[Histonet] distilled water systems
Not sure what everyone else is doing but we're buying a TON of DI water for bulk IHC reagents... I'm looking at a system to install to produce our own. Anyone have any recommendations of a good, cost effective and efficient system? Thanks! Curt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC CRO
Hello, Does anyone has a list of category can share that is used for IHC CRO comparison? I want to make a table to do comparison after talking to multiple CROs and I don't want to miss something. Just wondering if anyone did this before and can share your table or list? Thanks, Wendy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ISH decal specimens
Hi Kendra- Decal Stat from Statlab is HCl based, therefore is not appropriate for subsequent IHC, ISH, or other molecular studies. There is lots of peer-reviewed published data, if you need to supply rationale to your pathologists. HCl is known to significantly reduce molecular assay sensitivity and cause false negatives. Switch to either a formic-acid based decal (such as Immunocal) or EDTA-based decal and re-optimize your assay(s). Val Tolley, HTL (ASCP), QIHC > On Jun 23, 2023, at 8:53 AM, Kendra Beechie ND-Bismarck via Histonet > wrote: > > Hello all, > > Recently, the Kappa/Lambda ISH has quit working on our decal specimens. > When we first validated ISH, we tested decal specimens that stained > appropriately. We use the DECAL Stat from StatLab. I did NOT do the initial > ISH validation back in 2019, but as far as I know, we have not changed > which Decal solution we use since then. > > Has anyone else experienced this problem? I realize the other option is to > run K/L IHC on the decal specimens, but we discontinued those after we > brought the ISH on board. My medical director wants me to try to isolate > the problem. My troubleshooting has only revealed that the decal is the > common denominator. > > Thanks for any help! > > Caution: This email is both proprietary and confidential, and not intended > for transmission to (or receipt by) any unauthorized person(s). If you > believe that you have received this email in error, do not read any > attachments. Instead, kindly reply to the sender stating that you have > received the message in error. Then destroy it and any attachments. Thank you. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ISH decal specimens
Hello all, Recently, the Kappa/Lambda ISH has quit working on our decal specimens. When we first validated ISH, we tested decal specimens that stained appropriately. We use the DECAL Stat from StatLab. I did NOT do the initial ISH validation back in 2019, but as far as I know, we have not changed which Decal solution we use since then. Has anyone else experienced this problem? I realize the other option is to run K/L IHC on the decal specimens, but we discontinued those after we brought the ISH on board. My medical director wants me to try to isolate the problem. My troubleshooting has only revealed that the decal is the common denominator. Thanks for any help! Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
