Hi If you are using the peroxidase method the hydrogen peroxide step will remove the eosin and some of the haematoxylin.
No need to do a decolorisation step before staining. As Gudren said the only problem may be not having coated slides. Good luck. Sadly retired histologist Tony Sent from my iPhone > On 10 Feb 2023, at 6:07 am, Katleba, Maria via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Oh no!!! We cut all slides on + > > BUT you can do this. It’s what we do when we have H&E on plain slides. > > Whether you have a dry unstain from another lab that sent u a slide, or your > H&E that you just took the coverslip off… > > Place it in. Coplin jar with 1-3 DROPS of formalin, cover, place In 60ft > degree oven for 60 min. You want the bottom of the jar to be moist not > floating in it > > After 60 min remove and open UNDER A HOOD (it’s a vaporized carcinogen) > Air out 10-15 min… place on stainer > > The drops vaporize and the aldehyde goes through tissue and make it adhere to > the slide permanently. > > I do this when I cut nails or anything that won’t stay on a plus slide. > > Maria > > Sent from my iPhone > >> On Feb 8, 2023, at 11:21 PM, Gudrun Lang via Histonet >> <histonet@lists.utsouthwestern.edu> wrote: >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
