Re: [Histonet] A question for my hospital lab peeps
Hello Terri, Please see an excerpt from our frozen section manual. For all sentinel lymph nodes and non-sentinel lymph nodes obtained from breast and gynecologic surgeries, entirely submit the lymph node after serially sectioning perpendicular to the long axis at 0.2 cm intervals. It is essential that all unique surfaces be represented, i.e. opposing surfaces should not be embedded in the same direction. How many frozen levels? Seeing as we are serially sectioning the lymph node, we only do a single level. How many permanent section Levels? All remnants are submitted after frozen, again we only perform the microtomy of a single level. Any unstained ffpe sections saved or stained IHC? It is not uncommon for AE1/AE3 to be requested which we then cut the IHC request followed by an additional recut H&E. Thanks Lacy Normington Lacy Normington, HTL(CM) She/Her/Hers Manager, Clinical Laboratories Surgical Pathology, Autopsy 600 Highland Ave, Mail 3224 Madison, WI 53792 Office: 608.890.9373 -Original Message- From: Terri Braud via Histonet Sent: Thursday, February 23, 2023 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A question for my hospital lab peeps WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. I'd love to hear your input On breast mastectomy cases with sentinel lymph nodes, what is your cutting protocol for sentinel lymph nodes sent for frozen section? How many frozen levels? How many permanent section Levels? Any unstained ffpe sections saved or stained IHC? I'm just trying to make sure that what we're doing is the norm. Thanks in advance. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Honesty AccouNtability AgiLity CoLlaboration CoMpassion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7CLnormington%40uwhealth.org%7C39c802fc6114419b6f1508db15ca3212%7C0fd7902a3b4f49b0b1edaaa4d2b4f5f1%7C0%7C0%7C638127730396655742%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=NneLV1tWvVwR7RTukOLBHO4QRmy0EVgPKIg2OzIbAbs%3D&reserved=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] A question for my hospital lab peeps
I'd love to hear your input On breast mastectomy cases with sentinel lymph nodes, what is your cutting protocol for sentinel lymph nodes sent for frozen section? How many frozen levels? How many permanent section Levels? Any unstained ffpe sections saved or stained IHC? I'm just trying to make sure that what we're doing is the norm. Thanks in advance. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Honesty AccouNtability AgiLity CoLlaboration CoMpassion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processors
Has anyone used the Donatello 2 tissue processor from Diapath ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Shandon Elliott Autosharp consumables
I haven't posted anything on here for a while, hope someone can help me as I'm drawing a blank searching on the internet. I'm one of the leaders of an amateur microscopy group in the UK, the Anglian Microscopy Group, and one of the subjects we regularly cover is histology where we help members to make thin sections - usually of plant material - stain and mount them. Quite a few of our members own a Cambridge Rocking microtome as in the past they were very popular with amateur microscopists in the UK. Only problem is that they use a fixed blade and replaceable blade holders are expensive and frankly usually poor quality. We were recently give a very good condition Shandon Elliott Autosharp (think it's a Mark 2) with all the blade holders and four glass plates that we want to use for sharpening Cambridge Rocker blades. It also came with one bottle of abrasive powder, presumably aluminium oxide, but with no sensible indication of grain size. I've been trying to get hold of information on which abrasives should be used and I'm hitting a bit of a blank wall, probably because these machines are effectively obsolete in professional circles. In an older paper I've found the following abrasives were recommended; Aloxite 2F, Aloxite Optical 50, Aloxite 1200 and Polishing Alumina 350, however these are no longer in production and the company that made/marketed them in the UK, the Carborundum Company, Manchester, no longer exists. I don't think the numbers refer to grit sizes, especially the Optical 50. Diamond pastes are unlikely to be of any use to us as they require copper plates that we don't have. Is there anyone out there who can provide me with information on which currently produced aluminium oxide abrasives will be suitable for this machine, preferably with the grit size in micrometres so that that I can look for alternatives? If anyone in the UK actually has any old tins of these materials we'd be very interested in acquiring them! I've given the club email address below if anyone wants to contact us directly. Many thanks Gordon anglian.microsc...@gmail.com www.anglianmc.org.uk ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
