Re: [Histonet] Roll plate, Freeze spray in the Cryostat
Just a word of caution that unless you are quite sure of the cleanliness of your cryostat, or the infectious status of the specimens cut in your cryostat, freezing spray is the worst product to use in a cryostat. It creates aerosolized particles that can linger, and infect, for hours. At our lab, we long ago decided to forbid the use, and we have no problems cutting beautiful frozen sections without it. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 Honesty AccouNtability AgiLity CoLlaboration CoMpassion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] roll plate - using freezing spray in a cryostat
Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 Honesty AccouNtability AgiLity CoLlaboration CoMpassion You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Cryostat anti-roll plate (Ken Marzinsky) 2. Re: Cryostat anti-roll plate (Keyser, Geri L) 3. Re: Cryostat anti-roll plate (Anne Murvosh) 4. Re: Cryostat anti-roll plate (Keyser, Geri L) 5. Re: Cryostat anti-roll plate (Keyser, Geri L) -- Message: 1 Date: Tue, 24 May 2022 20:16:10 + From: Ken Marzinsky To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Cryostat anti-roll plate Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time? I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle. Ken Durham, NC -- Message: 2 Date: Wed, 25 May 2022 12:15:06 + From: "Keyser, Geri L" To: "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] Cryostat anti-roll plate Message-ID: Content-Type: text/plain; charset="us-ascii" I use roll plate. I don't like the roll plate on the Leica. The Leica design creeps... a lot. Also, with the leica, the contact point with the blade is a soft aluminum arm. If you make adjustments with the roll plate holder contacting the blade, it will damage the soft aluminum holder. I like the thermo design much better. It creeps a lot less. Here's a procedure for roll plate adjustment. 1. Mount block on specimen disc. 2. Place Specimen Disc in chuck, position Blade carrier, and face off. 3. Turn knurling screw clockwise until sections do not pass under the Anti-roll plate. 4. Turn knurling screw counter-clockwise by 20 degree increments until sections effortlessly pass under the Anti-Roll Plate WITHOUT hitting the block. * If the block is hitting the anti-roll plate, it may cause damage to glass. * Hard contact is visible and can be heard. * Soft contact, which can also damage the Anti-roll plate, cannot be seen or heard. But, can be felt by gently placing finger on the anti-roll plate turning lever while taking a section. Geri -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time? I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle. Ken Durham, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Wed, 25 May 2022 13:32:12 + From: Anne Murvosh To: Ken Marzinsky , "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] Cryostat anti-roll plate Message-ID: < st them. They creep forward so much that I just automatically know to roll imw4pr15mb45615d24e17f493a42281080c6...@mw4pr15mb4561.namprd15.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" We have 3 new leicas and we constantly have to adju t slightly back after every 4 specimens or so. The new leica designs are just bad and customer service is even worse. We have to use the plate as we do Mohs specimens and the sections are just better with the glass plate. I want to look for a different machine next time. We did test the Avantik and it cut nice but the glass plate was on the front and got in the way. I wish they would talk to Mohs techs before they design these. Oh well. No tricks just constant adjusting. Thanks Anne -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-ro
Re: [Histonet] Cryostat anti-roll plate
> > > My experience with anti-roll plates is mostly with the old International > cryostats of days gone by. Pathologists usually disdained anti-roll plates > and used an artist's brush to keep the section from rolling, but more > proficient users depended on them. I learned to use them in a research > histology lab. We sat at the side of the cryostat by the crank, rather than > in front of it, and used an anti-roll plate. It was easy to cut fifty > slides with it, which I required for my research fifty years ago. Bob Richmond retired samurai pathologist Maryville TN > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat anti-roll plate
