capacity. Please report security incidents or abuse via 
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