[Histonet] New opening
Hi Histonetters, I just got in an exciting new job opportunity! I am working with a lab in Southern Connecticut that is looking to hire a *Manager of Anatomic Pathology, Molecular Pathology, and Digital Pathology*. My client needs someone who understands these three areas and who can support the transition in the lab from only derm to doing more. It is a permanent, full-time position. If you are interested, could you send me a resume and a good time for me to give you a call? If not, feel free to forward my info along to anyone you think could be a good fit! Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 kather...@ka-recruiting.com http://www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] tissue disposal
Hi Pam, We have used the Milestone Tissue Safe Plus unit with Integrated Data Logger card reader to seal formalin-fixed placentas (and other specimens if needed) since 2019. Placentas are drained of excess formalin in a chemical hood, placed into a labelled bag and sealed. So possibly for long term storage, the Tissue Safe Plus might be worth looking at. To save the environment, we try and re-cycle the larger plastic containers. As clean as possible but we try to not spend too much time on them. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: Richardson, Pam K via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, 14 October 2021 7:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue disposal I am interested to learn how others are handling tissue disposal. How long to you keep wet tissue? Are you reusing containers? We currently dispose of the tissue and wash the containers and I am questioning if there is a better system? Best Wishes Pam ~ National Histology Professionals Day 3/10/21 Pathologists' Assistant Day 4/14/2021 Medical Laboratory Professionals Week April 18-24, 2021 National Cytotechnology Day 5/13/2021 +++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkric...@gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://secure-web.cisco.com/1NGyXQUNr0j3MPQIjvmHqDwbUHBOGTSAJGY8v49mWsvp1_J2JSv0VL0YU2v5fBMwm9P9gkTaqYKmqnmP-QuNOClh6woo0CZiF0odkeVPTcoU5KS5ryxDxp16U_vnAsVLijWu1CwBGDPkJLePBE4OKDeXzL3cX-bzf0r-dKm0B9hw9e_yFECTUr-x3L_AVKV3OEhuj1kxg-ohIeIRtgqr4e0OlWI1MflK2moiDwp3XHYOx1kk8j4WPwEsuQqG1sLi6FKEDmyeUND1yNAGvWc5mhpyyyEHi9-3_9hd-8JUIk379SYu2qAHtKWCIdXHaovxUfgrnfqQp4X8G2t6pER6kt9RMNNAg7H_Kp9rl_V_SspLOu3QVAddkglNqSBHC3oFxLtxU5-HyiKOdHf5WqYOXdldkvybWkSXOf78Vl0gzJyIE4_lFYSwx26nqcd78P3vMVctVFNiNZry0Yo0oU6m-TjQlve8-a6kR98wk7nR04dQ/http%3A%2F%2Fwww.gundersenhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] tissue disposal
We dumped tissue two after the final report is issued and toss the containers. Sent from Yahoo Mail for iPad On Wednesday, October 13, 2021, 3:36 PM, Richardson, Pam K via Histonet wrote: I am interested to learn how others are handling tissue disposal. How long to you keep wet tissue? Are you reusing containers? We currently dispose of the tissue and wash the containers and I am questioning if there is a better system? Best Wishes Pam ~ National Histology Professionals Day 3/10/21 Pathologists' Assistant Day 4/14/2021 Medical Laboratory Professionals Week April 18-24, 2021 National Cytotechnology Day 5/13/2021 +++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkric...@gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] tissue disposal
I am interested to learn how others are handling tissue disposal. How long to you keep wet tissue? Are you reusing containers? We currently dispose of the tissue and wash the containers and I am questioning if there is a better system? Best Wishes Pam ~ National Histology Professionals Day 3/10/21 Pathologists' Assistant Day 4/14/2021 Medical Laboratory Professionals Week April 18-24, 2021 National Cytotechnology Day 5/13/2021 +++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkric...@gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reprocessing Protocol
It makes no sense to heat a paraffin-infiltrated specimen in saline. Sodium chloride isn't soluble in hot paraffin or in any of the organic solvents used in tissue processing. Heating in water may melt and float out all the wax and rehydrate a specimen, but does it matter if some wax remains? Reprocessing is an attempt to correct the effects of incomplete dehydration. This can be done by taking the specimen back into the clearing agent (xylene or similar) and then into 2 changes of 100% alcohol (methyl, ethyl or isopropyl). Why rehydrate? For a rehydrated specimen, why go "from formalin, using a schedule that would have been of appropriate length as used initially"? This will surely produce, again, a block that is incompletely dehydrated. John Kiernan London, Canada = = = From: Etheridge, Sandra AFF:EX via Histonet Sent: October 12, 2021 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Reprocessing Protocol Hi Curt, We have used Taggart's Method quite successfully in the past for poorly processed tissues. You can find it online. The isotonic saline may help to rehydrate your tissues. 1. Melt down the tissue block in the embedding centre block tray area and gently blot off the excess wax. Place the tissue in a newly labelled cassette. 2. Place the cassette into a beaker of isotonic saline (0.9% sodium chloride) and place it in the 65 C incubator/oven for one hour. This will melt the residual wax which will rise to the surface of the saline. 3. Remove the cassette from the saline, drain briefly and place in your processor from formalin, using a schedule that would have been of appropriate length as used initially. 4. Embed and section as per usual. Good luck! Sandra Etheridge -Original Message- From: histonet-requ...@lists.utsouthwestern.edu Sent: October 12, 2021 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 215, Issue 8 [EXTERNAL] This email came from an external source. Only open attachments or links that you are expecting from a known sender. Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=04%7C01%7Csandra.etheridge%40gov.bc.ca%7Cbdb6ae0c78de43df498d08d98da1c4da%7C6fdb52003d0d4a8ab036d3685e359adc%7C0%7C0%7C637696548191633375%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000sdata=BrEkw92hcrZCE2E7LfNRrM3Zi%2BIxWyNUSevhTPcXqdQ%3Dreserved=0 or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. reprocessing tissue (Curt Tague) -- Message: 1 Date: Tue, 12 Oct 2021 15:14:54 + From: Curt Tague To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] reprocessing tissue Message-ID: Content-Type: text/plain; charset="us-ascii" I have a problem... some tissue got processed very poorly, there was water in the system somewhere and a few blocks just look burnt.. the nuclei are faint and cloudy, no detail at all. I've tried the process of rehydrating with the 30% formaldehyde, glycerol and sodium acetate solution but they still process poorly, come out very brittle and just don't look good under the scope. Does anyone have a magic bullet to salvage these specimens? I can send a pic directly if it helps. Thanks, Curt -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=04%7C01%7Csandra.etheridge%40gov.bc.ca%7Cbdb6ae0c78de43df498d08d98da1c4da%7C6fdb52003d0d4a8ab036d3685e359adc%7C0%7C0%7C637696548191633375%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000sdata=BrEkw92hcrZCE2E7LfNRrM3Zi%2BIxWyNUSevhTPcXqdQ%3Dreserved=0 -- End of Histonet Digest, Vol 215, Issue 8 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