Got it... I need to test it. But, I'm going to take some parafilm, pull it over the bolt, then replace the screw. Increase that friction. 😊 Geri -Original Message- From: Keyser, Geri L via Histonet Sent: Wednesday, May 25, 2022 9:59 AM To: Anne Murvosh ; Ken Marzinsky ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryostat anti-roll plate Maybe there's a product that increases friction with screw threads, doesn't have toxic outgassing, and works at low temperatures. I'm going to look for something like this. Geri -Original Message- From: Anne Murvosh via Histonet Sent: Wednesday, May 25, 2022 8:32 AM To: Ken Marzinsky ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryostat anti-roll plate WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. We have 3 new leicas and we constantly have to adjust them. They creep forward so much that I just automatically know to roll it slightly back after every 4 specimens or so. The new leica designs are just bad and customer service is even worse. We have to use the plate as we do Mohs specimens and the sections are just better with the glass plate. I want to look for a different machine next time. We did test the Avantik and it cut nice but the glass plate was on the front and got in the way. I wish they would talk to Mohs techs before they design these. Oh well. No tricks just constant adjusting. Thanks Anne -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time? I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle. Ken Durham, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Camurvosh%40advancederm.net%7Cd00ec7fb978f48d5784b08da3dc25292%7Cc026b5a4e9b54bdfb53b7130122cdb95%7C0%7C0%7C637890202080603264%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=Uhz9rhvCBTV95t%2BNEs0ocizQBXV7aAQyxswaE2Thm2E%3D&reserved=0 HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat anti-roll plate
Maybe there's a product that increases friction with screw threads, doesn't have toxic outgassing, and works at low temperatures. I'm going to look for something like this. Geri -Original Message- From: Anne Murvosh via Histonet Sent: Wednesday, May 25, 2022 8:32 AM To: Ken Marzinsky ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryostat anti-roll plate WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. We have 3 new leicas and we constantly have to adjust them. They creep forward so much that I just automatically know to roll it slightly back after every 4 specimens or so. The new leica designs are just bad and customer service is even worse. We have to use the plate as we do Mohs specimens and the sections are just better with the glass plate. I want to look for a different machine next time. We did test the Avantik and it cut nice but the glass plate was on the front and got in the way. I wish they would talk to Mohs techs before they design these. Oh well. No tricks just constant adjusting. Thanks Anne -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time? I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle. Ken Durham, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Camurvosh%40advancederm.net%7Cd00ec7fb978f48d5784b08da3dc25292%7Cc026b5a4e9b54bdfb53b7130122cdb95%7C0%7C0%7C637890202080603264%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=Uhz9rhvCBTV95t%2BNEs0ocizQBXV7aAQyxswaE2Thm2E%3D&reserved=0 HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat anti-roll plate
We have 3 new leicas and we constantly have to adjust them. They creep forward so much that I just automatically know to roll it slightly back after every 4 specimens or so. The new leica designs are just bad and customer service is even worse. We have to use the plate as we do Mohs specimens and the sections are just better with the glass plate. I want to look for a different machine next time. We did test the Avantik and it cut nice but the glass plate was on the front and got in the way. I wish they would talk to Mohs techs before they design these. Oh well. No tricks just constant adjusting. Thanks Anne -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time? I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle. Ken Durham, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Camurvosh%40advancederm.net%7Cd00ec7fb978f48d5784b08da3dc25292%7Cc026b5a4e9b54bdfb53b7130122cdb95%7C0%7C0%7C637890202080603264%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=Uhz9rhvCBTV95t%2BNEs0ocizQBXV7aAQyxswaE2Thm2E%3D&reserved=0 HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat anti-roll plate
I use roll plate. I don't like the roll plate on the Leica. The Leica design creeps... a lot. Also, with the leica, the contact point with the blade is a soft aluminum arm. If you make adjustments with the roll plate holder contacting the blade, it will damage the soft aluminum holder. I like the thermo design much better. It creeps a lot less. Here's a procedure for roll plate adjustment. 1. Mount block on specimen disc. 2. Place Specimen Disc in chuck, position Blade carrier, and face off. 3. Turn knurling screw clockwise until sections do not pass under the Anti-roll plate. 4. Turn knurling screw counter-clockwise by 20 degree increments until sections effortlessly pass under the Anti-Roll Plate WITHOUT hitting the block. * If the block is hitting the anti-roll plate, it may cause damage to glass. * Hard contact is visible and can be heard. * Soft contact, which can also damage the Anti-roll plate, cannot be seen or heard. But, can be felt by gently placing finger on the anti-roll plate turning lever while taking a section. Geri -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time? I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle. Ken Durham, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
