[Histonet] Happy Monday! What Can I Do to HELP YOU?

2024-05-20 Thread relia1--- via Histonet 
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Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block

2024-05-20 Thread Bernice Frederick via Histonet 
Exactly, I process the agar,not the tissue in it Cells,organoids, spheroids.
Bernice

-Original Message-
From: Colleen Forster via Histonet  
Sent: Friday, May 17, 2024 3:07 PM
To: Jay Lundgren 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin 
block

Chistopher,

Were these small pieces of tissue? If they are in an agar (such as
histogel) they would need to have been processed overnight to ensure the agar 
is completely dehydrated during processing. The sample, no matter how small, is 
protected and processes perfectly. IF you ran a short run with the agar it will 
not have [processed properly. I was never able to salvage those samples. It 
took me a couple times to figure out the solution.

Just a thought.

Colleen Forster HT(ASCP)QIHC

On Fri, May 17, 2024 at 2:32 PM Jay Lundgren via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Where did the agarose come from?
>
> On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet < 
> histonet@lists.utsouthwestern.edu> wrote:
>
> >
> >
> > Hello everyone!
> >
> >  I'm having trouble sectioning tissue array blocks where the array 
> > is in agarose embedded into a paraffin block.  I've chilled the 
> > blocks and I'm sectioning on a rotary microtome, at 5 microns, with 
> > a high profile Accuedge blade. The paraffin surrounding the agarose 
> > sections normally,
> but
> > the agarose portion of the block causes the blade to "skip" across 
> > it slightly and even chip out as if my blade isn't snug in the blade 
> > holder (it is).  If I do get a tiny portion of agarose on my section 
> > to float
> out
> > on the waterbath it flies away (like adding ETOH to a waterbath with
> > sections already on it.)   Everyone I have asked about this says the
> > agarose should section normally with the paraffin like any other 
> > FFPE blocks. Any ideas on why this agarose is behaving this way for 
> > me?  Thank you in advance!
> >
> >
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/
> > listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SEM0o5vbbKuzHCJUAy39eR
> > ALW6YwlWuJkF7rTMW9nJ_DRMWtgXqBRmD4yuNChwCHd4H_uCmrPk4dybLFdX60UH_YOK
> > Z8ESaaEB3125Zq$
> >
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> EB3125Zq$
>


--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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Re: [Histonet] [External] Re: Agarose embedded tissue arrays embedded in paraffin block

2024-05-20 Thread Otto, Christopher M via Histonet 
Hi Colleen,

they are blocks of spheroids so they are pretty small.  I don't know how they 
were processed or how the agarose was prepared.

The samples you are talking about, that you ran a short run on, how did that 
behave when you tried to section on the microtome?

Thanks
-Chris

From: Colleen Forster 
Sent: Friday, May 17, 2024 3:06 PM
To: Jay Lundgren 
Cc: Otto, Christopher M ; 
histonet@lists.utsouthwestern.edu 
Subject: [External] Re: [Histonet] Agarose embedded tissue arrays embedded in 
paraffin block

You don't often get email from cfors...@umn.edu. Learn why this is 
important
Chistopher,

Were these small pieces of tissue? If they are in an agar (such as histogel) 
they would need to have been processed overnight to ensure the agar is 
completely dehydrated during processing. The sample, no matter how small, is 
protected and processes perfectly. IF you ran a short run with the agar it will 
not have [processed properly. I was never able to salvage those samples. It 
took me a couple times to figure out the solution.

Just a thought.

Colleen Forster HT(ASCP)QIHC

On Fri, May 17, 2024 at 2:32 PM Jay Lundgren via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:
Where did the agarose come from?

On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet <
histonet@lists.utsouthwestern.edu> 
wrote:

>
>
> Hello everyone!
>
>  I'm having trouble sectioning tissue array blocks where the array is in
> agarose embedded into a paraffin block.  I've chilled the blocks and I'm
> sectioning on a rotary microtome, at 5 microns, with a high profile
> Accuedge blade. The paraffin surrounding the agarose sections normally, but
> the agarose portion of the block causes the blade to "skip" across it
> slightly and even chip out as if my blade isn't snug in the blade holder
> (it is).  If I do get a tiny portion of agarose on my section to float out
> on the waterbath it flies away (like adding ETOH to a waterbath with
> sections already on it.)   Everyone I have asked about this says the
> agarose should section normally with the paraffin like any other FFPE
> blocks. Any ideas on why this agarose is behaving this way for me?  Thank
> you in advance!
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930






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