Re: [Histonet] Manual double staining IHC

[Frazer Bell via Histonet]

Hi,

We have successfully done double staining using both DAB and Magenta on our 
Dako autostainer, you could do it manually.

If you would like further information please let me know.


Frazer Bell
Histopathology Technician

Histology Research Service/ Veterinary Diagnostics Services
307 Jarrett Building
School of Biodiversity, One Health & Veterinary Medicine
College of Medical, Veterinary and Life Sciences University of Glasgow Bearsden 
Road Glasgow
G61 1QH
Tel: +44 (0) 141 330 6933
email: vet-histol...@glasgow.ac.uk
Website: http://www.gla.ac.uk/histology
The University of Glasgow, charity number SC004401



-Original Message-
From: Charles Riley via Histonet  
Sent: 17 November 2022 19:26
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Manual double staining IHC

Hello all,

I have never done double staining manually and wanted to know if anyone has 
protocols or suggestions for doing this?
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[Histonet] Histotechnician and Medical Technologist positions - Fred Hutchinson - Seattle

[Donato, Elizabeth via Histonet]

Hello Everyone,

I hope you will consider sharing these job postings with your colleagues.  Fred 
Hutchinson Cancer Center is a wonderful organization to work for and the 
Clinical Trials Pathology lab in particular is involved in impressive, 
cutting-edge research.

Thank you,

Liz


Research Histotechnician I/II

https://careers-fhcrc.icims.com/jobs/23684/research-histotechnician-i-ii/job


Clinical Research Technologist II

https://careers-fhcrc.icims.com/jobs/23780/clinical-research-technologist-ii/job

www.fredhutch.org



Liz Donato

Manager | Specialized Pathology Laboratory

Clinical Research Division

Fred Hutchinson Cancer Center

•

206.667.4501 p | 425.443.6388 c | 206.667.5255 f | 
edon...@fredhutch.org

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[Histonet] Manual double staining IHC

[Charles Riley via Histonet]

Hello all,

I have never done double staining manually and wanted to know if anyone has
protocols or suggestions for doing this?
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Re: [Histonet] Testing Skills

[Terri Braud via Histonet]

Aside from the terrible legal  liability that you would open your institution 
to by asking them to operate "dangerous" equipment when they are not in your 
employ, sitting at a strange microtome/waterbath set up with different knives, 
paraffins, processing, just would not be an accurate test.
I really liked Thomas Podawiltz's idea.  Usually I just ask leading questions 
and then evaluate during the probation period.

Terri L. Braud, HT(ASCP)
HNL Laboratories for 
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874


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